The study of the pharmacological activity of thick extract from the leaves of Corylus avellana on the model of peripheral vascular thrombophlebitis in rabbit ear

Authors

  • L. M. Maloshtan National University of Pharmacy,
  • A. S. Kalenichenko National University of Pharmacy,

DOI:

https://doi.org/10.14739/2409-2932.2017.1.93443

Keywords:

plant extractis, Corylus, thrombophlebitis, fibrinolytic agents

Abstract

Creation of accessible domestic drug for the treatment and prevention of thrombotic venous damage, which would be able to adjust maximum range of disorders with minimal side effects, is actual direction of the pharmacology.

The aim of this work is to study the pharmacological activity of the extract from the leaves of Corylus avellana on the experimental model of thrombophlebitis in rabbit’s ears.

Materials and methods. Experimental thrombophlebitis was reproduced by overlaying the ligature on the rabbit ear border vein and introduction of Lugol solution in the vein cavity. This model allows to impaired blood flow as a result of stasis and inflammation of the vein wall, which is typical for the majority of thrombotic conditions in humans. Histological studies were carried out in accordance with standardized methods of light microscopy for the more detailed assessment of the pathological process intensity in the wall of the vein and its correction study by medication compared with the control group.

Results. It has been established that animals treated with the Corylus avellana leaves extract have damaged area of the ear by 23 % less in comparison with the control group on the first day of the experiment. On the 5th day of the experiment, the extract significantly reduced the inflammation area by 24 %, and on the 10th day – by 73 %. According to expression of the protective effect, the extract statistically significantly prevailed over comparison drug eskuvit throughout all experiment. The study of histological samples showed that the extract contributed to thrombolysis, prevented the destruction of the vascular wall, degenerative changes and reduced inflammation in the surrounding tissues and showed more demonstrative impact compared to the reference drug.

Conclusions. The obtained results are an evidence of the anti-inflammatory, vein-protective and thrombolytic action that have the superiority over eskuvit tablets. Thus, the results of this study allow to recommend the extract of Corylus avellana leaves as expressed vein-protective remedy. 

References

Abbas, A. K., Aster, J. C., & Kumar, V. (Eds.) (2012). Robbins Basic Pathology. Philadelphia, United States: Health Sciences Division.

Bogachev, V. Yu., Golovanova, O. V., Kuznecov, A. N., & Yershov, P. V. (2014). Khronicheskie zabolevaniya ven nizhnikh konechnostej: sovremennyj vzglyad na patogenez, lechenie i profilaktiku [Chronic diseases of the lower extremities: a modern view of the pathogenesis, treatment and prevention]. Farmateka, 20(293), 19–26. [in Russian].

Zhadko, S. V., & Syroiezhkina, O. V. (2014) Analiz asortymentu ta struktury spozhyvannia venotonizuiuchykh likarskykh zasobiv v Ukraini [Analysis of the range and patterns of consumption veinprotective remedy in Ukraine]. Profesiinyi menedzhment v suchasnykh umovakh rozvytku rynku. Proceedings 3кd of the Scientific and Practical Conference, (pp. 118–120). Kharkov. [in Ukrainian].

Maloshtan, L. M., Kalenichenko, G. S., & Dorovskyy, O. V. (2015). The study of the action of the thick extract from the leaves of corylus аvellana on the vascular tissue permeability. Klinichna pharmatsiia, 4, 25–29. [in Ukrainian].

Kalenichenko, A. S., Maloshtan, L. N., Shatalova, O. M., & Dorovskyy, О. V. (2015). Doslidzhennia mekhanizmu protyzapalnoi dii hustoho ekstraktu z lystia lishchyny zvychainoi [Study of the mechanism anti-inflammatory effect of the thick extract from the leaves of Corylus avellana] Ukrainskyi biofarmatsevtychnyi zhurnal, 41(6), 30–33. [in Ukrainian].

Kalenichenko, A. S., & Maloshtan, L. N. (2016). Skriningovoe issledovanie antikoagulyantnoj, membranostabiliziruyushchej i protivovospalitel´noj aktivnosti gustogo e´kstrakta iz list´ev leshchiny obyknovennoj [Screening study of anticoagulant, membrane stabilizing and anti-inflammatory activity of the thick extract from the leaves of corylus avellana]. Farmakolohіia ta lіkarska toksikolohіia, 2(48), 43–48. [in Ukrainian].

Maloshtan, L. M., Dolzhykova, O. V., & Batura, I. O. (2002). Informatsiinyi lyst Sposib modeliuvannia tromboflebitiv peryferychnykh sudyn [Information leter: The modeling method of peripheral vascular thrombophlebitis №25]. Kyiv: Ukrmedpatentprom. [in Ukrainian].

Rybolovlev, Yu. R., & Rybolovlev, R. S. (1979). Dozirovanie veshchestv dlya mlekopitayushchikh po konstantam biologicheskoj aktivnosti [Dosing for mammals substances constants of biological activity]. Doklady AN SSSR, 247(6), 1513–1516. [in Russian].

Merkulov, G. A. (1969). Kurs patologogistologicheskoj tekhniki [Course of pathohistological eguipment]. Moscow: Medicina, Leningradskoe otdelenie. [in Russian].

Gloviczki, P., Comerota, A. J, Dalsing, M. C., Eklof, B. G., Gillespie, D. L., Gloviczki, M. L., et al. (2011). The care of patients with varicose veins and associated chronic venous diseases: Clinical practice guidelines of the Society for Vascular Surgery and the American Venous Forum. Journal of vascular surgery, 53(16), 2–48. doi: 10.1016/j.jvs.2011.01.079.

Downloads

How to Cite

1.
Maloshtan LM, Kalenichenko AS. The study of the pharmacological activity of thick extract from the leaves of Corylus avellana on the model of peripheral vascular thrombophlebitis in rabbit ear. Current issues in pharmacy and medicine: science and practice [Internet]. 2017Feb.20 [cited 2024Apr.24];(1). Available from: http://pharmed.zsmu.edu.ua/article/view/93443

Issue

No. 1 (2017)

Section

Experimental and clinical pharmacology

License

Authors who publish with this journal agree to the following terms:

    1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.     Лицензия Creative Commons
    2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
    3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access)