The study of chemical composition of Centaurea diffusa Lam.


  • V. I. Mozul Zaporizhzhia State Medical University, Ukraine,
  • I. I. Aksonova Zaporizhzhia State Medical University, Ukraine,
  • O. I. Panasenko Zaporizhzhia State Medical University, Ukraine,



Centaurea, gas chromatography, plant bioactive compounds



The aim of the work is to study the chemical composition of biologically active compounds of Centaurea diffusa Lam. and the future prospects of its application in the medical and pharmaceutical practice.

Materials and methods. The object of the study is the grass of Centaurea diffusa Lam., which was collected in Zaporizhia in July 2018. The method of high-performance gas chromatography on the device Agilent 7890B GC System (Agilent, Santa Clara, CA, USA) with mass spectrometry detector Agilent 5977 BGC / MSD (Agilent, Santa Clara, CA, USA) was used to determine the qualitative and quantitative composition of Centaurea diffusa Lam.

Results. It was discovered that raw material of Centaurea diffusa Lam. contains 55 components, which are representatives of different classes of biologically active substances. There are phenols – 9, alcohols – 9, carboxylic acids (saturated and unsaturated) – 8, esters – 7, alkanes – 5, heterocyclic compounds – 4, ketones – 4, terpenes – 3, aldehydes – 2, alkenes – 1, monosaccharides – 2, hydrazides – 1. However, these data differ from the total content of compounds by percentage. Phenols make up 21.45 % and this is the highest indicator, carboxylic acids (saturated and unsaturated) are 19.71 %, and alcohols and esters are 17.63 % and 12.43 % respectively. Retusin constitutes the main part among all found compounds – 15.53 %, 4-hexadecanoic acid (12.25 %) and 2-ethyl-2-(hydroxymethyl)-1,3-propanediol (8.17 %) are on the second and third place. It’s useful to note that hexadecanoic acid 2-hydroxy-1-(hydroxymethyl)ethyl ester is present in quantity 4.3 %.

Based on the results obtained, it is possible to consider the further prospects of using Centaurea diffusa Lam. in medical and pharmaceutical practice.

Conclusions. The chemical composition of grass of Centaurea diffusa Lam. was first set by the method of gas chromatography with mass spectrometry detector. The presence of 55 biologically active components was determined. The experimental results prove the prospects and relevance of studies the chemical composition of Centaurea diffusa Lam. in order to create new herbal remedies based on it.



Krechun, A. V., Mykhailenko, O. O., Kovalov, S. V.,  Orlova, T. G. (2019). Skryninh nakopychennia biolohichno aktyvnykh rechovyn v irysi uhorskomu vprodovzh vehetatsiinoho periodu [Screening of accumulation of biologically active substances of Iris hungarica during vegetation period]. Current issues in pharmacy and medicine: science and practice, 12, 2(30). 135-140. doi: 10.14739/2409-2932.2019.2.170975 [in Ukrainian].

Luchkiv, N. Yu. (2017). Vpliv ekologo- tsenotychnykh umov na khimichnyi sklad roslynnoi syrovyny Centaurea sarpatica Porc [Influence of ecological-coenotic conditions on the chemical composition of herbal seria Centaurea carpatica Porc]. Bukovyna Medical Bulletin, 21(1), 71-75 [in Ukrainian]/

Marchyshyn, S. M., Stoiko, L. І., Skrynchuk, O. Ya.,  Rakhmetov, D. B. (2019). Doslіdzhennja yakisnogo skladu ta kіlkіsnogo vmіstu kislot zhyrnykh katranu sercelystogo ta katranu koktebelskogo lystkіv [Research of qualitative composition and quantitative content of fatty acids of Crambe cordifolia Steven and Crambe koktebelica (Junge) N. Busch leaves]. Current issues in pharmacy and medicine: science and practice, 12(1), 15-20. doi: 10.14739/2409-2932.2019.1.158939 [in Ukrainian].

Ertas, A., Gören, A. C., Boga, M., Demirci, S., & Kolak, U. (2014). Chemical composition of the essential oils of three Centaurea species growing wild in Anatolia and their anticholinesterase activities. Journal of Essential Oil Bearing Plants, 17 (5), 922-926. doi: 10.1080/0972060X.2014.886164

Chemsa, A. E., Zellagui, A., Öztürk, M., Erol, E., Ceylan, O., & Duru, M. E. (2018). The chemical composition of Centaurea Furfuracea Coss. & dur. essential oil with antioxidant, anticholinesterase and antibiofilm activities. Journal of ongoing chemical research, 3(2), 54-62. id: 2018JOCR25.

Beltagy, A. M. (2015). Chemical composition and cytotoxic activity of Centaurea Scoparea Sieb against four human cell lines. Journal of Pharmaceutical Sciences and Research., 7(3), 103-107.

Belbache, H., Mechehoud, Y., Chalchat, J. C., Figueredo, G., Chalard, P., Benayache, S. et al. (2017). Essential oil composition of Centaurea sempervirens L. (Asteraceae). International Journal of Pharmacognosy and Phytochemical Research., 9(1), 79-82. doi: 10.25258/ijpapr.v9i1.8045

Nicholas, D. A., Zhang, K., Hung, C., Glasgow, S., Aruni, A. W., Unternaehrer J. K. et al. (2017). Palmitic acid is a toll-like receptor 4 ligand that induces human dendritic cell secretion of IL-1β. PLoS One, 12(5). doi: 10.1371/journal.pone.0176793.

Thirunavukkarasu, K., Rajkumar, P., Selvaraj, S., Kumaresan, S. (2016). GC-MS analysis of Gymnema sylvestre leaves methanolic extract for antidiabetic and anticancer drug identification. Journal of Chemical and Pharmaceutical Sciences, 9(2), 1011-1013.

Lockowandt, L., Pinela, J., Roriz, C., Pereira, C., Abreu, R., & Calhelha, R. et al. (2019). Chemical features and bioactivities of cornflower (Centaurea cyanus L.) capitula: The blue flowers and the unexplored non-edible part. Industrial Crops And Products, 128, 496-503. doi: 10.1016/j.indcrop.2018.11.059

Pshenichnyuk, S., Elkin, Y., Kulesh, N.,  Lazneva, E. (2015). Low-energy electron interaction with retusin extracted from Maackia amurensis: towards a molecular mechanism of the biological activity of flavonoids. Physical Chemistry Chemical Physics, 17(26), 16805-16812. doi: 10.1039/C5CP02890F

Sudha, T., Chidambarampillai, S.,  Mohan, V. R. (2013). GC-MS analysis of bioactive components of aerial parts of Fluggea leucopyrus Willd. (Euphorbiaceae). Journal of Applied Pharmaceutical Science, 3(05), 126-130. doi: 10.7324/JAPS.2013.3524

How to Cite

Mozul VI, Aksonova II, Panasenko OI. The study of chemical composition of Centaurea diffusa Lam. CIPM [Internet]. 2019Nov.21 [cited 2023Dec.8];(3). Available from:



Original research