Application of quinone derivatives for spectrophotometric determination of drugs
Keywords:acetylcysteine, streptomycin sulfate, 2, 3-dichloro-1, 4-napthoquinone, sodium 1, 2-naphthoquinone-4-sulfonate, spectrophotometry, assay, validation studies
The aim of this research is the development and validation of spectrophotometric methods for the determination of acetylcysteine and streptomycin sulfate, suitable for the standardization of test substances and analysis dosage forms.
Materials and methods. The substances of the acetylcysteine and the streptomycin sulfate, the quinone derivatives (the solutions of sodium 1,2-naphthoquinone-4-sulfonate and 2,3-dichloro-1,4-naphthoquinone), as the analytical color reagents, were used for the experiment. The spectrophotometer Specord 200 for measuring the optical density was applied.
Results. According to experimental data, the optimal conditions between the test substances and the added reagents were established. The factors affecting the character of the absorbance spectrum and the value of absorbance: the nature of the solution medium, the amount of added reagents, pH of the reaction mixture, conduction period of the reaction and reaction product resistance in time have been studied for the development of a methods of quantitative determination of acetylcysteine and streptomycin sulfate on based reactions with quinone derivatives.
It was experimentally established that the sodium 1,2-naphthoquinone-4-sulfonate with streptomycin sulfate in the alkaline medium to form a colored composition with maximum light absorbance at 560 nm. The solution of 2,3-dichloro-1,4-naphthoquinone with acetylcysteine in the DMFA medium to form a colored composition with maximum light absorbance at 425 nm. It was necessary to heat it on the water bath for 10 min at 95 °С for more complete conduction period of reaction.
According to SPU, the procedure of validation was determined. It has been proved that the developed techniques of the quantitative determination based on such characteristics as linearity, accuracy, validity, application range and robustness. The obedience to the Beer’s Law is 4.48–8.40 mg/100 ml for acetylcysteine and 2.00–8.00 mg/100 ml for streptomycin sulfate. Stoichiometric ratios of the reaction mixture component have been established by means of the method of isomolar series and continuous change method.
Conclusion. As a result of this work, the optimal reaction conditions for the reaction of acetylcysteine with 2,3-dichloro-1,4-naphthoquinone and streptomycin sulfate with sodium 1,2-naphthoquinone-4-sulfonate have been established. Based on the obtained data, new spectrophotometric methods for the quantitative determination of the investigated drugs were developed and tested on the dosage forms. It is proved that the developed methods are simple, reproducible and expressive.
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