Development of a method of quantitative determination of the active substance in Angiolin eye drops by the method of high-performance liquid chromatography
DOI:
https://doi.org/10.14739/2409-2932.2023.3.288356Keywords:
eye drops, 1,2,4-triazole derivatives, analysis, standardization, quantitative determination, high performance liquid chromatographyAbstract
One of the most common eye diseases is a burn injury. Hence, one of the pressing challenges in the field of pharmacy today is the development of new ophthalmic medications, specifically eye drops. Researchers from the Department of Pharmaceutical, Organic, and Bioorganic Chemistry at Zaporizhzhia State Medical and Pharmaceutical University, led by Professor I. A. Mazur, have successfully synthesized a novel compound. This compound is a derivative of 1,2,4-triazole, specifically (S)-2,6-diaminohexanoic acid 3-methyl-1,2,4-triazolyl-5-thioacetate. Notably, this compound demonstrates anti-inflammatory, wound-healing, and reparative activities.
The aim of the work is to develop a method of quantitative determination of the active substance in Angiolin eye drops by the method of high-performance liquid chromatography.
Materials and methods. The research employed a liquid chromatograph equipped with a UV detector. A column Hypersil ODS C-18 measuring 250 by 4.6 millimeters with a particle size of 5 microns was used.
Results. It was determined that the angiolin content in the 1 % eye drops in series 1 falls within the range of 0.985 to 1.010 grams. This indicates that, in terms of the active substance content, the studied series complies with the requirements of the State Pharmacopoeia of Ukraine.
Conclusions. As a result of the conducted research, a method for quantitatively determining the active substance in Angiolin eye drops using high-performance liquid chromatography was developed.
References
Belenichev, I. F., Kucherenko, L. I., Mazur, I. A., Akopyan, R. R., & Portnaya, E. A. (2019). Experimental assessment of the specific activity of a new dosage form of angiolin in cataract modeling. Biological Markers and Guided Therapy, 6(1), 113-120. https://doi.org/10.12988/bmgt.2019.91013
Mazur, I. A., Akopian, R. R., Cherkovska, L. H., Pavliuk, I. V., & Skoryna, D. Yu. (2020). Rozrobka metodyky standartyzatsii ochnykh krapel Anhiolin [Development of standardization methods of Angiolin eye drops]. Current issues in pharmacy and medicine: science and practice, 13(3), 383-387. [in Ukrainian]. https://doi.org/10.14739/2409-2932.2020.3.216214
Kucherenko, L. I., Mazur, I. A., Akopian, R. R., Portna, O. O., & Tkachenko, H. I. (2021). Naukove obgruntuvannia kontsentratsii dopomizhnykh rechovyn dlia vyhotovlennia ochnykh krapel Anhiolin [Scientific substantiation of the concentration of excipients for the manufacture of eye drops Angiolin]. Current issues in pharmacy and medicine: science and practice, 14(1), 52-55. https://doi.org/10.14739/2409-2932.2021.1.226773
State Enterprise Ukrainian Scientific Pharmacopoeial Center of Medicines Quality. (2008, February 1). Derzhavna Farmakopeia Ukrainy. Dopovnennia 2 [The State Pharmacopoeia of Ukraine] (1st ed., Suppl. 1). Kharkiv: State Enterprise Ukrainian Scientific Pharmacopoeial Center of Medicines Quality. [in Ukrainian].
Grizodub, A. I., Evtifeeva, O. A., Proskurina, K. I., & Bezumova, O. V. (2014). Standartizovannaya protsedura validatsii spektrofotometri-cheskikh metodik kolichestvennogo opredeleniya lekarstvennykh sredstv v variante metoda pokazatelya pogloshcheniya. Soobshchenie 1 [A standardized procedure for the validation of spectrophotometric methods for the quantitative determination of drugs in a variant of the absorption index method. Message 1]. Farmakom, (1), 29-39. [in Russian].
State Enterprise Ukrainian Scientific Pharmacopoeial Center of Medicines Quality. (2011). Derzhavna Farmakopeia Ukrainy. Dopovnennia 4 [The State Pharmacopoeia of Ukraine] (1st ed., Suppl. 4). Kharkiv: State Enterprise Ukrainian Scientific Pharmacopoeial Center of Medicines Quality. [in Ukrainian].
Grizodub, A. I. (2016). Standartyzovani protsedury validatsii metodyk kontroliu yakosti likarskykh zasobiv [Standardization of the procedure for validation of methods of quality control of drugs]. State Enterprise "Ukrainian Scientific Pharmacopoeial Center for Drug Quality". [in Ukrainian].
Georgievskiy, G. V. (2013). Analitychne zabezpechennia syntezu, standartyzatsii ta orhanizatsii vyrobnytstva pokhidnykh 1,2,4-tryazolu ta yikh likarskykh form [Analytical software for the synthesis, standardization and organization of manufacturing of derivatives of 1,2,4-triazole and their drug dosage forms]. (Doctoral Dissertation, National University of Pharmacy, Kharkiv). [in Ukrainian]. https://nrat.ukrintei.ua/searchdoc/0513U001273/
Pertsev, I. M. (Ed.). (2010). Dopomizhni rechovyny v tekhnolohii likiv: vplyv na tekhnolohichni, spozhyvchi, ekonomichni kharakterystyky i terapevtychnu efektyvnist. Kharkiv: Zoloti storinky. [in Ukrainian].
Georgievskii, V. P., Georgievskii, G. V., Zinchenko, A. A., Kulikov, A. Yu., Nazarova, E. S., & Kolisnyk, A. V. (2016). Khromatograficheskie metody v analiticheskom obespechenii sozdaniya i kontrolya kachestva lekarstvennykh sredstv v Ukraine. Kharkiv : NTMT. [in Russian].
Downloads
Published
How to Cite
Issue
Section
License
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access)
