Spectrophotometric determination of meloxicam in medicinal products
DOI:
https://doi.org/10.14739/2409-2932.2025.2.328975Keywords:
meloxicam, spectrophotometry, diazole red 2G, quantification, validation studyAbstract
Aim. To develop and validate methods for spectrophotometric determination of meloxicam in drugs by reaction with diazole red 2G.
Materials and methods. The following was used to perform the experiment: meloxicam substance pharmacopoeial purity; pharmaceutical drugs – “Meloxicam” tablets 0.0075 g, “Meloxicam” tablets 0.015 g, “Meloxicam-Teva” solution for injection 15 mg / 1.5 ml; as reagent – diazole red 2G of a reagent grade purity; solvents – 1,4-dioxane of a reagent grade purity and purified water; analytical equipment – spectrophotometer Specord-200 (Analytic Jena AG, Germany), scales laboratory electronic RADWAG XA 210.4Y, bath ultrasonic Sonorex Digitec DT100H, laboratory glassware of class A.
Results. New simple spectrophotometric methods for the quantitative determination of meloxicam in pharmaceuticals by reaction with diazole red G have been developed. The absorption maximum is recorded at 350 nm. The basic law of light absorption is followed in the concentration range of 1.2–2.4 mg / 100 ml. The limit of detection is 1.09 μg/ml, indicating a high sensitivity of the reaction. Linearity, limit of detection, limit of quantification, range of application, precision, accuracy and robustness were determined according to the requirements of the State Pharmacopoeia of Ukraine.
Conclusions. As a result of the study, spectrophotometric methods for the quantitative determination of meloxicam in medicinal products were developed and validated. The methods are simple, affordable and comply with the requirements of the State Pharmacopoeia of Ukraine, so they can be recommended for use in drug quality control laboratories.
References
Ghlichloo I, Gerriets V. Nonsteroidal Anti-Inflammatory Drugs (NSAIDs) [Updated 2023 May 1]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2025. Available from: https://www.ncbi.nlm.nih.gov/books/NBK547742/
Council of Europe. Meloxicam. In: European pharmacopoeia: Publ. in accordance with the convention on the elaboration of a European pharmacopoeia; 2016. p. 2994-6.
Derzhavna Farmakopeia Ukrainy [The State Pharmacopoeia of Ukraine]. 2nd ed. Kharkiv, (UA): State Enterprise Ukrainian Scientific Pharmacopoeial Center of Medicines Quality; 2015. Ukrainian.
United States Pharmacopoeial Convention. Meloxicam. In: United States pharmacopoeia: The National Formulary; 2019. p. 2767-9.
Ianiski FR, Laporta LV, Rubim AM, Luchese C. Validation of high performance liquid chromatography method for determination of meloxicam loaded PEGylated nanocapsules. Braz J Pharm Sci. 2015;51(4):823-32. doi: https://doi.org/10.1590/S1984-82502015000400008
Bandarkar FS, Vavia PR. A stability indicating HPLC method for the determination of meloxicam in bulk and commercial formulations. Trop J Pharm Res. 2009;8(3): 257-64. doi: https://doi.org/10.4314/tjpr.v8i3.44543
Çelik RS, Bayrak B, Kadioglu Y. Development and validation of HPLC-UV method for determination of meloxicam in tablet dosage formulation. Pharmata. 2023;3(3):59-63. doi: https://doi.org/10.5152/pharmata.2023.23008
Sahoo NK, Sahu M, Rao PS, Rani NS, Devi JI, Ghosh G. Validation of assay indicating method development of meloxicam in bulk and some of its tablet dosage forms by RP-HPLC. Springerplus. 2014;3:95. doi: https://doi.org/10.1186/2193-1801-3-95
Ahmad R, Hailat M, Zakaraya Z, Al Meanazel O, Abu Dayyih W. Development and Validation of an HPLC Method for the Determination of Meloxicam and Pantoprazole in a Combined Formulation. Analytica. 2022;3(2):161-77. doi: https://doi.org/10.3390/analytica3020012
Csifo E, Croitoru, Fülöp I, Muntean D-L. Development of a HPLC-UV method for determination of meloxicam in human plasma and pharmaceutical dosage forms. Acta Medica Marisiensis. 2014;60(4):142-5. doi: https://doi.org/10.2478/amma-2014-0031
Sawada H, Korenaga K, Kawamura N, Mizu H, Yamauchi H. A simple and easy method for determination of meloxicam in rat muscle and plasma. Chromatography. 2011;32(3):121-6. doi: https://doi.org/10.15583/jpchrom.2011.010
Sivarami Reddy BK. Development and Validation of HPLC Assay Method for Determination of Mesalamine in Bulk Drug and Tablet Formulation. International Journal of Scientific Engineering and Research. 2014;2(6):52-6. doi: https://doi.org/10.70729/j2013309
Basu SK, Sanchita MS. Spectrophotometric Estimation of Meloxicam in Dosage Forms. Asian journal of chemistry. 2009;21(7):5184-8. Available from: https://asianpubs.org/index.php/ajchem/article/view/18291/18240
Hasan SH, Othman NS, Surkhi KM. Development and Validation of a UV-Spectrophotometric Method for Determination of Meloxicam in Bulk and in TabletFormulations. International Journal of Pharma Sciences and Research. 2015;6(7):1040-5. Available from: https://www.ijpsr.info/docs/IJPSR15-06-07-010.pdf
Hasan SH, Othman NS, Surkhi KM. Spectrophotometric method for determination of meloxicam in pharmaceutical formulations using N-bromosuccinimide as an oxidant. International Journal of Pharma Sciences and Research. 2014;5(12):963-9. Available from: https://www.ijpsr.info/docs/IJPSR14-05-12-046.pdf
Dhandapani B, Eswara Murali S, Susrutha N, Swetha R, Rani S, Babu S, et al. Spectrophotometric estimation of meloxicam in bulk and its pharmaceutical formulations. International journal of pharma sciences and research. 2010;1(4):217-21.
Mandrescu MA, Pac AF, Dorneanu V. Spectrophotometric Determination of Meloxicam. Rev Chim. 2009;60(2):160-3.
El-Malla SF, Hamza AA, Elagamy SH. Simultaneous determination of meloxicam and bupivacaine via a novel modified dual wavelength method and an advanced chemometric approach. Sci Rep. 2024;14(1):1893. doi: https://doi.org/10.1038/s41598-024-51885-z
Ndueche CA, Ogbeide UM, Okeri HA. Colorimetric Determination of Meloxicam in Bulk and Tablet Dosage Forms. J Sci Pract Pharm. 2022;9(1):465-73. doi: https://doi.org/10.47227/jsppharm.v9i1.3
Donchenko A, Vasyuk S, Nahorna N. Extraction-free spectrophotometric determination of meloxicam using bromothymol blue. Ank Univ Eczaci Fak Derg. 2023;47(3): 752-60. doi: https://doi.org/10.33483/jfpau.1240900
Downloads
Additional Files
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 S. O. Vasyuk, A. S. Korzhova

This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.