Chromatography-mass spectroscopy of larvae of large wax moths
Keywords:chromatography-mass spectroscopy, tincture of wax moth larvae, peak area
Tincture of larvae of large wax moth, as a folk remedy, is known since the middle of the 17th century and was used to treat tuberculosis, chronic bronchopulmonary diseases (including pediatrics), when conventional treatment (antibiotics or other chemotherapeutic methods) does not give a positive result, in obstetrics and gynecology in the treatment of climacteric disorders, infertility, miscarriage and many other diseases, as well as means of delaying aging.
The purpose of the work is to determine the component composition of the tincture made of larvae of large wax moth harvested raw materials using gas chromatography (Tomakivka, Dnipro region).
Materials and methods. Raw materials (wax moth larvae) were harvested in September 2017 (Tomakivka, Dnipro region). The tincture was prepared from wax moth larvae according to the method of making tinctures. The tincture was tested on an Agilent 7890B gas chromatograph with a mass spectrometer detector 5977B. To identify the components, the NIST14 mass spectrometer library was used.
Results. Using chromatography-mass-spectrometric identificationin the tincture of moth wax larvae identified 48 components of the main components are: glycerin – 100 %; 1-butanol, 3-methyl – 77.79 %; ethyl oleate – 49.01 %.; еthyl ester of hexadecanoic acid –47.07 %; 1,3-вenzothiazole-6-amino, 2-methyl-7-nitro-18.69 %; feniletyl alcohol – 11.41 %;N, N-dimethylaminoethanol 10.54 %; silane, trimethyl (1-phenyliletil) – 10.50 %; ethyl ester of linolenic acid – 9.26 %; benzenecetaldehyde – 8.99 %.
Conclusions. Using GLC (gas-liquid chromatography), 48 components were established in the wax moth tincture, of which the main 10 components, further in-depth preclinical research is needed to use wax moth tincture in medical practice.
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