Investigation of antioxidant interaction between ascorbic acid and rutin in medicine “Ascorutin”

Authors

DOI:

https://doi.org/10.14739/2409-2932.2024.1.295418

Keywords:

antioxidant activity, interaction, rutin, ascorbic acid, antagonism

Abstract

Phytomedicines are combined pharmaceuticals containing various groups of phenolic compounds. It is known that phenolic compounds are powerful antioxidants, but their level of antioxidant activity during interactions has not been previously studied.

The aim of the work was to investigate interaction between ascorbic acid and rutin in medicine “Ascorutin”.

Materials and methods. The objects of the study were one series of tablets “Ascorutin,” rutin, and ascorbic acid. The level of antioxidant activity was measured by the potentiometric method.

Results. The level of antioxidant activity of model solutions of rutin was 265.51 ± 1.00 mmol-eq./L, ascorbic acid – 99.00 ± 1.00 mmol-eq./L, a combination of rutin and ascorbic acid – 255.60 ± 1.00 mmol-eq./L, and medicine “Ascorutin” – 256.52 ± 1.00 mmol-eq./L. An experimental value of the level of antioxidant activity of “Ascorutin”, the combination of rutin and ascorbic acid was 30 % less than theoretical values.

Conclusions. The antioxidant interaction of ascorbic acid and rutin in “Ascorutin” medicine has been studied. It has been established that the combination of rutin and ascorbic acid has an antagonistic effect. The findings of this research will serve as a stimulus for exploring the interaction of bioactive substances and selecting their optimal combinations for the development of pharmaceuticals, special food products, food, and cosmetic products.

Author Biographies

O. Yu. Maslov, National University of Pharmacy, Kharkiv, Ukraine

PhD, Assistant of the Department of General Chemistry

M. A. Komisarenko, National University of Pharmacy, Kharkiv, Ukraine

PhD, Assistant of the Department of Pharmacognosy and Nutriciology

S. V. Kolisnyk, National University of Pharmacy, Kharkiv, Ukraine

PhD, DSc, Professor, Head of the Department of General Chemistry

M. Yu. Golik, National University of Pharmacy, Kharkiv, Ukraine

PhD, DSc, Professor of the Department of General Chemistry

References

Maslov OY, Komisarenko MA, Kolisnyk SV, Golik MY, Tsapko YO, Akhmedov EY. Determination of the extraction frequency of green tea leaves by the antioxidant method. J Org Pharm Chem. 2022;20(1):28-34. doi: https://doi.org/10.24959/ophcj.22.252320

Maslov OY, Komisarenko MA, Ponomarenko SV, Kolisnyk SV, Osolodchenko TP, Kostina TA, et al. Antioxidant, antimicrobial and antifungal activity of the obtained "Cachisept" tablets for resorption in the oral cavity for the treatment and prevention of dental caries. Current issues in pharmacy and medicine: science and practice. 2023;16(3):213-6. doi: https://doi.org/10.14739/2409-2932.2023.3.285425

Maslov OY, Komisarenko MA, Kolisnyk SV, Antonenko OV, Kolisnyk OV, Kostina TA. The study of the qualitative composition and the quantitative content of phenolic compounds in dietary supplements with lingonberry. J Org Pharm Chem. 2021;19(4):40-6. doi: https://doi.org/10.24959/ophcj.21.243782

Maslov O, Komisarenko M, Kolisnyk S, Kostina T, Golik M, Moroz V, et al. Investigation of the extraction dynamic of the biologically active substances of the raspberry (Rubus idaeus L.) shoots. Curr Issues Pharm Med Sci. 2023;36(4):194-8. doi: https://doi.org/10.2478/cipms-2023-0034

Maslov OY, Komisarenko MA, Golik MY, Kolisnyk SV, Altukhov AA, Baiurka SV, et al. Study of total antioxidant capacity of red raspberry (Rubus idaeous L.) shoots. Rev Vitae. 2023;30(1). doi: https://doi.org/10.17533/udea.vitae.v30n1a351486

Maslov O, Kolisnyk S, Komisarenko M, Golik M, Antonenko O. Study of chemical composition and antioxidant activity of tincture, infusion of green tea leaves. Fitoterapiia. Chasopys. 2022;(1):48-52. doi: https://doi.org/10.33617/2522-9680-2022-1-48

