Microscopic research of shoots of the Salix cinerea L. of Ukrainian flora
Keywords:Salix cinerea L., shoots, microscopic features, SEM
Representatives of the Salicaceae family are valuable sources of medicinal plant material that has been used in traditional medicine in many countries around the world.. In European countries, some willow species are official plants. It is the bark of willow in many countries called natural aspirin. The decoction of the willow bark is used as an antimalarial, antipyretic, wound healing, antiseptic, hemostatic, astringent and anti-inflammatory agent. Plants of the genus Salix L. have a variety of component composition and contain a complex of biologically active compounds: primarily polyphenolic compounds, phenol glycosides, salicylates, flavonoids, hydroxycoric acids, essential oils, vitamins, carotenoids, polysaccharides. The bark of young branches of different willow species, including Salix purpurea L., Salix fragilis L., Salix daphnoides Vill., have been included in the edition of the European Pharmacopoeia and since 2014 to the State Pharmacopoeia of Ukraine. Expanding the raw material base of medicinal plant raw materials is an urgent problem that can be solved through the introduction into the pharmaceutical practice of systematically close autochthonous and introduced willow species widespread inUkraine.
The purpose of this work is the determination of microscopic diagnostic features of the willow goat (Salix cinerea L.) shoots of Ukrainian flora for the plant material identification.
Materials and methods. The object of the study was dry shoots of Salix cinerea L. Vegetable raw materials were collected in May–August 2016–2018 in Kharkiv, Zakarpattia and Kyiv region of Ukraine.
The study of the anatomical structure of goat willow shoots was performed using the methods of light and electron microscopy. The ultrastructure of the epidermal leaf tissue surface was further studied using scanning microscopy techniques.
Results. An in-depth study of the anatomical structure of the shoots of Salix cinerea L. has been carried out and the main diagnostic macro- and microscopic features of perspective medicinal raw materials have been established.
Conclusions. The obtained results significantly expand information on the anatomical structure of shoots of Salix cinerea L. flora of Ukraine and could be used in the standardization of raw materials of species of the family Willow.
Borodina, N. V., & Kovalev, V. N. (2015). Sravnitelnyj analiz fenolnyh soedinenij pobegov Salix caprea L., Salix purpurea L., Salix viminalis L. flory Ukrainy [Comparative analysis of phenolic compounds of Salix caprea L., Salix purpurea L., Salix viminalis L. shoots in Ukrainian flora]. Phenolic compounds: fundamental and applied aspects: Proceedings of the IX International symposium. (pp. 27-33). Moscow. [in Russian].
Borodina, N. V. (2017). Fenolni spoluky Salix cinerea L. [Phenolic compounds of Salix cinerea L.]. The development of science in the 21st century : Proceedings of the International Scientific and Practical Conference. (pp. 66-74). Kharkiv. [in Ukrainian].
Borodina, N. V., Kovalov, V. M., & Koshoviy, O. M. (2019). Doslidzhennia ultrastruktury poverkhni lystkiv 23 vydiv rodyny Verbovi flory Ukrainy [Ultrastructure investigation of the leaf surface of 23 species of the Willow family in Ukrainian flora]. Modern pharmacy: history, realities and prospects of development. Proceedings of the International Scientific and Practical Conference. (Vol. 1 pp. 271-272). Kharkiv. [in Ukrainian].
Hurtovenko, I. O., Konovalova, O. Yu., Scherbakova, O. F., Menshova, V. O., & Hudzenko, O. I. (2018). Doslidzhennia anatomichnykh diahnostychnykh oznak syrovyny deiakykh vydiv rodu Agastache yak pokaznykiv yakosti pry standartyzatsii [The study of anatomical diagnostic signs of raw material of some Agastache species as quality indicators for standardization]. Current issues in pharmacy and medicine: science and practice, 11(2), 230-237. doi: 10.14739/2409-2932.2018.2.133179 [in Ukrainian].
(2014). Derzhavna Farmakopeya Ukrayiny [The State Pharmacopoeia of Ukraine]. Kharkiv. Vol. 3. pp. 263-265. [in Ukrainian].
(2015). Derzhavna Farmakopeya Ukrayiny [The State Pharmacopoeia of Ukraine]. Kharkiv. Vol. 1. P. 1128. [in Ukrainian].
Ishchuk, L. P. (2018). Verba v etnokulturnykh tradytsiyakh i zvychaiah ukraintsiv [Willow in Ukrainian Ethnocultural Traditions and Customs]. Ethnobotanical traditions in agronomy, pharmacy and garden design: Proceedings of the International Scientific Conference. (pp. 100-108). Uman. [in Ukrainian].
Ishchuk, L. P. (2015). Rid Salix L. v Ukraini [Genus Salix L. in Ukraine]. News of the Askania-Nova Biosphere Reserve, 17, 35-43. [in Ukrainian].
