The study of antimicrobial properties of immobilized fibrous N-chloro sulfonamides
DOI:
https://doi.org/10.14739/2409-2932.2016.3.77993Keywords:
N-fibrous Chloro Sulfonamides, Antimicrobial Properties, Dressings, Hypochlorous Acid, N-chlorotaurineAbstract
Aim. The determination of antibacterial and antifungal action of nonwoven form of N-chloro sulfonamides immobilized on a polypropylene fiber with a graft copolymer of styrene and divinylbenzene.
Materials and methods. The object of the study was a non-woven form of sodium N-chloro sulfonamides immobilized on a polypropylene fiber with a graft copolymer of styrene and divinylbenzene. Determination of antibacterial and antifungal action of the material was carried out by the "agar plate" at the meat peptone agar (МPA). As the test cultures of microorganisms we used: E.coli, S.aureus and C.albicans. Microbial load was 1х107 TEM/ml.
Results. The growing delay for such test microorganisms as E. coli strains, S.aureus and C.albicans fungi has been observed only for samples of material. Lack of microbial growth delay zones outside the samples occurs due to poor contact with the surface of the MPA. The method of "agar plate" has been changed to obtain reliable results of antibacterial and antifungal action of the material. The test material samples were placed in uncured MPA, the further experiment was carried out according to standard procedures of the "agar plates" method. Latency test microorganism growth around the sample material lies in the range 6,0 -7,0 mm – for E.coli; 16,0 -25,0 mm – for S.aureus; 4,0 -6,0 mm – for C.albicans. These results are explained by activation with the help of ammonium salts and amino acids, which release the active material of chlorine in case when the material contacts with the liquid nutrient medium. Evidently, this way the material will behave in contact with the skin of human (or animal). In contact with the dry skin covering the material will remain inactive, but in contact with the wound the release of active chlorine will occur within a specified period of time, whereby the material can protect the wound from infection.
Conclusions. Antibacterial and antifungal properties of non-woven form of sodium N-chloro sulfonamides immobilized on a polypropylene fiber with a graft copolymer of styrene and divinylbenzene are determined by the degree of contact with ammonium salt solutions and amino acid nutrient medium. While the surface contacts the growth inhibition of test cultures of microorganisms occurs only under the material. Introducing the material into a liquid medium greatly increases its activity. Thus, the material exhibits pronounced antimicrobial properties against the above test strains of microorganisms.
References
Tumanov, V. P., & German, G. S. (2000). Metodicheskoe rukovodstvo po lecheniyu ran [Methodological Guide for the treatment of wounds]. Moscow: izd-vo Paul' Khartmann. [in Russian].
Sun, Yu. Yu., & Luo, J. (patentee) (2006). N-Halamine vinyl compounds and their polumeric biocides: pat. 7084208 SSHA: MPK A61L15/46, D06M15/423.
Hsu, B., Hagerman, S., Jamieson, K., Castleberry, S., Wang, W., Holer, E., et al. (2015). Multifunctiohal self-assembled film for hemostat and sustained anti-infective deliveri. ACS Biomater. Sci. Eng., 1(3), 148–156. doi: 10.1021/ab500050m.
Soldatov, V. S. (2008). Syntheses and main properties of Fiban fibrous ion echangers. Solvent extraction and ion exchange, 26, 457–513.
Burmistrov, K. S., Murashevych, B. V., Toropіn, V. M., & Toropіn M. V. (2016). Zaiava №u 2016 05241 Ukraina: MPK: A61K31/00, A01P1/00, C08F8/38 Novyi polimernyi material z immobilizovanym aktyvnym khlorom, shcho proiavliaie antymikrobni vlastyvosti [Statement №u 2016 05241 Ukraine, IPC: A61K31/00, A01P1/00, C08F8/38 New polymeris material with immobilized active chlorine that exhibits an antimicrobial properties]. [in Ukrainian].
Gottardi, W., Debabov D., & Nagl M. (2013). N-Chloramines, a promising class of well-tolerated topical anti-infectives. Antimicrobal Agents and Chematerapy, 3(57), 1107–1114. doi: 10.1128/AAC.02132-12.
(1998) Metodicheskie ukazaniya «Metody ispytanij dezinfekcionnykh sredstv, dlya ocenki ikh bezopasnosti i e´ffektivnosti» [Disinfectants test methods for evaluation of safety and efficacy]. Moscow. [in Russian].
Derzhavne pidpryiemstvo «Ukrainskyi naukovyi farmakopeinyi tsentr yakosti likarskykh zasobiv». (2011). Derzhavna Farmakopeia Ukrainy. Kharkiv. [in Ukrainian].
Panasenko, O. M., Gorudko, I. V., & Sokolov, A. V. (2013). Hlornovatistaya kislota kak predshestvennik svobodnykh radikalov v zhivykh sistemakh [Hypochlorous acid as a precursor of free radicals in living systems]. Uspekhi biologicheskoj khimii, 53, 195–244. [in Russian].
Ivanov, S. V., Yudina, S. M., Chuharev, A. M., Bondarev G. A., Dolzhenko, S. N., Gaponov, A. M., et al. (1999). Lechenie razlitogo peritonita [Treatment of peritonitis]. Metodicheskie razrabotki dlya obshchikh khirurgov, studentov medvuzov starshikh kursov. Kursk. [in Russian].
Downloads
How to Cite
Issue
Section
License
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access)
