Antioxidant, antimicrobial and antifungal activity of the obtained “Cachisept” tablets for resorption in the oral cavity for the treatment and prevention of dental caries
DOI:
https://doi.org/10.14739/2409-2932.2023.3.285425Keywords:
tablets, green tea, dental caries, plant extract, CachiseptAbstract
Dental caries stands as a highly common chronic ailment affecting individuals globally, with susceptibility to this condition spanning their entire lifespan. Consequently, the search for novel medications aimed at treating and preventing dental caries holds significant importance in the field of pharmacy.
The aim of the work was to study the antioxidant, antimicrobial, and antifungal activity of the obtained oral tablets “Сaсhisept” for resorption in the oral cavity for the treatment and prevention of dental caries.
Materials and methods. The obtained tablets “Сaсhisept” with green tea leaf extract were the object of investigation. Antioxidant activity was established by the potentiometric method, and antimicrobial and antifungal activity was assessed by the agar well diffusion method.
Results. The level of antioxidant activity for tablets “Сaсhisept” was 110.00 ± 2.20 mmol-eqv./mdry res, the obtained tablets had a high level of antioxidant activity according to Maslov’s conditional classification. Staphylococcus aureus (25.33 ± 0.50 mm), Baсillus subtilis (25.33 ± 0.50 mm) and Streptococcus mutans (25.33 ± 0.50 mm) were the most sensitive to the obtained “Сaсhisept” tablets, and the least – Proteus vulgaris (20.67 ± 0.50 mm), and it was observed strong growth inhibition in relation to the fungus Candida albicans (20.00 ± 0.50 mm).
Conclusions. The presented study has found that “Сaсhisept” tablets have a high level of antioxidant activity, and also have a high antimicrobial and antifungal effect against all investigated Gram-positive, Gram-negative bacteria, and the Candida albicans fungus. Thus, “Сaсhisept” tablets can be recommended for the treatment and prevention of dental caries.
References
Veiga, N., Aires, D., Douglas, F., Pereira, M., Vaz, A., Rama, L., Silva, M., Miranda, V., Pereira, F., Vidal, B., Plaza, J., & Bexiga, F. (2016). Dental Caries: A Review. Journal of dental and oral health, 2(5), 1-3.
Karpiński, T. M., & Szkaradkiewicz, A. K. (2013). Microbiology of dental caries: M21-M24. Review Journal of Biology and Earth Sciences, 3(1), M21-M24. Retrieved from http://www.journals.tmkarpinski.com/index.php/jbesorhttp://jbes.strefa.pl
Simón-Soro, A., & Mira, A. (2015). Solving the etiology of dental caries. Trends in Microbiology, 23(2), 76-82. https://doi.org/10.1016/j.tim.2014.10.010
Zadorozhna, I. V., & Povoroznyuk, V. V. (2014). Poshyrenist ta intensyvnist kariiesu zubiv u ditei Ukrainy: rezultaty kliniko-epidemiolohichnoho obstezhennia [Prevalence and intensity of dental caries in children of Ukraine: results of a clinical and epidemiological survey]. Problemy osteolohii, 16(4), 55-60. [in Ukrainian].
Bida, O. V., Struk, V. I., & Zabuha, Yu. I. (2013). Analiz stanu stomatolohichnoho zdorovia ta rivnia zuboprotezuvannia naselennia Ukrainy [Analysis of the state of dental health and the level of dental prosthetics of the population in Ukraine]. Zbirnyk naukovykh prats spivrobitnykiv NMAPO im. P. L. Shupyka, 22 (1), 370-377. [in Ukrainian].