Kornilova, O. O., & Makarova, O. Ye. Suchasni problemy standartyzatsii likarskoi roslynnoi syrovyny [Modern standardization problems of medicinal plant raw materials]. Quality Management in Pharmacy: Proceedings of the Scientific and Practical Conference. (pp. 64-65). Kharkiv. [in Ukrainian].
Nedoseko, O. I. (2014). Borealnye vidy iv podrodov Salix i Vetrix: ontomorfogenez i zhiznennye formy [Boreal willow species of the subgenera Salix and Vetrix: ontomorphogenesis and life forms]. Nizhny Novgorod. [in Russian].
Borodina, N V., & Kovalov, V. M. (2018). Mineralnyi sklad deiakykh vydiv rodyny Salicaceae. [Mineral composition of some species of the family Salicaceae]. Collection of scientific works of staff members of NMAPE. (Vol. 29 pp. 180-185). Kyiv. [in Ukrainian].
Kovalov, V. M., & Marchyshyn, S. M. (Eds) (2014). Praktykum z identyfikatsii likarskoi roslynnoi syrovyny [Workshop on the identification of medicinal plant raw materials]. Ternopil. [in Ukrainian].
Smoilovska, H. P., Yerenko, O. K., Khortetska, T. V., Duiun, I. F., & Mazulin O. V. (2019). Mikroskopichni doslidzhennia likarskoi roslynnoi syrovyny dereviiu blido-zhovtoho [Microscopic research of medicinal plant raw material of Achillea ochroleuca Ehrh]. Current issues in pharmacy and medicine: science and practice, 12(1), 25-30. doi: 10.14739/2409-2932.2019.1.158984 [in Ukrainian].
Frolova, O. O., Kompanceva, E. V., & Dementeva, T. M. (2016). Biologicheski aktivnye veshhestva rastenij roda Iva (Salix L.) [Biologically active plant substances of the genus Willow (Salix L.)]. Pharmacy & Pharmacology, 4(2), 41-59. doi: 10.19163/2307-9266-2016-4-2(15)-41-59 [in Russian].
Khortetska, T. V., & Smoilovska H. P. (2017). Mikroskopichni doslidzhennia podorozhnyka velykoho ta podorozhnyka serednoho pry identyfikatsii likarskoi roslynnoi syrovyny [Microscopic research on Plantago major L. and Plantago media L. in identification of medicinal plant-based raw material]. Current issues in pharmacy and medicine: science and practice, 10(1), 54-59. doi: 10.14739/2409-2932.2017.1.93439 [in Ukrainian].
Barthlott, W., Mail, M., Bhushan, B., & Koch, K. (2017). Plant Surfaces: Structures and Functions for Biomimetic Innovations. Nano-Micro Letters, 9(23). doi: 10.1007/s40820-016-0125-1
Council of Europe. (2010). European Pharmacopoeia. Strasbourg.
Kishore, N., Mangilal, T., Anjaneyulu, N., Abhinayani, G., & Sravya, N. (2014) Investigation of anti-inflammatory and invitro antioxidant activities of hydroalcoholic extract of bark of Salix tetrasperma Roxb. Int. J. Pharm. Anal., 2(5), 506-509.
Ramos, P., Moreirinha, C., Santos, S., Almeida, A., Freire, C., Silva, A., & Silvestre, A. (2019). Valorisation of bark lipophilic fractions from three Portuguese Salix species: A systematic study of the chemical composition and inhibitory activity on Escherichia coli. Industrial Crops And Products, 132, 245-252. doi: 10.1016/j.indcrop.2019.02.028
Borodina, N. V., & Golub, V. I. (2015). Phytochemical research of Salix cinerea L. Topical issues of new drugs development: abstracts оf international scientific and practical conference of young scientists and student. (p. 66). Kharkiv.
Borodina, N. (2016). Research of volatile compounds from Salix cinerea L. Oxford Review of Education and Science, 5(1), 468-473.
Borodina, N., Kovalyov, V., Koshovyi, O., & Gamulya, O. (2019). Study of the morphologo-anatomic signs of shoots of the Salix caprea L. of Ukrainian flora. Sciencerise: Pharmaceutical Science, 4(20), 34-44. doi: 10.15587/2519-4852.2019.178319
Sulima, P., Krauze-Baranowska, M., & Przyborowski, J. (2017). Variations in the chemical composition and content of salicylic glycosides in the bark of Salix purpurea from natural locations and their significance for breeding. Fitoterapia, 118, 118-125. doi: 10.1016/j.fitote.2017.03.005
Borodina, N., Koshovyi, O., & Kovalyov, V. (2016). The chromatography-mass spectrometry study of Salix rosmarinifolia L. Azerbaijan Pharmaceutical & Pharmacotherapy Journal, 2, 15-20.
Borodina, N. V., Kovalyov, V. N., Koshovyi, О. N., & Stremoukhov, A. A. (2018). The study of salix myrsinifolia Salisb. Leaves by the gc/ms method / Science and phactice 2018. Proceedings of the 9th International Scientific and Practical Conference. Kaunas.
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