Microscopic research on Plantago major L. and Plantago media L. in identification of medicinal plant-based raw material

Authors

  • T. V. Khortetska Zaporizhzhia State Medical University,
  • G. P. Smoilovska Zaporizhzhia State Medical University,

DOI:

https://doi.org/10.14739/2409-2932.2017.1.93439

Keywords:

Plantago major L., Plantago media L., microscopy

Abstract

Plant-based raw materials of generic species Plantago L. are widely used in world medicine as anti-inflammatory, vulnerary and anti-ulcerous agents. Pharmacologic efficiency is accounted by polysaccharides, aucubin, flavonoids, hydrocinnamic acids, vitamin A, amino-acids, etc. which are presented in the plant. Approximately 18 species of family Plantaginaceae Juss are grown up in Ukraine. But only Plantago major and Plantago lanceolate are officially recognized. Plantago media is near phylogenically to Plantago major, it has large plant base, similar composition and biologically active substances, but it isn’t used in Ukraine. Thus, comparative microscopic researches for two species of Plantago, revealing their diagnostic capacities for further practical using Plantago media in medicine are actual and topical.

The aim of our research was to determine general and specific diagnostic microscopic signs in plant-based raw material of Plantago major L. and Plantago media L.

Material and methods. Within microscopic study we have used leaves of Plantago major L. and Plantago media L. plants, picked during flowering in Ukraine. Fresh plant raw material has been fixed in mixture: glycerin, ethyl alcohol 96 %, purified water (1:1:1). The research has been done with using chloral hydrate according to methods recommended by State Pharmacopeia of Ukraine.

Results and discussion. While carrying out microscopic studies of plant raw material we turned our attention on structure of veins and cells in epidermis, availability, number and type of stoma, features for filaments and glandules, type of leaf lamina.

Conclusions. Isolateral type in structure of leaf lamina is the common microscopic diagnostic feature for both Plantago major L. and Plantago media L. The veins of both types are encircled by gross oval cells with starch inclusions. Related structure in lower epidermis of leaves, simple and glandulous filament are specific for both plants. Distinctions in anatomic structure of veins and stoma of studied species of Plantaginaceae Juss have been defined within microscopy. Presence of simple papilliform filament in Plantago media L. has been observed within microscopic study as well. Microscopic features obtained within our research allow to identify accurately plant raw material from morphologically related generic species Plantago major L. and Plantago media L.

References

Karpuk, V. V. (2011). Farmakognoziya [Pharmacognosy]. Minsk: BGU. [in Belarus].

Khortetska, T. V., Mazulin, O. V., Smoilovska, H. P., Mazulin, H. V., & Lohvin, P.A. (2013). Doslidzhennia optymalnykh umov sushinnia lystia podorozhnyka serednoho [Study of the optimal conditions of medium plantain leaves drying]. Zaporozhye medical journal, 2, 101–103 [in Ukrainian].

Yakovleva, L. V., Maciychuk, A. P., Kovalova, I. A., & Chorna, N. S. (2012). Vplyv novykh fitopreparativ na osnovi soku podorozhnyka na sekretsii shlunkovoho soku ta stan slyzovoi obolonky shlunka shchuriv za umov normy ta patolohii [The impact of new phytopreparations based on plantain juice on gastric juice secretion and the state of the gastric mucosa of rats in normal and pathological conditions]. Farmakolohiia ta likarska toksykolohiia, 5, 88–92 [in Ukrainian].

Derzhavne pidpryiemstvo «Ukrainskyi naukovyi farmakopeinyi tsentr yakosti likarskykh zasobiv» (2015). Derzhavna Farmakopeia Ukrainy [State Pharmacopoeia of Ukraine], (Vol. 1). Kharkov [in Ukrainian].

Kotova, E. E., Kotov, A. H., Vovk, O. H., & Hrunenko, Ya. A. (2010). Pytannia vvedennia do DFU natsionalnoi monohrafii «Podorozhnyka velykoho lystia» [The question of the introduction of national SPU monograph «Plantain leaves»]. Farmakom, 2, 5–13 [in Ukrainian].

Kovalov, V. M., Marchyshyn, S. M., Khvorost, O. P. et al. (2014). Praktykum z identyfikatsii likarskoi roslynnoi syrovyny [Workshop on identify catoin of medical plants]. V. M. Kovalov (Ed). Ternopil: TDMU. [in Ukrainian].

Velikanova, N. A., Slivkin, A. I., Gaponov, S. P., Podvigina, N. V., & Karlov, P. M. (2013). Izuchenie mikroskopicheskikh osobennostej list´ev podorozhnika bol´shogo, sobrannogo v gorode Voronezhe i ego okrestnost´yakh [Study Features microscopic lystev plantain more, sobrannoho in the city of Voronezh and ego okrestnostyah]. Vestnik Voronezhskogo gosudarstvennogo universiteta. Seriya: khimiya, biologiya, farmaciya, 2, 173–177 [in Russian].

How to Cite

1.
Khortetska TV, Smoilovska GP. Microscopic research on Plantago major L. and Plantago media L. in identification of medicinal plant-based raw material. Current issues in pharmacy and medicine: science and practice [Internet]. 2017Feb.20 [cited 2024Oct.16];(1). Available from: http://pharmed.zsmu.edu.ua/article/view/93439

Issue

Section

Pharmacognosy and chemistry of natural compounds