Development and validation of the methods of captopril spectrophotometric determination in blood by the reaction with the Ellman reagent
Keywords:Validation Studies, Analitical Chemistry Techniques, Captopril, Ellman’s reagent, Spectrophotometry
Aim. To rationalize quantitative determinations in forensic and toxicological analysis, the possibility to use the method of standard for analytes quantitative determination in biological fluids has been studied.
Methods and results. The series of spectrophotometric methods of captopril quantitative determination in blood based on reaction with the Ellman reagent has been developed using amphiphylic solvents (isopropanol, acetonitrile, methanol) in condition of aqueous phase saturation by ammonium sulphate. Validation of the developed methods in the variant of the method of standard has been carried out, conformityof the obtained values of validation parameters to the acceptability criteria has been shown.
Conclusion. It has been set that acetonitrile application in the acid medium (рН=2) is optimal.
Tiwari, G. (2010) Bioanalytical method validation: an updated review. Pharm. Methods, 1(1), 25–38. doi: 10.4103/2229-4708.72226.
Moffat, A. C., Osselton, M. D., & Widdop B. (Eds.) (2011) Clarke’s analysis of drugs and poisons in pharmaceuticals, body fluids and postmortem material. London: Pharmaceutical Press.
(2009) Guidance for the Validation of Analytical Methodology and Calibration of Equipment used for Testing of Illicit Drugs in Seized Materials and Biological Specimens. United Nations Office on Drugs and Crime, Laboratory and Scientific Section. New York, United Nations.
(2013) Standard Practices for Method Validation in Forensic Toxicology. Scientific Working Group for Forensic Toxicology (SWGTOX).
Bolotov, V. V., Shovkovaya, Z. V., Merzlikin, S. I., Klimenko, L. Yu. (2007) Spektrofotometrychne vyznachennia kaptoprylu za dopomohoiu fotometrychnoi reaktsii z reaktyvom Ellmana [Spectrophotometric determination of captopril by the help of photometric reaction with the Ellman reagent]. Zhurnal orhanichnoi ta farmatsevtychnoi khimii, 4(20), 63–66. [in Ukrainian].
Klimenko, L. Yu. (2014) Razrabotka podkhodov k opredeleniyu linejnosti, pravil'nosti i precizionnosti UF-spektrofotometricheskikh metodik kolichestvennogo opredeleniya metodom standarta v sudebno-toksikologicheskom analize [Development of approaches to determination of linearity, accuracy and precision of UV-spectrophotometric methods of quantitative determination by the method of standard in forensic and toxicological analysis]. Farmaciya Kazakhstana, 4, 31–35. [in Kazakhstan].
Klimenko, L. Yu., Petyunin, G. P., Kostina, T. A. (2013) Podkhody k opredeleniyu specifichnosti/selektivnosti pri validacii UF-spektrofotometricheskikh metodik kolichestvennogo opredeleniya v sudebno-toksikologicheskom analize [Approaches to determination of specificity/selectivity when validating UV-spectrophotometric methods of quantitative determination in forensic and toxicological analysis]. Farmaciya Kazakhstana, 8, 53–56. [in Russian].
Klimenko, L. Yu., Trut, S. M., Petyunin, G. P., & Ivanchuk, I. M. (2013)Validation of UV-spectrophotometric methods of quantitative determination in forensic and toxicological analysis: recovery. Farmaciya Kazakhstana, 12, 42–48. [in Kazakhstan].
Shovkova, Z. V., Bolotov, V. V., Merzlikin, S. I., & Klimenko, L. Yu. (2010) Rozrobka metodyk izoliuvannia kaptoprylu iz biolohichnykh ridyn orhanizmu [Development of captoprie isolation methods from organism biological liquids]. Zaporozhskij medicinskij zhurnal, 6, 84–87. [in Ukrainian].
Gerasimov, D. A. (2014) Khimiko-toksikologicheskoe issledovanie nimesulida i blizkikh po structure soedinenij (Dis…kand. farm. nauk). [Chemical and toxicological research of nimesulide and similar compounds by structure]. Dr. farm. sci. diss.]. Kursk. [in Russian].
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