Microscopic analysis of Valeriana stolonifera and Valeriana collina leaves

Authors

DOI:

https://doi.org/10.14739/2409-2932.2024.3.311562

Keywords:

Valeriana officinalis, Valeriana stolonifera, Valeriana collina, leaf anatomy, microscopic analysis

Abstract

Plants of the Valeriana species are distributed in various parts of the world, especially in Europe and Asia. A high content of polyphenolic compounds, including flavonoids and hydroxycinnamic acids with expressed biological activity, was previously identified within the herb of the studied Valeriana species. Morphological and anatomical data can be used in phylogenetics of species and genera to find out diagnostic and age-related characteristics of plants. This led us to microscopic studies of aerial organs of the above-mentioned Valeriana species.

The aim of the work is to conduct a comparative study of the diagnostic features within the morphological and anatomical structure of Valeriana stolonifera and Valeriana collina leaves.

Materials and methods. Both raw and dried plant material of V. stolonifera and V. collina was used for microscopic studies. Temporary micropreparations were made using generally accepted methods. The anatomical features of the raw material were examined using Carl ZEISS “AxioStar Plus” and “Primo Star”.

Results. The study identified key morphological and anatomical features of the species, which should be considered for the identification and standardization of promising medicinal plant materials and within the development of methods of analytical and regulatory documentation.

Conclusions. The key microscopic differences in the leaves of the studied species lie in the structure of the adaxial and abaxial surfaces of the leaf blade. The upper epidermis consists of large elongate cells with wavy walls. The cells of the lower epidermis are smaller and have more sinuous walls compared to the upper epidermis cells. The degree of wall sinuosity varies between species – V. collina is characterized by more sinuous cells in both the upper and lower epidermis compared to V. stolonifera. The absence of stomata on the upper epidermis is a common feature for both species.

Author Biographies

V. I. Kokitko, Zaporizhzhia State Medical and Pharmaceutical University, Ukraine

Graduate Student of the of the Department of Pharmacognosy, Pharmacology and Botany

V. M. Odyntsova, Zaporizhzhia State Medical and Pharmaceutical University, Ukraine

PhD, DSc, Professor of the Department of Pharmacognosy, Pharmacology and Botany

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Published

2024-11-08

How to Cite

1.
Kokitko VI, Odyntsova VM. Microscopic analysis of Valeriana stolonifera and Valeriana collina leaves. Current issues in pharmacy and medicine: science and practice [Internet]. 2024Nov.8 [cited 2026Jun.27];17(3):207-14. Available from: https://pharmed.zsmu.edu.ua/article/view/311562

Issue

Vol. 17 No. 3 (2024)

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Original research

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