Development and validation of the methods of captopril spectrophotometric determination in blood by the reaction with the Ellman reagent

Authors

  • L. Yu. Klimenko
  • Z. V. Showkova
  • O. Ye. Mykytenko
  • A. G. Kaplaushenko

DOI:

https://doi.org/10.14739/2409-2932.2014.3.29952

Keywords:

Validation Studies, Analitical Chemistry Techniques, Captopril, Ellman’s reagent, Spectrophotometry

Abstract

Aim. To rationalize quantitative determinations in forensic and toxicological analysis, the possibility to use the method of standard for analytes quantitative determination in biological fluids has been studied.

Methods and results. The series of spectrophotometric methods of captopril quantitative determination in blood based on reaction with the Ellman reagent has been developed using amphiphylic solvents (isopropanol, acetonitrile, methanol) in condition of aqueous phase saturation by ammonium sulphate. Validation of the developed methods in the variant of the method of standard has been carried out, conformityof the obtained values of validation parameters to the acceptability criteria has been shown.

Conclusion. It has been set that acetonitrile application in the acid medium (рН=2) is optimal.

 

References

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Klimenko, L. Yu., Petyunin, G. P., Kostina, T. A. (2013) Podkhody k opredeleniyu specifichnosti/selektivnosti pri validacii UF-spektrofotometricheskikh metodik kolichestvennogo opredeleniya v sudebno-toksikologicheskom analize [Approaches to determination of specificity/selectivity when validating UV-spectrophotometric methods of quantitative determination in forensic and toxicological analysis]. Farmaciya Kazakhstana, 8, 53–56. [in Russian].

Klimenko, L. Yu., Trut, S. M., Petyunin, G. P., & Ivanchuk, I. M. (2013)Validation of UV-spectrophotometric methods of quantitative determination in forensic and toxicological analysis: recovery. Farmaciya Kazakhstana, 12, 42–48. [in Kazakhstan].

Shovkova, Z. V., Bolotov, V. V., Merzlikin, S. I., & Klimenko, L. Yu. (2010) Rozrobka metodyk izoliuvannia kaptoprylu iz biolohichnykh ridyn orhanizmu [Development of captoprie isolation methods from organism biological liquids]. Zaporozhskij medicinskij zhurnal, 6, 84–87. [in Ukrainian].

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How to Cite

1.
Klimenko LY, Showkova ZV, Mykytenko OY, Kaplaushenko AG. Development and validation of the methods of captopril spectrophotometric determination in blood by the reaction with the Ellman reagent. Current issues in pharmacy and medicine: science and practice [Internet]. 2014Nov.12 [cited 2024Jul.16];(3). Available from: http://pharmed.zsmu.edu.ua/article/view/29952

Issue

No. 3 (2014)

Section

Analysis and standardization of biologycally active substances and dosage forms

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