Comparative analysis of hydroxycinnamic acids of Artichoke cultivated in Ukraine and in France
Keywords:Cynara scolymus, hydroxycinnamic acids, caffeic acids, neochlorogenic acid, ferulic acid, chromatography, spectrophotometry
Purpose – comparative analysis of hydroxycinnamic acids present in the inflorescences of Artichoke (Cynarae flos) cultivated in Ukraine and in France.
Materials and methods. Identification of hydroxycinnamic acids was performed by thin layer chromatography of the ethanol extract of artichoke inflorescence. The research was carried out on TLC plates with a layer of silica gel of 5–40 μm. As the mobile phase 15% acetic acid was used. For the detection of the hydroxycinnamic acid, the chromatogram was handled with 10% solution of potassium hydroxide and diazotized sulfanilic acid, followed by heating the chromatograms in an oven for 3–5 minutes and visual analysis in the daylight. The quantitative content of hydroxycinnamic acids was determined by two methods. Determination was carried out using a spectrophotometric method calculated on the chlorogenic acid and absolutely dry raw material. According to the first method, the absorbance of water extract of the plant material was measured at a wavelength of 327 nm. As for the second procedure, the optical density of alcohol extract of artichoke inflorescence was measured at a wavelength of 525 nm after the reaction of complex formation of hydroxycinnamic acids with solutions of sodium molybdate and sodium nitrite.
Results. p-Coumaric, chlorogenic and neochlorogenic acids were identified in both samples of artichoke inflorescence using TLC method by comparison of Rf values, fluorescence under the UV-light before and after the ammonia vapors handle and by color of the spots after handle of chromatograms with ferric (III) chloride solution, 10% potassium hydroxide solution and diazitized sulfanilic acid. The content of hydroxycinnamic acids in artichoke inflorescence harvested in Ukraine and France was determined using the spectrophotometric method. Hydroxycinnamic acid content, determined by the method 1, was 1.64 % and 1.93 %, using method 2–1.66 % and 1.95 %, respectively.
Conclusions. The qualitative composition and quantitative content of hydroxycinnamic acids was determined in the artichoke inflorescence harvested in Ukraine and France. The results of the experiment allow to recommend the artichoke inflorescence of both domestic and foreign origin as the source of hydroxycinnamic acids.
(2015) Derzhavna Farmakopeia Ukrainy [State Pharmacopoeia of Ukraine]. (Vol. 1). Kharkiv. [in Ukrainian].
(2014) Derzhavna Farmakopeia Ukrainy [State Pharmacopoeia of Ukraine]. (Vol. 3). Kharkiv. [in Ukrainian].
Katsuba, I. K., Kyslychenko, V. S., & Novosel, O. M. (2011) Doslidzhennia fenolnykh spoluk lystia maty-i-machukhy [The study of phenolic compounds from the coltsfoot leaf]. Ukrainskyi medychnyi almanakh, 14(6), 92–94. [in Ukrainian].
K´osev, P. A. (2011) Lekarstvennye rasteniya: samyj polnyj spravochnik [Medicinal plants: the most complete directory]. Мoscow: E´ksmo-Press. [in Russian].
Men´schikova, E. B., Lankin, V. Z., & Kandalinceva, N. V. (2012) Fenol´nye antioksidanty v biologii i medicine. Stroenie, svojstva, mekhanizmy dejstviya [Phenolic antioxidants in biology and medicine. Structure, properties, mechanisms of action]. LAP LAMBERT Academic Publishing. [in Russian].
Horteckaya, T. V., Samojlovskaya, G. P., Mazulin, A. V., & Mazulin, G. V. (2014) Opredeleniye soderzhaniya gidroksikorichnykh kislot v list'yakh podorozhnika bol'shogo (Plantago major L.) i srednego (Plantago media L.) [Determination of hydroxycinnamic acids in leaves of Common plantain (Plantago major L.) and Hoary plantain (Plantago media L.)]. Himiya rastitel´nogo syr´ya, 2, 177–180. [in Russian].
Tymofieieva, S. V., & Zhuravel, I. O. (2016) Vyznachennia kilkisnoho vmistu hidroksykorychnykh kyslot u syrovyni kanny sadovoi (Canna x hybrid Hort.) [Determination of quantitative content of hydroxycinnamic acids in raw cannas garden (Canna x hybrid Hort.)]. Zbirnyk naukovykh prats spivrobitnykiv NMAPO imeni P. L. Shupyka, 26, 413–417. [in Ukrainian].
Makhatova, B., Datkhaev, U., Burda, N., Kislichenko, V., & Makhatova, A. (2015) Determination of phenolcarbonic acids in Verbascum songaricum raw materials. Journal of Chemical and Pharmaceutical Research., 7(3), 1787–1789.
How to Cite
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access)