Chromato-mass-spectometric determination of components of thick extracts from immature walnut fruit


  • Ye. V. Zalygina "Dnepropetrovsk Medical Academy of the Ministry of Health of Ukraine, Dnipro,
  • O. A. Podpletnya "Dnepropetrovsk Medical Academy of the Ministry of Health of Ukraine, Dnipro,
  • K. V. Sokolova "Dnepropetrovsk Medical Academy of the Ministry of Health of Ukraine, Dnipro,



plant extracts, walnut, mass-spectrometry


Topicality. To date, one third of medicines are derived from medicinal plant raw materials and every year the medicinal plants continue to cause increasing interest among doctors. Phytopreparations are attracted by the fact that their use is accompanied by a minimum of side effects, they do not interact with other medicines, they help restore and normalize vital processes, provide the body with the necessary minerals and vitamins, maintain a certain level of metabolism and are successfully used in many chronic diseases.

Aim. The purpose of the study was to study the composition of BAS in dense extracts from immature walnut fruit and clarify their role in the restoration and normalization of vital processes in the human body.

Materials and methods. Dense aqueous extract and thick water-alcohol extracts with an extractant concentration of 30 % ethyl alcohol, 70 % and 96 % were obtained on the basis of the NPAU under the guidance of Professor V.A. Georgiyants, by means of complex processing of immature fruits of milk-wax ripeness of walnut. Determination of the content of BAS in the thick extracts studied was carried out by chromatography-mass spectrometry.

Results. In the study of extracts by chromatography-mass spectrometry, 23 substances were found in the aqueous extract; in water-alcoholic with concentration of extractant - ethyl alcohol 30%, 70% and 96% - 16 34 and 39 compounds, respectively.

Conclusions. By the method of chromatography-mass spectrometry in an aqueous extract of immature walnut fruit 23 substances were detected, in water-alcoholic (30, 70 and 96%) – 16 34 and 39, respectively. Among these compounds we paid attention to 1,4-naphthoquinone, 1-hydroxy-anthraquinone, lilac and lauric acids, diethyl oxalate, eugenol, squalene, juglone - substances with various pharmacological properties.





Kovalov, V. M., Pavlii, O. I., & Isakova, T. I. (2000) Farmakohnoziia z osnovamy biokhimii roslyn [Pharmacognosy with the basics of biochemistry of plants]. Kharkiv: Prapor. [in Ukrainian].

Solodovnychenko, N. M., Zhuravlov, M. S., & Kovalov, V. M. (2001) Likarska roslynna syrovyna ta fitopreparaty [Medicinal herbs and herbal]. Kharkiv: Zoloti storinky. [in Ukrainian].

Minarechenko, V. M., & Sereda, P. I. (2004) Resursoznavstvo. Likarski roslyny [Resource-learning. Medicinal plants. educational and methodical manual]. Kyiv : Fitosoitsiotsentr. [in Ukrainian].

Semak, B. B., Barna, M. Yu., & Demkevych, L. I. (2011) Vitchyzniannyi rynok likarskoi roslynnoi syrovyny:problemy i rishennia [The domestic market of herbal resourses: problems and solutions]. Naukovyi visnyk NLTU Ukrainy, 21.1, 264–268. [in Ukrainian].

Murav'eva, D. A., Samylina, I. S., & Yakovlev, G. P. (2002) Farmakognoziya [Pharmacognosy]. Moscow: Medicina. [in Russian].

Safonov, M. M. (2010) Povnyi atlas likarskykh roslyn [Full atlas of medicinal plants]. Ternopil: Navchalna knyha – Bohdan. [in Ukrainian].

Zalygina, E. V., & Podleletnyaya, E. A. (2016) Eksperymentalne doslidzhennia vplyvu hustoho ekstraktu z nezrilykh plodiv horikha voloskoho na funktsionalni pokaznyky roboty shlunkovo-kyshkovoho traktu [Experimental study of the effect of dense extract from immature walnut fruit on functional parameters of the gastrointestinal tract]. Proceedings of the National Congress of Pharmacists of Ukraine, (P. 45). [in Ukrainian].

Zalygina, E. V., Koshova, I. P., & Podpletnyaya, E. A. (2017) Antimicrobial activity of thick aqueous-alcoholic extract of unripe walnut fruits. East European Scientific, 1.

Zalygina, E. V., Podpletnyaya, E. A., & Slesarchuk, V. U. (2017) Doslidzhennia hostroi toksychnosti ta protyzapalnoi dii ekstraktu hustoho vodno-spyrtovoho z nezrilykh plodiv horikha voloskoho [Acute toxicity and anti-inflammatory effect study of unripe walnut fruit thick water-alcohol]. Ukrainskyi biofarmatsevtychnyi zhurnal, 2(49), 44–48. [in Ukrainian]. doi: 10.24959/ubphj.17.108.

Zalygina, E. V., & Podpletnyaya, E. A. (2016) Skryninhove doslidzhennia protyvyrazkovoi aktyvnosti hustykh ekstraktiv nezrilykh plodiv horikha voloskoho [Screening study of antiulcer activity of thick extracts from unripe fruits of walnut]. Farmakolohiia ta likarska toksykolohiia, 6, 47–52. [in Ukrainian].

Zalygina, E. V. (2017) Porivnialne vyvchennia toksychnosti hustoho vodno-spyrtovoho ekstraktu z nezrilykh plodiv horikha voloskoho ta preparatu Altan za povtornoho vedennia shuram [A comparative study of the toxicity of a thick water-alcohol extract from immature fruits of walnut and Altan for repeated nursing]. Farmakolohiia ta likarska toksykolohiia, 6(56), 72–82. [in Ukrainian].

Zalygina, E. V., Slesarchuk, V. U., Podpletnyaya, E. A., & Babanina, N. U. (2018) Perspektyvy stvorennia novykh protyvyrazkovykh likarskykh zasobiv na osnovi biolohichno aktyvnykh rechovyn horikha voloskoho [Prospects for the creation of new anti-ulcer drugs based on biologically active substances of walnut nuts]. Liky – liudyni. Suchasni problemy farmakoterapii i pryznachennia likarskykh zasobiv Proceedings of the 2nd International Scientific and Practical Conference [in Ukrainian].

How to Cite

Zalygina YV, Podpletnya OA, Sokolova KV. Chromato-mass-spectometric determination of components of thick extracts from immature walnut fruit. CIPM [Internet]. 2018Jun.20 [cited 2023Dec.1];(2). Available from:



Original research