Maslov, O. Y., Komisarenko, M. A., Kolisnyk, Y. S., & Kostina, T. A. (2021). Determination of catechins in green tea leaves by HPLC compared to spectrophotometry. Zhurnal orhanichnoi ta farmatsevtychnoi khimii, 19(3), 28-33. https://doi.org/10.24959/ophcj.21.238177
Maslov, O. Yu., Kolisnyk, S. V., Komisarenko, M. A., Akhmedov, E. Y., Poluian, S. M., & Shovkova, Z. V. (2021). Study of flavonoids and phenolic acids in green tea leaves. Current issues in pharmacy and medicine: science and practice, 14(3), 287-291. https://doi.org/10.14739/2409-2932.2021.3.240287
Maslov, O. Yu., Kolisnyk, S. V., Komissarenko, M. A., Komissarenko, A. M., Osolоdchenko, T. P., & Ponomarenko, S. V. (2022). In vitro antioxidant and antibacterial activities of green tea leaves (Camella sinensis l.) liquid extracts. Annals of Mechnikov’s Institute, (2), 64-67. https://doi.org/10.5281/zenodo.6634846
Maslov, O. Yu., Komisarenko, M. A., Golik, M. Yu., Kolisnyk, S. V., Altukhov, A. A., Baiurka, S. V., Karpushina, S. A., Tkachenko, O., & Kolisnyk, I. (2023). Study of total antioxidant capacity of red raspberry (Rubus idaeous L.) shoots. Vitae, 30(1). https://doi.org/10.17533/udea.vitae.v30n1a351486
Volianskyi, Yu. L., Shyrobokov, V. P., Biriukova, S. V. et al. (2004). Vyvchennia spetsyfichnoi aktyvnosti protymikrobnykh likarskykh zasobiv [The study of the specific activity of antimicrobial drugs]. Kyiv.
Volianskyi, Yu. L., Myronenko, L. H., Kalinichenko, S. V., Skliar, N. I., Kolokolova, O. B., Tkach, L. V., & Peretiatko, O. H. (2006). Standartyzatsiia pryhotuvannia mikrobnykh suspenzii [Standardization of the preparation of microbialsuspensions]. Newsletter of innovations in health care No 163-2006. Kharkiv: Ukrmedpatentinform.
Maslov, O. Yu., Komisarenko, M. A., Kolisnyk, S. V., Golik, M. Y., Tsapko, Y. O., & Akhmedov, E. Yu. (2022). Determination of the extraction frequency of green tea leaves by the antioxidant method. Zhurnal orhanichnoi ta farmatsevtychnoi khimii, 20(1), 28-34. https://doi.org/10.24959/ophcj.22.252320
Maslov, O. Yu., Kolisnyk, S. V., Komisarenko, M. A., Kolisnyk, O. V., & Ponomarenko, S. V. (2021). Antioxidant activity of green tea leaves (Camellia sinensis L.) liquid extracts. Pharmacologyonline, 3, 291-298. https://doi.org/10.5281/zenodo.7813115
Maslov, O. Yu., Kolisnyk, S. V., Komisarenko, M. A., Ponomarenko, S. V., Osolodchenko, T. P., Poluian, S. M., Moroz, V. P., Pohosian, O. H., Karpova, S. P., & Bondarenko, N. Yu. (2022). Sposib oderzhannia kofeinu, yakyi proiavliaie antymikrobnu diiu, z lystia zelenoho chaiu [The method of obtaining caffeine, which exhibits an antimicrobial effect, from green tea leaves]. Ukraine Patent UA 151547. Retrieved from: https://sis.nipo.gov.ua/uk/search/detail/1701487/
Maslov, O. Yu., Kolisnyk, S. V., Komisarenko, M. A., Altukhov, O. O., Dynnyk, K. V., & Stepanenko, V. I. (2021). Study and evaluation antioxidant activity of dietary supplements with green tea extract. Current issues in pharmacy and medicine: science and practice, 14(2), 215-219. https://doi.org/10.14739/2409-2932.2021.2.233306
Ahmadi-Motamayel, F., Goodarzi, M., Hendi, S., Kasraei, S., & Moghimbeigi, A. (2013). Total antioxidant capacity of saliva and dental caries. Medicina Oral Patología Oral y Cirugia Bucal, Стаття e553-e556. https://doi.org/10.4317/medoral.18762
Ren, Z., Jeckel, H., Simon-Soro, A., Xiang, Z., Liu, Y., Cavalcanti, I. M., Xiao, J., Tin, N.-N., Hara, A., Drescher, K., & Koo, H. (2022). Interkingdom assemblages in human saliva display group-level surface mobility and disease-promoting emergent functions. Proceedings of the National Academy of Sciences, 119(41), e2209699119. https://doi.org/10.1073/pnas.2209699119
Downloads
Published
How to Cite
Issue
Section
License
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access)
