The aim of the work is to establish morphological and anatomical diagnostic features of the underground organs of the Serratula coronata, harvested at the research sites in the Ternopil region in October 2025.

Materials and methods. The material for the research was the rhizome and roots of the S. coronata. The morphological and anatomical structure of the studied raw material was studied in accordance with the requirements of the monograph of the State Pharmacopoeia of Ukraine. Fresh and dried raw materials were used. When studying temporary preparations, the Delta Optical Genetic Pro optical device was used and a camera Delta Optical DLT-Cam Pro.

Results. According to external signs, the rhizome of Serratula coronata L. is short, woody and thick, from which numerous, thin, cylindrical branched, cord-like, adventitious roots depart. The color of the rhizome from the outside is dark brown, of the adventitious roots is dark brown, on the fracture it is whitish or yellow, the fracture surface is fibrous. The smell is weak and specific. The taste is bitter and astringent. The rhizome has a bundle-free type of structure. The covering tissue is the periderm, which includes the cortex. The cortex is absent in cross section; several rows of brown cells are located, partially torn; the cortex consists of several rows of tangentially elongated parenchyma cells, and near the inner edge narrow secretory ducts form a ring around the endoderm; the endoderm has a narrow Casparian strip; secondary phloem of the parenchyma with bundles of conductive tissue near the cambium; secondary xylem consists mainly of parenchyma, with vessels arranged in narrow radial rows; pith and starch are absent. Inulin is present in all parenchyma cells.

Conclusions. A morphological and anatomical study of the rhizome and adventitious roots of Serratula coronata L. was carried out and the main macroscopic and microscopic diagnostic features of underground organs were established.