Spectrophotometric study of tannins in the herb Achillea millefolium L.

Authors

DOI:

https://doi.org/10.14739/2409-2932.2023.2.281344

Keywords:

Achillea millefolium L., tannins, spectrophotometric study, pyrogallol, galic acid

Abstract

Tannins are an important class of secondary metabolites with a wide range of pharmacological effects, due to which they are used in various fields of treatment. In plants of the genus Achillea L., tannins are the dominant class of compounds. This allows the use of plant raw materials and extracts of yarrow for the development of complex herbal and combined formulations of medicines. The search for new sources of natural tannin compounds for pharmaceutical and cosmetic purposes is of significant interest and encourages the improvement of phytochemical research methods of known species of medicinal plants.

The aim of the work was to develop a spectrophotometric method for determining the quantitative composition of tannin compounds in terms of gallic acid in the herb of Achillea millefolium L.

Materials and methods. The study utilized dried air-dried raw material of yarrow for analysis. The quantitative content of polyphenols in the plant material was determined using a modified UV spectrophotometric method, with calculations based on pyrogallol equivalents. The development of the spectrophotometric technique for determining tannins from the herb of Achillea millefolium L., expressed as gallic acid equivalents, considered the effects of extractant concentration and the degree of plant material grinding.

Results. The water extract from the herb of Achillea millefolium L. exhibited a quantitative content of polyphenols, calculated as pyrogallol equivalents, at 2.9781 ± 0.0177 %. It was observed that the particle size of the raw material significantly influenced the yield of active substances. The optimal technological parameters for the developed method involved extracting plant material that was crushed to a size of 0.5–1.0 mm, using 70 % ethanol in a ratio of 1:10, resulting in a yield of 4.08 ± 0.01 %.

Conclusions. Based on the obtained results, it is recommended to utilize the developed method, employing gallic acid equivalents, for the quantification of tannins in alcohol-water extracts of yarrow.

Author Biographies

H. P. Smoilovska, Zaporizhzhia State Medical and Pharmaceutical University, Ukraine

PhD, Associate Professor of the Department of Pharmacy Management and Economics, and Pharmaceutical Technology

O. O. Maliuhina, Zaporizhzhia State Medical and Pharmaceutical University, Ukraine

PhD, Senior Lecturer of the Department of Pharmacy Management and Economics, and Pharmaceutical Technology

O. K. Yerenko, Zaporizhzhia State Medical and Pharmaceutical University, Ukraine

PhD, Senior Lecturer of the Department of Pharmacy Management and Economics, and Pharmaceutical Technology     

T. V. Khortetska, Zaporizhzhia State Medical and Pharmaceutical University, Ukraine

PhD, Associate Professor of the Department of Pharmacy Management and Economics, and Pharmaceutical Technology

References

Agar, O. T., Dikmen, M., Ozturk, N., Yilmaz, M. A., Temel, H., & Turkmenoglu, F. P. (2015). Comparative Studies on Phenolic Composition, Antioxidant, Wound Healing and Cytotoxic Activities of Selected Achillea L. Species Growing in Turkey. Molecules, 20(10), 17976-18000. https://doi.org/10.3390/molecules201017976

Salehi, B., Selamoglu, Z., Sevindik, M., Fahmy, N. M., Al-Sayed, E., El-Shazly, M., Csupor-Löffler, B., Csupor, D., Yazdi, S. E., Sharifi-Rad, J., Arserim-Uçar, D. K., Arserim, E. H., Karazhan, N., Jahani, A., Dey, A., Azadi, H., Vakili, S. A., Sharopov, F., Martins, N., & Büsselberg, D. (2020). Achillea spp.: A comprehensive review on its ethnobotany, phytochemistry, phytopharmacology and industrial applications. Cellular and molecular biology (Noisy-le-Grand, France), 66(4), 78-103. https://doi.org/10.14715/cmb/2020.66.4.13

Ali, S. I., Gopalakrishnan, B., & Venkatesalu, V. (2017). Pharmacognosy, Phytochemistry and Pharmacological Properties of Achillea millefolium L.: A Review. Phytotherapy research : PTR, 31(8), 1140-1161. https://doi.org/10.1002/ptr.5840

Strzępek-Gomółka, M., Gaweł-Bęben, K., & Kukula-Koch, W. (2021). Achillea Species as Sources of Active Phytochemicals for Dermatological and Cosmetic Applications. Oxidative medicine and cellular longevity, 2021, 6643827. https://doi.org/10.1155/2021/6643827

Tadić, V., Arsić, I., Zvezdanović, J., Zugić, A., Cvetković, D., & Pavkov, S. (2017). The estimation of the traditionally used yarrow (Achillea millefolium L. Asteraceae) oil extracts with anti-inflamatory potential in topical application. Journal of ethnopharmacology, 199, 138-148. https://doi.org/10.1016/j.jep.2017.02.002

El-Kalamouni, C., Venskutonis, P. R., Zebib, B., Merah, O., Raynaud, C., & Talou, T. (2017). Antioxidant and Antimicrobial Activities of the Essential Oil of Achillea millefolium L. Grown in France. Medicines, 4(2), 30. https://doi.org/10.3390/medicines4020030

