Chromatography-mass spectroscopy of larvae of large wax moths


  • Yu. I. Kornievsky Zaporizhzhia State Medical University, Ukraine,
  • О. I. Panasenko ZaporizhzhiaState Medical University, Ukraine,
  • V. G. Kornievskaya Zaporizhzhia State Medical University, Ukraine,
  • N. Yu. Boguslavskaya Zaporizhzhia Regional Perinatal Center, Ukraine,



chromatography-mass spectroscopy, tincture of wax moth larvae, peak area


Tincture of larvae of large wax moth, as a folk remedy, is known since the middle of the 17th century and was used to treat tuberculosis, chronic bronchopulmonary diseases (including pediatrics), when conventional treatment (antibiotics or other chemotherapeutic methods) does not give a positive result, in obstetrics and gynecology in the treatment of climacteric disorders, infertility, miscarriage and many other diseases, as well as means of delaying aging.

The purpose of the work is to determine the component composition of the tincture made of larvae of large wax moth harvested raw materials using gas chromatography (Tomakivka, Dnipro region).

Materials and methods. Raw materials (wax moth larvae) were harvested in September 2017 (Tomakivka, Dnipro region). The tincture was prepared from wax moth larvae according to the method of making tinctures. The tincture was tested on an Agilent 7890B gas chromatograph with a mass spectrometer detector 5977B. To identify the components, the NIST14 mass spectrometer library was used.

Results. Using chromatography-mass-spectrometric identificationin the tincture of moth wax larvae identified 48 components of the main components are: glycerin – 100 %; 1-butanol, 3-methyl – 77.79 %; ethyl oleate – 49.01 %.; еthyl ester of hexadecanoic acid –47.07 %; 1,3-вenzothiazole-6-amino, 2-methyl-7-nitro-18.69 %; feniletyl alcohol – 11.41 %;N, N-dimethylaminoethanol 10.54 %; silane, trimethyl (1-phenyliletil) – 10.50 %; ethyl ester of linolenic acid – 9.26 %; benzenecetaldehyde – 8.99 %.

Conclusions. Using GLC (gas-liquid chromatography), 48 components were established in the wax moth tincture, of which the main 10 components, further in-depth preclinical research is needed to use wax moth tincture in medical practice.



Kondrashova, M. N., et al. (2001) Vozrozhdenie protivotuberkuleznogo, serdechnogo i obshheukreplyayushhego lechebnogo sredstva iz lichinok voskovoj moli [Revival of anti-tuberculosis, cardiac and general restorative remedies from wax moth larvae]. Ot sovremennoj fundamental'noj biologii k novym naukoemkim tehnologiyam. Proceedings of the Conference, (P. 65–66). Puschino. [in Russian].

Sirota, T. V., et al. (2002) Issledovanie antioksidantnyh svojstv preparata narodnoj mediciny iz lichinok voskovoj moli Galleria Mellonella [Study of the antioxidant properties of the folk medicine from wax moth larvae Galleria Mellonella]. Bioantioksidant. Abstracts of Papers of the 6rd International Conference, (P. 528–530). Moscow. [in Russian].

Purygin, P. P., Sribnaja, О. S., Кlenova, N. А., Khudyakova, D. N., Litvinova, Е. G., Коndrashova, M. N., & Оvsepjan, А. А. (2007) Vydelenie antibakterial'nykh komponentov iz gemolimfy lichinok Galleria Mellonella [Excreting antibacterial components fromhemolymph of larvae ofgalleria mellonella]. Vestnik Samarskogo gosudarstvennogo universiteta. Estestvennonauchnaya seriya 9/1(59), 270. [in Russian].

Karneev, F. D. (1999) Dary voskovoj moli [Gifts of wax moth]. Pchelovodstvo, 4, 55–56. [in Russian].

Kondrashova, M. N. (1991) Issledovanie mekhanizma dejstviya preparatov, razrabotannyh S.A. Mukhinym [Investigation of the mechanism of action of drugs developed by S.A. Mukhin]. Proceedings of the 1st Scientific and Practical Conference on the problems of homeopathy, (P. 123–125). Rostov-na-Donu. [in Russian].

Malinovskij, A. A., & Ugreckij, G. E. (1991) S.A. Mukhin i ego delo [S.A. Mukhin and his case]. Proceedings of the 1st Scientific and Practical Conference on the problems of homeopathy, (P. 119–122). Rostov-na-Donu. [in Russian].

How to Cite

Kornievsky YI, Panasenko ОI, Kornievskaya VG, Boguslavskaya NY. Chromatography-mass spectroscopy of larvae of large wax moths. CIPM [Internet]. 2018Jun.20 [cited 2023Dec.1];(2). Available from:



Original research