Application of quinone derivatives for spectrophotometric determination of drugs


  • K. P. Miedviedieva Zaporizhzhia State Medical University, Ukraine,
  • А. О. Donchenko Zaporizhzhia State Medical University, Ukraine,
  • S. O. Vasiuk Zaporizhzhia State Medical University, Ukraine,



acetylcysteine, streptomycin sulfate, 2, 3-dichloro-1, 4-napthoquinone, sodium 1, 2-naphthoquinone-4-sulfonate, spectrophotometry, assay, validation studies


The aim of this research is the development and validation of spectrophotometric methods for the determination of acetylcysteine and streptomycin sulfate, suitable for the standardization of test substances and analysis dosage forms.

Materials and methods. The substances of the acetylcysteine and the streptomycin sulfate, the quinone derivatives (the solutions of sodium 1,2-naphthoquinone-4-sulfonate and 2,3-dichloro-1,4-naphthoquinone), as the analytical color reagents, were used for the experiment. The spectrophotometer Specord 200 for measuring the optical density was applied.

Results. According to experimental data, the optimal conditions between the test substances and the added reagents were established. The factors affecting the character of the absorbance spectrum and the value of absorbance: the nature of the solution medium, the amount of added reagents, pH of the reaction mixture, conduction period of the reaction and reaction product resistance in time have been studied for the development of a methods of quantitative determination of acetylcysteine and streptomycin sulfate on based reactions with quinone derivatives.

It was experimentally established that the sodium 1,2-naphthoquinone-4-sulfonate with streptomycin sulfate in the alkaline medium to form a colored composition with maximum light absorbance at 560 nm. The solution of 2,3-dichloro-1,4-naphthoquinone with acetylcysteine in the DMFA medium to form a colored composition with maximum light absorbance at 425 nm. It was necessary to heat it on the water bath for 10 min at 95 °С for more complete conduction period of reaction.

According to SPU, the procedure of validation was determined. It has been proved that the developed techniques of the quantitative determination based on such characteristics as linearity, accuracy, validity, application range and robustness. The obedience to the Beer’s Law is 4.48–8.40 mg/100 ml for acetylcysteine and 2.00–8.00 mg/100 ml for streptomycin sulfate. Stoichiometric ratios of the reaction mixture component have been established by means of the method of isomolar series and continuous change method.

Conclusion. As a result of this work, the optimal reaction conditions for the reaction of acetylcysteine with 2,3-dichloro-1,4-naphthoquinone and streptomycin sulfate with sodium 1,2-naphthoquinone-4-sulfonate have been established. Based on the obtained data, new spectrophotometric methods for the quantitative determination of the investigated drugs were developed and tested on the dosage forms. It is proved that the developed methods are simple, reproducible and expressive.


Miedviedieva, K. P. (2015). Rozrobka spektrofotometrychnykh metodyk kilkisnoho vyznachennia likarskykh rechovyn shcho mistiat pervynnu alifatychnu aminohrupu (Dis… kand. farm. nauk). [Spectrophotometric method development for the quantitative determination of drugs containing the primary aliphatic amino group. Dr. farm. sci. diss.]. Zaporizhzhia. [in Ukrainian].

Donchenko, A. O., Vasyuk, S. O., & Portna, K. P. (2015). Vykorystannia 2,3-dykhlor-1,4-naftokhinonu dlia spektrofotometrychnoho vyznachennia atsetyltsysteinu v likarskykh preparatakh [Spectrophotometric determination of acetylcysteine in pharmaceutical formulations using 2,3-dichloro-1,4-napthoquinone]. Current issues in pharmacy and medicine: science and practice, 1, 36-39. doi:

Rani M. (2014). Spectrophotometric determination of cefadroxil with 2,3 - dichloro-5,6 dicyano-1, 4-benzoquinone. World Journal of Pharmaceutical Research, 3(9), 1196-1200.

Sheikh, R., Amin, A., Gouda, A., & Zahran, D. (2017). Validated spectrophotometric methods for determination of cefdinir in pure and dosage forms through charge transfer complexation using alizarin derivatives. International Journal of Research in Pharmacy and Pharmaceutical Sciences, 2(6), 11-18.

Ali Ahmed, S., Elbashir, A., & Aboul-Enein, H. (2015). New spectrophotometric method for determination of cephalosporins in pharmaceutical formulations. Arabian Journal Of Chemistry, 8(2), 233-239. doi: 10.1016/j.arabjc.2011.08.012

Kumari, N., & Vasundhara, A. (2016). A noval method development for spectrophotometric determination of ertapenem in bulk and injection formulations by NQS. International Journal of Science Technology and Management, 5(1), 1-9.

Rashid, Q. N., Bakir, M. H., & Baban, S. O. (2016). Spectrophotometric determination of Diclofenac Sodium in pure form and in the pharmaceutical preparations. Tikrit Journal of Pure Science, 21(3), 76-80.

Shantier, S. W., Gadkariem, E. A., & Mohamed R. (2015). Development of colorimetric method for the analysis of aminocaproic acid using DCQ. World Journal of Pharmaceutical Research, 4(4), 234-240.

Omar, M., Hammad, M., & Eltoukhi, W. (2017). Spectrophotometric Determination of Certain Antimigraine Drugs in Pharmaceutical Formulations Using p-Chloranil Reagent; Application to Content Uniformity Testing. Analytical Chemistry Letters, 7(5), 611-622. doi: 10.1080/22297928.2017.1372208

Donchenko, A., & Vasyuk, S. (2018). Spectrophotometric determination of metoprolol tartrate in pure and dosage forms. Journal of Faculty of Pharmacy of Ankara University, 42(1), 33-42. doi: 10.1501/eczfak_0000000600

Lima, M., Cassella, R., & Pacheco, W. (2017). Spectrophotometric determination of rosuvastatin in pharmaceutical formulations using quinalizarin. Brazilian Journal Of Pharmaceutical Sciences, 53(3). doi: 10.1590/s2175-97902017000300075

Makka, H. A., Mukhtar, M. E., Choudhary, M. I., Ahmed, S., & Saeed, A. E. M. (2017). Development and validation of spectrophotometric methods for the determination of esomeprazole magnesium in pharmaceutical formulations. World Journal Of Pharmacy And Pharmaceutical Sciences, 6(2), 1344-1359. doi: 10.20959/wjpps20172-8635

Abdulrahman, S., & Basavaiah, K. (2014). Use of alizarin red S as a chromogenic agent for the colorimetric determination of dothiepin hydrochloride in pharmaceutical formulations. Journal Of Saudi Chemical Society, 18(2), 107-114. doi: 10.1016/j.jscs.2011.05.018

Grizodub A. I. (2006). Standartnye procedury validacii metodik kontrolja kachestva lekarstvennyh sredstv [Standard validation procedures for drug quality control]. Farmakom, 1/2, 35-44. [in Russian].

(2015). Derzhavna Farmakopeya Ukrayiny [The State Pharmacopoeia of Ukraine]. Kharkiv. Vol. 1. P. 1128. [in Ukrainian].

Jermer, J., & Miller, D. (2013). Validacija metodik v farmacevticheskom analize. Primery nailuchshej praktiki [Method validation in pharmaceutical analysis. Best practice examples]. Moscow. [in Russian].

How to Cite

Miedviedieva KP, Donchenko АО, Vasiuk SO. Application of quinone derivatives for spectrophotometric determination of drugs. Current issues in pharmacy and medicine: science and practice [Internet]. 2019Nov.21 [cited 2024Jul.21];(3). Available from:



Original research