Garzoli, S., Cicaloni, V., Salvini, L., Trespidi, G., Iriti, M., & Vitalini, S. (2023). SPME-GC-MS Analysis of the Volatile Profile of Three Fresh Yarrow (Achillea millefolium L.) Morphotypes from Different Regions of Northern Italy. Separations, 10(1). https://doi.org/10.3390/separations10010051

Duyun, I. F., Mazulin, O. V., Мazulin, G. V., & Oproshanska, Т. V. (2020). Khimichnyi sklad polifenolnykh spoluk u travi dereviiu podovoho (Achillea micranthoides Klok.) [The chemical composition of polyphenolic compounds Achillea micranthoides Klok. herbs]. Farmatsevtychnyi zhurnal, 75(1), 80-87. [in Ukrainian]. https://doi.org/10.32352/0367-3057.1.20.09

Serdar, G., Sökmen, M., Demir, E., Sökmen, A., & Bektaş, E. (2016). Extraction of antioxidative principles of Achillea biserrata M. Bieb. and chromatographic analyses. International Journal of Secondary Metabolite, 2(2), 3-15. https://doi.org/10.21448/ijsm.240706

Angourani, H. R., Zarei, A., Moghadam, M. M., Ramazani, A., & Mastinu, A. (2023). Investigation on the Essential Oils of the Achillea Species: From Chemical Analysis to the In Silico Uptake against SARS-CoV-2 Main Protease. Life, 13(2), 378. https://doi.org/10.3390/life13020378

Marinas, I. C., Oprea, E., Gaboreanu, D. M., Gradisteanu Pircalabioru, G., Buleandra, M., Nagoda, E., Badea, I. A., & Chifiriuc, M. C. (2023). Chemical and Biological Studies of Achillea setacea Herba Essential Oil-First Report on Some Antimicrobial and Antipathogenic Features. Antibiotics, 12(2), 371. https://doi.org/10.3390/antibiotics12020371

Ladan Moghadam, A. R. (2017). New compound from the aerial parts of Achillea millefolium. International Journal of Food Properties, 20(9), 2041-2051. https://doi.org/10.1080/10942912.2016.1230747

Ahmadi-Dastgerdi, A., Ezzatpanah, H., Asgary, S., Dokhani, S., & Rahimi, E. (2017). Phytochemical, Antioxidant and Antimicrobial Activity of the Essential Oil from Flowers and Leaves of Achillea millefolium subsp. millefolium. Journal of Essential Oil-Bearing Plants, 20(2), 395-409. https://doi.org/10.1080/0972060X.2017.1280419

Marchyshyn, C. M., Budniak, L. I., & Ivasiuk, I. M. (2020). Doslidzhennia dubylnykh rechovyn u travi ta bulbakh smykavtsia yistivnoho (chufy) (Cyperus esculentus L.) metodom VERKh [Investigation of tannins in herb and tubers of the yellow nutgrass (Cyperus esculentus L.) (chufa) by HPLC method]. Current issues in pharmacy and medicine: science and practice, 13(2), 225-229. [in Ukrainian]. https://doi.org/10.14739/2409-2932.2020.2.207119

Jing, W., Xiaolan, C., Yu, C., Feng, Q., & Haifeng, Y. (2022). Pharmacological effects and mechanisms of tannic acid. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 154, 113561. https://doi.org/10.1016/j.biopha.2022.113561

Fraga-Corral, M., Otero, P., Cassani, L., Echave, J., Garcia-Oliveira, P., Carpena, M., Chamorro, F., Lourenço-Lopes, C., Prieto, M. A., & Simal-Gandara, J. (2021). Traditional Applications of Tannin Rich Extracts Supported by Scientific Data: Chemical Composition, Bioavailability and Bioaccessibility. Foods, 10(2), 251. https://doi.org/10.3390/foods10020251

State Enterprise Ukrainian Scientific Pharmacopoeial Center of Medicines Quality. (2014). Derzhavna Farmakopeia Ukrainy [The State Pharmacopoeia of Ukraine (Vol. 3, 2nd ed.)]. Kharkiv: State Enterprise Ukrainian Scientific Pharmacopoeial Center of Medicines Quality. [in Ukrainian].

State Enterprise Ukrainian Scientific Pharmacopoeial Center of Medicines Quality. (2015). Derzhavna Farmakopeia Ukrainy [The State Pharmacopoeia of Ukraine (Vol. 1, 2nd ed.)]. Kharkiv: State Enterprise Ukrainian Scientific Pharmacopoeial Center of Medicines Quality. [in Ukrainian].

Nayeem, N., & SMB, A. (2016). Gallic Acid: A Promising Lead Molecule for Drug Development. Journal of Applied Pharmacy, 08(02). https://doi.org/10.4172/1920-4159.1000213

Published

2023-07-03

How to Cite

1.
Smoilovska HP, Maliuhina OO, Yerenko OK, Khortetska TV. Spectrophotometric study of tannins in the herb Achillea millefolium L. Current issues in pharmacy and medicine: science and practice [Internet]. 2023Jul.3 [cited 2024Apr.14];16(2):130-4. Available from: http://pharmed.zsmu.edu.ua/article/view/281344

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Section

Original research