Determination of biologically active compounds in lipophilic extracts of pumpkin using thin layer chromatography

Authors

  • E. A. Degtyaryovа
  • V. A. Grudko
  • L. I. Vishnevskaya
  • E. I. Bisaga

DOI:

https://doi.org/10.14739/2409-2932.2015.2.45156

Keywords:

Cucurbita, Biologically Active Connections, Carotenoids, Phytosterols, Thin Layer Chromatography

Abstract

Aim. Carotenoids are a common and important class of natural fat-soluble pigments. The prevention of free radicals formation is the carotenoid’s primary effect. One of the tasks while creating medicines which contain carotenoids is the identification of them in plant objects.

Methods and results. Our purpose is the determination of biologically active compounds in lipophilic extracts of pumpkin using thin layer chromatography. The article presents the experimental study of solvent systems. Although, different developers which are recommended in literature have been chosen and investigated, As a result biologically active compounds of the lipophilic character have been identified, β-carotene has been identified, solvents and reagents for the identification of β-carotene have been selected.

Conclusion. This method can be included to the quality control methods to determine β-carotene in the lipophilic pumpkins extracts.

References

Heis F. (1999).Osnovy tonkosloinoy khromatografii. T. 1, M., Mir, 405 с.

Dayronas Zh. V., Pshukova I. V. (2010). Izuchenie sostava lipofilnoy fraktsii listev orekha gretskoho, proizrastaiushcheho v kavkazskikh mineralnykh vodakh // Khimiya rastitelnoho syrya, 4, 91-93.

Evheneva I.I. (1999). Planarnaia khromatohrafiia i analiz orhanicheskykh veshchestv // Sorosovskiy obrazovatelnyi zhurnal, 11, 50-55.

Keits M. (1975).Tekhnika lipidolohii. M., 324.

Smirnova T.I., Malakhaev E.D., Baranovskiy I.N. (2012).Vozdeystvie etilendiamindiiantarnoy kisloty i ee kompleksa s tsinkom na soderzhanie karotinoidov v rasteniiakh // Vestnik TvGu. Seriia «Khimiia»,13, 61-65.

Sisieva M.A., Khabibrakhmanova V.R., Hamaiurova V.S. (2008). Issledovanie zolia vodnikh izvlecheniy chagi. XI. Lipidi vodnoho izvlecheniy chagi. // Khimiya rastitelnoho syrya, 3, 119-122.

Ulianovskiy N.V., Kosiakov D.S., Bogolitsin K.G. (2012).Razraboyka ekspressnikh metodov analiticheskoy ekstraktsii karotinoidov iz rastitelnoho syrya. // Khimiya rastitelnoho syrya, 4, 147-152.

Checheta O.V., Safonova E.F., Slivkin A.I. (2008). Metodika opredeleniia karotinoidov metodom khromatografii v tonkom sloe sorbenta // Sorbtsionnie i khromatograficheskie protsesi, 8 (2), 320-326.

Shapovalova E.N., Pirogov A.V. (2007). Khromatograficheskie metodi analiza. / Metodicheskoe posobie dlia spetsialnogo kursa. Moskva, 203.

Shobha Borhade (2012).Extraction and characterisation of pumpkin (Cucurbita Mixta) seed oil // Life sciences Leaflets, 7, 45-49.

Mi Young K., Eun Jin K., Young-Nam K. et al. (2012) // Nutrition Research and Practice (Nutr Res Pract), 6 (1), 21-27.

Okoro I.A., Okoro C.A. (2007). Isolation and identificstion of lipids from different parts of fluted pumpkin seeds // Research Jurnal of Applied Sciences, 2(1), 114-116.

Hui Ni, Guo-qing. Hui Ruan at al. (2005) Application of derivative ratio spectrophotometry for determination of β-carotene and astaxanthin from Phaffia rhodozyma extract. // J Zhejiang Univ Sci B., № 6(6), 514-522.

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How to Cite

1.
Degtyaryovа EA, Grudko VA, Vishnevskaya LI, Bisaga EI. Determination of biologically active compounds in lipophilic extracts of pumpkin using thin layer chromatography. Current issues in pharmacy and medicine: science and practice [Internet]. 2015Jul.14 [cited 2024Nov.15];(2). Available from: http://pharmed.zsmu.edu.ua/article/view/45156

Issue

No. 2 (2015)

Section

Pharmacognosy and chemistry of natural compounds

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