Development and validation of the methods of captopril spectrophotometric determination in blood by the reaction with the Ellman reagent

Authors

  • L. Yu. Klimenko
  • Z. V. Showkova
  • O. Ye. Mykytenko
  • A. G. Kaplaushenko

DOI:

https://doi.org/10.14739/2409-2932.2014.3.29952

Keywords:

Validation Studies, Analitical Chemistry Techniques, Captopril, Ellman’s reagent, Spectrophotometry

Abstract

Aim. To rationalize quantitative determinations in forensic and toxicological analysis, the possibility to use the method of standard for analytes quantitative determination in biological fluids has been studied.

Methods and results. The series of spectrophotometric methods of captopril quantitative determination in blood based on reaction with the Ellman reagent has been developed using amphiphylic solvents (isopropanol, acetonitrile, methanol) in condition of aqueous phase saturation by ammonium sulphate. Validation of the developed methods in the variant of the method of standard has been carried out, conformityof the obtained values of validation parameters to the acceptability criteria has been shown.

Conclusion. It has been set that acetonitrile application in the acid medium (рН=2) is optimal.

 

References

Tiwari, G. (2010) Bioanalytical method validation: an updated review. Pharm. Methods, 1(1), 25–38. doi: 10.4103/2229-4708.72226.

Moffat, A. C., Osselton, M. D., & Widdop B. (Eds.) (2011) Clarke’s analysis of drugs and poisons in pharmaceuticals, body fluids and postmortem material. London: Pharmaceutical Press.

(2009) Guidance for the Validation of Analytical Methodology and Calibration of Equipment used for Testing of Illicit Drugs in Seized Materials and Biological Specimens. United Nations Office on Drugs and Crime, Laboratory and Scientific Section. New York, United Nations.

(2013) Standard Practices for Method Validation in Forensic Toxicology. Scientific Working Group for Forensic Toxicology (SWGTOX).

Bolotov, V. V., Shovkovaya, Z. V., Merzlikin, S. I., Klimenko, L. Yu. (2007) Spektrofotometrychne vyznachennia kaptoprylu za dopomohoiu fotometrychnoi reaktsii z reaktyvom Ellmana [Spectrophotometric determination of captopril by the help of photometric reaction with the Ellman reagent]. Zhurnal orhanichnoi ta farmatsevtychnoi khimii, 4(20), 63–66. [in Ukrainian].

Klimenko, L. Yu. (2014) Razrabotka podkhodov k opredeleniyu linejnosti, pravil'nosti i precizionnosti UF-spektrofotometricheskikh metodik kolichestvennogo opredeleniya metodom standarta v sudebno-toksikologicheskom analize [Development of approaches to determination of linearity, accuracy and precision of UV-spectrophotometric methods of quantitative determination by the method of standard in forensic and toxicological analysis]. Farmaciya Kazakhstana, 4, 31–35. [in Kazakhstan].

Klimenko, L. Yu., Petyunin, G. P., Kostina, T. A. (2013) Podkhody k opredeleniyu specifichnosti/selektivnosti pri validacii UF-spektrofotometricheskikh metodik kolichestvennogo opredeleniya v sudebno-toksikologicheskom analize [Approaches to determination of specificity/selectivity when validating UV-spectrophotometric methods of quantitative determination in forensic and toxicological analysis]. Farmaciya Kazakhstana, 8, 53–56. [in Russian].

Klimenko, L. Yu., Trut, S. M., Petyunin, G. P., & Ivanchuk, I. M. (2013)Validation of UV-spectrophotometric methods of quantitative determination in forensic and toxicological analysis: recovery. Farmaciya Kazakhstana, 12, 42–48. [in Kazakhstan].

Shovkova, Z. V., Bolotov, V. V., Merzlikin, S. I., & Klimenko, L. Yu. (2010) Rozrobka metodyk izoliuvannia kaptoprylu iz biolohichnykh ridyn orhanizmu [Development of captoprie isolation methods from organism biological liquids]. Zaporozhskij medicinskij zhurnal, 6, 84–87. [in Ukrainian].

Gerasimov, D. A. (2014) Khimiko-toksikologicheskoe issledovanie nimesulida i blizkikh po structure soedinenij (Dis…kand. farm. nauk). [Chemical and toxicological research of nimesulide and similar compounds by structure]. Dr. farm. sci. diss.]. Kursk. [in Russian].

Downloads

How to Cite

1.
Klimenko LY, Showkova ZV, Mykytenko OY, Kaplaushenko AG. Development and validation of the methods of captopril spectrophotometric determination in blood by the reaction with the Ellman reagent. Current issues in pharmacy and medicine: science and practice [Internet]. 2014Nov.12 [cited 2024Nov.22];(3). Available from: http://pharmed.zsmu.edu.ua/article/view/29952

Issue

No. 3 (2014)

Section

Analysis and standardization of biologycally active substances and dosage forms

License

Authors who publish with this journal agree to the following terms:

    1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.     Лицензия Creative Commons
    2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
    3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access)