Study of local irritant and sensitizing activity of the amber-based oil complex TM IL SAV AMBER in vivo

Authors

DOI:

https://doi.org/10.14739/2409-2932.2026.2.352352

Keywords:

amber-based oil complex TM IL SAV AMBER, local tolerance, hypersensitivity to amber extracts

Abstract

The aim of the article is to investigate the local irritant effect on the skin and conjunctiva of the eyes of the amber-based oil complex TM IL SAV AMBER and to analyze its sensitizing activity in vivo.

Materials and methods. A series of chronic experiments of different durations (48 hours, 14 days and 4 weeks) were used to study the local irritant effect of the drug in vivo (on white sexually mature mice, 2 groups of animals, one of which was a control, n = 20 – method of skin applications; and on white-colored or white-spotted guinea pigs weighing 300–400 g, 2 groups of animals, one of which was a control, n = 10 – conjunctival test) and to study the sensitizing effect of the drug in vivo (on white-colored guinea pigs weighing 300–400 g, 4 groups of animals, two of which were control, n = 20 – method of repeated skin applications).

Results. Studies of the skin irritation effect of the drug in mice showed that dermal applications of the studied drug for 14 days did not cause signs of inflammation and damage – redness, edema, rashes and ulceration. The general behavior of the experimental animals (mobility, appetite, coat) did not differ from that of the control group. The conjunctival test did not reveal hyperemia and edema of the conjunctiva, sclera and lacrimal duct, and also did not cause immediate-type hypersensitivity reactions. The study of the sensitization properties of the studied drug using dermal applications did not reveal immediate-type and delayed-type hypersensitivity reactions in experimental animals in vivo.

Conclusions. The oil complex based on amber TM IL SAV AMBER did not affect general behavior, did not cause local irritation, and did not exhibit sensitizing activity in the studied animals in vivo.

Author Biographies

Yu. V. Voronina-Tuzovskykh, T. H. Shevchenko National University “Chernihiv Colehium”

