The aim of the article is to investigate the local irritant effect on the skin and conjunctiva of the eyes of the amber-based oil complex TM IL SAV AMBER and to analyze its sensitizing activity in vivo.

Materials and methods. A series of chronic experiments of different durations (48 hours, 14 days and 4 weeks) were used to study the local irritant effect of the drug in vivo (on white sexually mature mice, 2 groups of animals, one of which was a control, n = 20 – method of skin applications; and on white-colored or white-spotted guinea pigs weighing 300–400 g, 2 groups of animals, one of which was a control, n = 10 – conjunctival test) and to study the sensitizing effect of the drug in vivo (on white-colored guinea pigs weighing 300–400 g, 4 groups of animals, two of which were control, n = 20 – method of repeated skin applications).

Results. Studies of the skin irritation effect of the drug in mice showed that dermal applications of the studied drug for 14 days did not cause signs of inflammation and damage – redness, edema, rashes and ulceration. The general behavior of the experimental animals (mobility, appetite, coat) did not differ from that of the control group. The conjunctival test did not reveal hyperemia and edema of the conjunctiva, sclera and lacrimal duct, and also did not cause immediate-type hypersensitivity reactions. The study of the sensitization properties of the studied drug using dermal applications did not reveal immediate-type and delayed-type hypersensitivity reactions in experimental animals in vivo.

Conclusions. The oil complex based on amber TM IL SAV AMBER did not affect general behavior, did not cause local irritation, and did not exhibit sensitizing activity in the studied animals in vivo.