Determination of riboflavin in dragee “Revit” by HPLC

Authors

DOI:

https://doi.org/10.14739/2409-2932.2022.2.258888

Keywords:

riboflavin, dragee, vitamins, analysis, liquid chromatography, HPLC

Abstract

The aim of the work is development of express, selective, reproducible methods for the determination of riboflavin by HPLC in a multivitamin preparation, dragee “Revit”.

Materials and methods. Degasser, binary pump, autosampler, thermostated column compartment, diode array detector. Chromatographic column Zorbax SB-C18, 30 mm × 4.6 mm, 1.8 µm. Samples were prepared using analytical electronic scales Kern ABT 100-5M, ultrasonic bath Ultrasonic XUBA 3, nylon ultrafilters 0.12 µm.

Results. 0.1 % formic acid was selected as a buffer solution in the eluent. The maximum values of logD were observed at intervals for riboflavin at pH from 2 to 4. Therefore, the maximum retention of the riboflavin was observed when using the eluent, рН which corresponds to this interval. The pH value for 0.1 % formic acid solution was 2.7. Therefore, such a buffer was chosen by us as part of the eluent. Acetonitrile was selected as the organic modifier. The optimal content of the compound was observed at 10 % acetonitrile.

Conclusions. The chromatographic behavior of riboflavin on the basis of chemical-analytical properties was studied. The optimal conditions for chromatographic determination were determined and a specific, express method for the determination of riboflavin in “Revit” dragee was developed. The technique on a real sample of “Revit” dragee was elaborated. The method was recommended for use in control and analytical and research laboratories.

 

Author Biographies

B. О. Varynskyi, Zaporizhzhia State Medical University, Ukraine

PhD, DSc, Associate Professor of the Department of Physical and Colloidal Chemistry

M. Yu. Zeleniuk, Zaporizhzhia State Medical University, Ukraine

Master student of the 5th course of Pharmaceutical Faculty

A. H. Kaplaushenko, Zaporizhzhia State Medical University, Ukraine

PhD, DSc, Professor, Head of the Department of Physical and Colloidal Chemistry

References

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Published

2022-08-01

How to Cite

1.
Varynskyi BО, Zeleniuk MY, Kaplaushenko AH. Determination of riboflavin in dragee “Revit” by HPLC. Current issues in pharmacy and medicine: science and practice [Internet]. 2022Aug.1 [cited 2024Nov.26];15(2):140-4. Available from: http://pharmed.zsmu.edu.ua/article/view/258888

Issue

Vol. 15 No. 2 (2022)

Section

Original research

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