Determination of the isotonicity 1 % solution of sodium 2-((4-amino-5-thiophen-2-ylmethyl)-4H-1,2,4-triazole-3-yl)thio)acetate

Authors

DOI:

https://doi.org/10.14739/2409-2932.2024.1.292556

Keywords:

1,2,4-triazole, active pharmaceutical ingredient, isotonic concentration, osmolality, parenteral dosage form

Abstract

The aim of the work is to justify the composition of a 1 % aqueous solution of sodium 2-((4-amino-5-(thiophen-2-ylmethyl)-4H-1,2,4-triazole-3-yl)thio)acetate for parenteral use.

Materials and methods. The osmolality study of a 1 % solution of sodium 2-((4-amino-5-(thiophen-2-ylmethyl)-4H-1,2,4-triazole-3-yl)thio)acetate was conducted using the cryoscopic method according to the State Pharmacopoeia of Ukraine (2.2.35). The calculations were based on measuring the depression of the crystallization temperature of the 1 % solution of sodium 2-((4-amino-5-(thiophen-2-ylmethyl)-4H-1,2,4-triazole-3-yl)thio)acetate, 0.9 % NaCl solution, and high-purity water (Q3). The measurements of crystallization temperature were performed using a Beckman TL-1 thermometer, which allows for research with high precision up to ± 0.005 °C, making it valuable and most effective for studying isotonic concentration.

Results. Temperature measurements of the crystallization of a 1 % solution under investigation, a 0.9 % NaCl solution, and high-quality purified water were conducted. Calculations of isotonic concentration were performed based on the measurement results.

Conclusions. Based on the obtained experimental data on the depression of the crystallization temperature and the calculations, the amount of sodium chloride necessary for the preparation of an isotonic 1 % aqueous solution of AFI sodium 2-((4-amino-5-thiophen-2-ylmethyl)-4H-1,2,4-triazole-3-yl)thio)acetate for parenteral use was established.

Author Biographies

D. L. Usenko, Zaporizhzhia State Medical and Pharmaceutical University, Ukraine

PhD-student of the Department of Physical and Colloidal Chemistry

A. H. Kaplaushenko, Zaporizhzhia State Medical and Pharmaceutical University, Ukraine

PhD, DSc, Head of the Department of Physical and Colloidal Chemistry

B. O. Varynskyi, Zaporizhzhia State Medical and Pharmaceutical University, Ukraine

PhD, DSc, Associate Professor of the Department of Physical and Colloidal Chemistry

References

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Published

2024-02-23

How to Cite

1.
Usenko DL, Kaplaushenko AH, Varynskyi BO. Determination of the isotonicity 1 % solution of sodium 2-((4-amino-5-thiophen-2-ylmethyl)-4H-1,2,4-triazole-3-yl)thio)acetate. Current issues in pharmacy and medicine: science and practice [Internet]. 2024Feb.23 [cited 2024Jun.21];17(1):31-4. Available from: http://pharmed.zsmu.edu.ua/article/view/292556