Determination of riboflavin in dragee “Revit” by HPLC
DOI:
https://doi.org/10.14739/2409-2932.2022.2.258888Keywords:
riboflavin, dragee, vitamins, analysis, liquid chromatography, HPLCAbstract
The aim of the work is development of express, selective, reproducible methods for the determination of riboflavin by HPLC in a multivitamin preparation, dragee “Revit”.
Materials and methods. Degasser, binary pump, autosampler, thermostated column compartment, diode array detector. Chromatographic column Zorbax SB-C18, 30 mm × 4.6 mm, 1.8 µm. Samples were prepared using analytical electronic scales Kern ABT 100-5M, ultrasonic bath Ultrasonic XUBA 3, nylon ultrafilters 0.12 µm.
Results. 0.1 % formic acid was selected as a buffer solution in the eluent. The maximum values of logD were observed at intervals for riboflavin at pH from 2 to 4. Therefore, the maximum retention of the riboflavin was observed when using the eluent, рН which corresponds to this interval. The pH value for 0.1 % formic acid solution was 2.7. Therefore, such a buffer was chosen by us as part of the eluent. Acetonitrile was selected as the organic modifier. The optimal content of the compound was observed at 10 % acetonitrile.
Conclusions. The chromatographic behavior of riboflavin on the basis of chemical-analytical properties was studied. The optimal conditions for chromatographic determination were determined and a specific, express method for the determination of riboflavin in “Revit” dragee was developed. The technique on a real sample of “Revit” dragee was elaborated. The method was recommended for use in control and analytical and research laboratories.
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