PhD, Associate Professor of the Department of Chemistry, Technology and Pharmacy

V. M. Poletai, T. H. Shevchenko National University “Chernihiv Colehium”

PhD, Associate Professor of the Department of Biology and Human Health

O. S. Bondar, T. H. Shevchenko National University “Chernihiv Colehium”

PhD, Associate Professor of the Department of Physics and Astronomy

V. O. Yanchenko, T. H. Shevchenko National University “Chernihiv Colehium”

PhD, Associate Professor of the Department of Chemistry, Technology and Pharmacy

I. O. Savosta, LLC IL SAV AMBER, Kyiv

Director

References

  1. Bielichenko OP., Haievskyi YD. Doslidzhennia burshtynu metodom infrachervonoi spektroskopii [Study of amber by infrared spectroscopy]. Precious and decorative stones. 2012;(4):12-6. Ukrainian. Available from: http://nbuv.gov.ua/UJRN/Ktdk_2012_70_4_5
  2. Palchevska TA, Lysenko YuS, Hula, LD, Razhyk AV. Zastosuvannia burshtynovoi kysloty ta natriiu suktsynatu u farmatsii [Application of succinic acid and sodium succinate in pharmacy]. In: Science, society, education: topical issues and development prospects. 2020 Apr 12-14; Kharkiv, Ukraine: SPC “Sci-conf.com.ua”; 2020. p. 683-7. Ukrainian. Available from: https://er.knutd.edu.ua/handle/123456789/19673?locale=en
  3. Tian Y, Zhou S, Takeda R, Okazaki K, Sekita M, Sakamoto K. Anti-inflammatory activities of amber extract in lipopolysaccharide-induced RAW 264.7 macrophages. Biomed Pharmacother. 2021;141:111854. doi: https://doi.org/10.1016/j.biopha.2021.111854
    | |
  4. Chornyi VM, Mysiura TH, Popova NV, Zavialov VL. [Solvent selection for extraction of target components from amber]. Journal of Chemistry and Technologies. 2021;29(1):92-9. Ukrainian. doi: https://doi.org/10.15421/082106
    |
  5. Chornyi V, Krûma Z, Mysiura T, Popova N, Zavialov V. Comparative extraction of components from Ukrainian and Baltic ambers. Proc Latv Acad Sci Sect B Nat Exact Appl Sci. 2023;77(5-6):244-9. doi: https://doi.org/10.2478/prolas-2023-0035
    |
  6. Mironov OL, Kachalova NM, Dziuba OI, Levchyk NY, Bohza SL. [Research of biological activity of Ukrainian amber]. Fizyko-orhanichna khimiia, farmakolohiia ta farmatsevtychna tekhnolohiia biolohichno aktyvnykh rechovyn. 2018;(1):13-24. Ukrainian. Available from: https://test.knutd.edu.ua/handle/123456789/23539
  7. Levchyk NY, Dziuba OI, Kachalova NM, Mironov OL. Biological acti­vity of aqveous solution of amber. Biotechnol Acta. 2017;10(6):53-60. doi: https://doi.org/10.15407/biotech10.06.053
  8. Kovalevych LA. Teoretychni uzahalnennia oznak i kryteriiv poshuku ta otsinka rodovyshch burshtynu na terytoriii Ukrainy [Theoretical generalization of signs and criteria for the search and evaluation of amber deposits on the territory of Ukraine]. Visnyk ZhDTU, 2007;2(41):1-6. Ukrainian. Available from: https://library.ztu.edu.ua/e-copies/VISNUK/41_II/143.pdf
  9. Voronina-Tuzovskykh YV, Tkachenko SV, Kurmakova IM, Yanchenko VO, Usov AM, Savosta IO, et al. Elementnyi sklad burshtynovoi pudry-abrazyvu ta perspektyvnist yii vykorystannia yak syrovyny farmatsevtychnoi promyslovosti [Elemental composition of amber abrasive powder and perspective its use as a raw material pharmaceutical industry]. Scientific Collection “InterConf+”. 2023;39:461-9. Ukrainian. doi: https://doi.org/10.51582/interconf.19-20.11.2023.049
  10. Voronina-Tuzovskykh Yu, Mikulenko O, Bondar O, Kurmakova І, Pleshakov O. Khimichnyi sklad matseratu na osnovi burshtynu: metody analizu ta rezultaty [Chemical composition of amber-based macerate: methods of analysis and results]. Biota. Human. Technology. 2024;(2):171-79. Ukrainian.
  11. Ministry of Health of Ukraine. Poriadok provedennia doklinichnoho vyvchennia likarskykh zasobiv ta ekspertyzy materialiv doklinichnoho vyvchennia likarskykh zasobiv [Procedure for conducting preclinical studies of medicinal products and examination of materials for preclinical studies of medicinal products. Order dated 2009 Dec 14, No. 944. Available from: https://zakon.rada.gov.ua/laws/show/z0053-10/ed20270101?lang=en#Text
  12. Suckow MA, Stevens KA, Wilson RP. The Laboratory Rabbit, Guinea Pig, Hamster, and Other Rodents. Elsevier; 2012.
  13. Stefanov AV, editor. Doklinichni doslidzhennia likarskykh zasobiv [Preclinical studies of medicinal products]. Kyiv: Avitsena; 2001. Ukrainian.
  14. European Medicines Agency. Guideline on non-clinical local tolerance testing of medicinal products. EMA/CHMP/SWP/2145/2000 Rev. 1, Corr. 1, 22 October 2015.
  15. Kotsyumbas IY, Bisyuk IY, Gorzheev VM, Malik OG, Zhila MI, Kosenko YM, et al. Klinichni doslidzhennia veterynarnykh preparativ ta kormovykh dobavok [Clinical trials of veterinary drugs and feed additives]. Lviv: SAM Publishing House LLC. 2013. Ukrainian.

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Published

2026-06-26

How to Cite

1.
Voronina-Tuzovskykh YV, Poletai VM, Bondar OS, Yanchenko VO, Savosta IO. Study of local irritant and sensitizing activity of the amber-based oil complex TM IL SAV AMBER in vivo. Current issues in pharmacy and medicine: science and practice [Internet]. 2026Jun.26 [cited 2026Jun.26];19(2):164-8. Available from: https://pharmed.zsmu.edu.ua/article/view/352352

Issue

Vol. 19 No. 2 (2026)

Section

Original research

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Copyright (c) 2026 Yu. V. Voronina-Tuzovskykh, V. M. Poletai, O. S. Bondar, V. O. Yanchenko, I. O. Savosta

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