Chromato-mass-spectometric determination of components of thick extracts from immature walnut fruit


  • Ye. V. Zalygina "Dnepropetrovsk Medical Academy of the Ministry of Health of Ukraine, Dnipro,
  • O. A. Podpletnya "Dnepropetrovsk Medical Academy of the Ministry of Health of Ukraine, Dnipro,
  • K. V. Sokolova "Dnepropetrovsk Medical Academy of the Ministry of Health of Ukraine, Dnipro,



plant extracts, walnut, mass-spectrometry


Topicality. To date, one third of medicines are derived from medicinal plant raw materials and every year the medicinal plants continue to cause increasing interest among doctors. Phytopreparations are attracted by the fact that their use is accompanied by a minimum of side effects, they do not interact with other medicines, they help restore and normalize vital processes, provide the body with the necessary minerals and vitamins, maintain a certain level of metabolism and are successfully used in many chronic diseases.

Aim. The purpose of the study was to study the composition of BAS in dense extracts from immature walnut fruit and clarify their role in the restoration and normalization of vital processes in the human body.

Materials and methods. Dense aqueous extract and thick water-alcohol extracts with an extractant concentration of 30 % ethyl alcohol, 70 % and 96 % were obtained on the basis of the NPAU under the guidance of Professor V.A. Georgiyants, by means of complex processing of immature fruits of milk-wax ripeness of walnut. Determination of the content of BAS in the thick extracts studied was carried out by chromatography-mass spectrometry.

Results. In the study of extracts by chromatography-mass spectrometry, 23 substances were found in the aqueous extract; in water-alcoholic with concentration of extractant - ethyl alcohol 30%, 70% and 96% - 16 34 and 39 compounds, respectively.

Conclusions. By the method of chromatography-mass spectrometry in an aqueous extract of immature walnut fruit 23 substances were detected, in water-alcoholic (30, 70 and 96%) – 16 34 and 39, respectively. Among these compounds we paid attention to 1,4-naphthoquinone, 1-hydroxy-anthraquinone, lilac and lauric acids, diethyl oxalate, eugenol, squalene, juglone - substances with various pharmacological properties.





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How to Cite

Zalygina YV, Podpletnya OA, Sokolova KV. Chromato-mass-spectometric determination of components of thick extracts from immature walnut fruit. Current issues in pharmacy and medicine: science and practice [Internet]. 2018Jun.20 [cited 2024Jun.16];(2). Available from:



Original research