Development of a spectrophotometric technique for the quantitative determination of ademol




spectrophotometry, analysis, ademol, bromocresol green, State Pharmacopoeia of Ukraine, validation


According to statistics, craniocerebral injuries are often the cause of disability among the population. The choice of medicines for traumatic brain injuries is one of the most difficult problems in the complex treatment of such patients. Ademol (1-adamantylethyloxy-3-morpholino-2-propanol hydrochloride) is a promising medicinal substance that has a huge positive therapeutic effect of a protective act on the damaged brain and can be presented in medicinal preparations in different dosages. Therefore, the urgent task is the development of highly accurate, reliable, affordable, and fast methods of quantitative determination of ademol.

The aim of the work is to study the optimal conditions for the “ademol – bromocresol green” reaction and to develop an express, sensitive and easy-to-implement method for the quantitative determination of ademol.

Materials and methods. Bromocresol green (BCG) grade “chemically pure” and acetone grade “pure for analysis” were used as reagents and solvents. Analytical equipment: spectrophotometer Specord 200, electronic scales AVT-120-5DM, ultrasonic bath Elmasonic E 60H, class A measuring vessels.

Results. A new method of quantitative determination of ademol by the spectrophotometric method was developed. The optimal conditions of the flow between the substance to be determined and the reagent have been studied, the concentration limits have been established, in which subordination to the basic law of light absorption is observed. A forecast of the complete uncertainty of the results of the specified method of quantitative determination was made to assess the correctness of the reproduction of the developed method in other laboratories. The proposed method is relevant according to the requirements of the State Pharmacopoeia of Ukraine.

Conclusions. According to the experimental data, the method of quantitative determination of ademol can be correctly reproduced and suitable for use.

Author Biographies

S. I. Semenenko, National Pirogov Memorial Medical University, Vinnytsya, Ukraine

MD, PhD, Associate Professor, Head of the Department of Clinical Pharmacy and Clinical Pharmacology

K. P. Miedviedieva, Zaporizhzhia State Medical University, Ukraine

PhD, Associate Professor of the Department of Analytical Chemistry

S. O. Vasiuk, Zaporizhzhia State Medical University, Ukraine

PhD, DSc, Professor, Head of the Department of Analytical Chemistry

B. S. Burlaka, Zaporizhzhia State Medical University, Ukraine

PhD, DSc, Associate Professor of the Department of Medicines Technology


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How to Cite

Semenenko SI, Miedviedieva KP, Vasiuk SO, Burlaka BS. Development of a spectrophotometric technique for the quantitative determination of ademol. CIPM [Internet]. 2023Mar.10 [cited 2023Dec.10];16(1):28-32. Available from:



Original research