The development of quantitative determination method of organic acids in complex poly herbal extraction

Authors

  • I. L. Dyachok Lviv National Medical University naimed after Danylo Halytskyj,
  • O. R. Pinyashko Lviv National Medical University naimed after Danylo Halytskyj,

DOI:

https://doi.org/10.14739/2409-2932.2016.2.71129

Keywords:

Izovaleriс Acid, Organic Acids, Plant Extracts, Conductometry

Abstract

Aim. The development of sensible, economical and expressive method of quantitative determination of organic acids in complex poly herbal extraction counted on izovaleric acid with the use of digital technologies.

Materials and methods. Model complex poly herbal extraction of sedative action was chosen as a research object. Extraction is composed of these medical plants: Valeriana officinalis L., Crataégus, Melissa officinalis L., Hypericum, Mentha piperita L., Húmulus lúpulus, Viburnum.

Based on chemical composition of plant components, we consider that main pharmacologically active compounds, which can be found in complex poly herbal extraction are: polyphenolic substances (flavonoids), which are contained in Crataégus, Viburnum, Hypericum, Mentha piperita L., Húmulus lúpulus; also organic acids, including izovaleric acid, which are contained in Valeriana officinalis L., Mentha piperita L., Melissa officinalis L., Viburnum; the aminoacid are contained in Valeriana officinalis L.

For the determination of organic acids content in low concentration we applied instrumental method of analysis, namely conductometry titration which consisted in the dependences of water solution conductivity of complex poly herbal extraction on composition of organic acids.

Result. The got analytical dependences, which describes tangent lines to the conductometry curve before and after the point of equivalence, allow to determine the volume of solution expended on titration and carry out procedure of quantitative determination of organic acids in the digital mode.

Conclusion. The proposed method enables to determine the point of equivalence and carry out quantitative determination of organic acids counted on izovaleric acid with the use of digital technologies, that allows to computerize the method on the whole.

References

Trineeva, O. V., Safonova, I. I., Safonova, E. F., & Slivkin, A. I. (2013). Identifikaciya organicheskikh kislot metodom TSH v izvlecheniyakh iz rastitel'nykh ob′′ektov [Identification of organic acids by the TLC method in extraction from vegetable objects]. Sorbcionnye i khromatograficheskie process, 896–901 [in Russian].

Lebeda, A. F., Dzhurenko, N. I., Isajkina, A. P., & Sobko, V. G. (2010). Lekarstvennye rasteniya. Samaya polnaya e′nciklopediya [Medicinal plants. The most complete encyclopedia]. Moscow: AST-PRESS KNIGA. [in Russian].

Kyslychenko, V. S., Zhuravel, I. O., Bukharina, O. V., et al. (2009). Syrovynni dzherela produktiv biotekhnolohii ta yikh analiz [Raw material sources of biotechnology products and it’s analysis]. Kharkiv: NFaU : Zoloti storinky. [in Ukranian].

Kovalov, V. M., Marchyshyn, S. M., Khvorost, O. P., et al. (2014). Praktykum z identyfikatsii likarskoi roslynnoi syrovyny [Workshop on identifi cation of medicinal plants]. Ternopil : TDMU. [in Ukranian].

Georgievskij, V. P. (Ed). (2011). Analiticheskaya khimiya v sozdanii, standartizacii i kontrole kachestva lekarstvenny`kh sredstv [Analytical chemistry in the creation, standardization and quality control of medicines]. (Vol. 2). Kharkiv: NTMT. [in Ukrainian].

Derzh. p-vo «Naukovo-ekspertnyi farmakopeinyi tsentr» (2009) Derzhavna Farmakopeia Ukrainy [State Pharmacopoeia of Ukraine], (Dodatok 3). Kharkiv [in Ukrainian].

Kortikov, V. N., & Kortikov, A. V. (2002) Polnaya e`nciklopediya lekarstvennykh rastenij [Complete Encyclopedia of Medicinal Plants]. Rostov na Donu: Prof-Press. [in Russian].

K`osev, P. A. (2011) Lekarstvennye rasteniya: samyj polnyj spravochnik [Medicinal plants: the most complete directory]. Moscow: E`ksmo-Press. [in Russian].

Derzh. p-vo «Naukovo-ekspertnyi farmakopeinyi tsentr» (2001) Statystychnyi analiz rezultativ khimichnoho eksperymentu [Statistical analysis of chemical experiment]. Derzhavna Farmakopeia Ukrainy, (Dodatok 1 – 2004). Kharkiv [in Ukrainian].

Kozyra, M., & Glowniak, K. (2013) Phenolic acids in extracts obtained from the fl owering herbs of Cirsium vulgare (savi) Ten. growing in Poland. Acta Societatis Botanicorum Poloniae, 82(4), 325–329. doi: 10.5586/asbp.2013.039.

How to Cite

1.
Dyachok IL, Pinyashko OR. The development of quantitative determination method of organic acids in complex poly herbal extraction. Current issues in pharmacy and medicine: science and practice [Internet]. 2016Jun.10 [cited 2024Jul.21];(2). Available from: http://pharmed.zsmu.edu.ua/article/view/71129

Issue

Section

Pharmacognosy and chemistry of natural compounds