Investigation of antimicrobial activity of silver and copper citrate for the development of pharmaceuticals preparations


  • Z. N. Polova Bogomolets National Medical University,



Anti Bacterial Agents, Silver, Copper, Citrates


The fast formation of microbial resistance to antibiotics creates necessity of search for new alternative antimicrobial drugs.

Aim. To study the specific antimicrobial activity of silver citrate and copper citrate for the purpose of pharmaceutical drugs developing.

Methods and results. The antimicrobial activity of silver and copper citrate was studied using the standard method of serial dilutions in culture medium at different test strains of microorganisms.

Results. The most sensitive to the silver citrate solution were the strain of Escherichia coli ATCC 25922 (F-50) and Pseudomonas aeruginosae ATCC 9027 at concentration 10 mcg/ml of silver in solution. Sensitivity of Staphylococcus aureus was detected at a concentration of 25 mcg/ml. The results obtained in the experiment indicated that the minimum inhibitory concentration of silver citrate (silver content of 500 mcg/ml) to the test strain of Staphylococcus aureus is 20 mcg/ml, relative to Escherichia coli ATCC 25922 - 6.7 mcg/ml, and a Pseudomonas aeruginosae ATCC 9027 -10 mcg/ml respectively. Staphylococcus aureus is sensitive to solution of silver and copper citrate at 1/20 dilution with content of metal 12.5 mcg/ml. The strain of Escherichia coli ATCC 25922 was sensitive to the test composition at 1/50dilution, which corresponds to the content of metals (silver and copper) - 5 mcg/ml.

Conclusions. Researched solutions demonstrated antimicrobial activity against gram-positive and gram-negative microorganisms. The dependence of the antimicrobial action of the exposure time has been found. These researches give the opportunity to choose the dose of the active pharmaceutical ingredient in the composition of antimicrobial liquid and semi-solid dosage forms.


Babushkina, I. V., Borodulin, V. В., Korschunov, G. V., & Puchinyan, D. M. (2010) Izuchenie antibakterial′nogo dejstviya nanochastic medi i zheleza na klinicheskie shtammy Staphylococcus aureus [Comparative study of antibacterial action of iron and copper nanoparticles on clinical Staphylococcus aureus strains]. Saratovskij nauchno-medicinskij zhurnal, 1(6), 11–14. [in Russian].

Volianskyi, Yu. L., Hrytsenko, I. S., Shyrobokov, V. P., et al. (2004) Vyvchennia spetsyfichnoi aktyvnosti protymikrobnykh likarskykh zasobiv: metodychni rekomendatsii MOZ Ukrainy [Studying of the specific activity of antimicrobial drugs]. Kyiv. [in Ukrainian].

Luna, V., Hall, T., King, D., & Cannons, A. (2010). Susceptibility of 169 USA300 methicillin-resistant Staphylococcus aureus isolates to two copper-based biocides, CuAL42 and CuWB50. Journal Of Antimicrobial Chemotherapy, 65(5), 939–941. doi: 10.1093/jac/dkq092.

Paladini, F., Pollini, M., Sannino, A., & Ambrosio, L. (2015). Metal-Based Antibacterial Substrates for Biomedical Applications. Biomacromolecules, 16(7), 1873–1885. doi: 10.1021/acs.biomac.5b00773.

Koburger, T., Hubner, N., Braun, M., Siebert, J., & Kramer, A. (2010). Standardized comparison of antiseptic efficacy of triclosan, PVP-iodine, octenidine dihydrochloride, polyhexanide and chlorhexidine digluconate. Journal Of Antimicrobial Chemotherapy, 65(8), 1712–1719. doi: 10.1093/jac/dkq212.

How to Cite

Polova ZN. Investigation of antimicrobial activity of silver and copper citrate for the development of pharmaceuticals preparations. Current issues in pharmacy and medicine: science and practice [Internet]. 2016Mar.9 [cited 2024Jun.16];(1). Available from:



Experimental and clinical pharmacology