Maslov OY, Kolisnyk SV, Komisarenko MA, Kostina TA, Dynnyk KV. Development the composition and technology for obtaining a dietary supplement "Cachinol" with the antioxidant activity in the form of granules used in the polycystic ovary syndrome. News of Pharmacy. 2022;103(1):42-7. doi: https://doi.org/10.24959/nphj.22.77

Maslov OY, Kolisnyk SV, Hrechana OV, Serbin AH. Study of the qualitative composition and quantitative content of some groups of BAS in dietary supplements with green tea leaf extract. Zaporozhye medical journal. 2021;23(1):132-7. doi: https://doi.org/10.14739/2310-1210.2021.1.224932

Maslov OY. [Phytochemical study and standardization medicines with antioxidant activity from green tea leaves] [dissertation]. Kharkiv, Ukraine: National University of Pharmacy; 2022. Available from: https://nrat.ukrintei.ua/en/searchdoc/0823U100107/

Maslov OY, Kolisnyk SV, Komisarenko MA, Altukhov AA, Dynnyk KV, Stepanenko VI. Study and evaluation antioxidant activity of dietary supplements with green tea extract. Current issues in pharmacy and medicine: science and practice. 2021;14(2):215-9. doi: https://doi.org/10.14739/2409-2932.2021.2.233306

Gęgotek A, Skrzydlewska E. Antioxidative and Anti-Inflammatory Activity of Ascorbic Acid. Antioxidants. 2022;11(10):1993. doi: https://doi.org/10.3390/antiox11101993

Yang J, Guo J, Yuan J. In vitro antioxidant properties of rutin. LWT - Food Science and Technology. 2008;41(6):1060-6. doi: https://doi.org/10.1016/j.lwt.2007.06.010

Maslov OY, Kolisnyk SV, Komisarenko MA, Akhmedov EY, Koval AO, Kostina TA, et al. Metrological Characteristics of the Potentiometric Assay Developed for Determining the Antioxidant Activity of Ascorbic Acid. J Org Pharm Chem. 2023;21(3):31-7. doi: https://doi.org/10.24959/ophcj.23.276394

Maslov O, Komisarenko M, Ponomarenko S, Horopashna D, Osolodchenko T, Kolisnyk S, et al. Investigation the influence of biologically active compounds on the antioxidant, antibacterial and anti-inflammatory activities of red raspberry (Rubus idaeous l.) leaf extract. Curr Issues Pharm Med Sci. 2022;35(4):229-35. doi: https://doi.org/10.2478/cipms-2022-0040

Maslov O, Kolisnyk S, Komisarenko M, Komisarenko A, Osolodchenko T, Ponomarenko S. In vitro antioxidant and antibacterial activities of green tea leaves (Camella sinensis l.) liquid extracts. Annals of Mechnikov Institute. 2022;(2):64-7. doi: https://doi.org/10.5281/zenodo.6634846

Maslov OY, Kolisnyk SV, Ponomarenko SV, Ahmedov EY, Shovkova ZV. The study of the effect of ethyl alcohol concentrations on the antioxidant activity of ascorbic acid solutions. J Org Pharm Chem. 2021;19(2):44-7. doi: https://doi.org/10.24959/ophcj.21.231947

Flaten MA. Drug effects: agonistic and antagonistic processes. Scand J Psychol. 2009;50(6):652-9. doi: https://doi.org/10.1111/j.1467-9450.2009.00776.x

Matsui T, Kitagawa Y, Okumura M, Shigeta Y. Accurate standard hydrogen electrode potential and applications to the redox potentials of vitamin C and NAD/NADH. J Phys Chem A. 2015;119(2):369-76. doi: https://doi.org/10.1021/jp508308y

Namazian M, Zare HR, Coote ML. Determination of the absolute redox potential of Rutin: experimental and theoretical studies. Biophys Chem. 2008;132(1):64-8. doi: https://doi.org/10.1016/j.bpc.2007.10.010

Downloads

Published

2024-02-23

How to Cite

1.
Maslov OY, Komisarenko MA, Kolisnyk SV, Golik MY. Investigation of antioxidant interaction between ascorbic acid and rutin in medicine “Ascorutin”. Current issues in pharmacy and medicine: science and practice [Internet]. 2024Feb.23 [cited 2024Jun.21];17(1):17-20. Available from: http://pharmed.zsmu.edu.ua/article/view/295418