Study the polyphenolic compounds composition of Thymus vulgaris L. herb

Authors

DOI:

https://doi.org/10.14739/2409-2932.2023.1.265524

Keywords:

Thymus vulgaris L., TLC, HPLC, medicinal herb, anti-inflammatory activity, antimicrobial activity

Abstract

The Thymus L. genus is extremely widespread on the territory of Ukraine and includes up to 50 main species. Extracts from the official species of the Thymus L. genus are part of effective herbal preparations with pronounced anti-inflammatory, antimicrobial and antioxidant activities. A promising direction of modern phytochemical research is the determination of the accumulation of biologically active polyphenolic compounds in the Thymus vulgaris L. herb, which is widely cultivated in Ukraine and other countries.

The aim of the work is to determine the accumulation of polyphenolic compounds in Thymus vulgaris L. herb at the end of flowering using TLC and HPLC methods.

Materials and methods. For experimental studies, alcohol extracts from Thymus vulgaris L. herb (1:100) were used. The plants were cultivated in Volodymyrivka, Zaporizhzhia region during the period of maximum polyphenolic compounds accumulation (June – August 2022). The substances’ component composition was studied by TLC method Biostep CD 60 densitometer (Germany) and HPLC Agilent 1260 Infinity HPLC System Open LABCDS Software (Japan).

Results. TLC and HPLC methods revealed up to 29 polyphenolic compounds. Experimentally determined flavonoids and hydroxycinnamic acids are known in phytotherapy for their pronounced anti-inflammatory, antimicrobial, and antioxidant activities.

Conclusions. Considering the results obtained by TLC and HPLC methods, it can be concluded that Thymus vulgaris L. is a promising species for further phytochemical research.

Author Biographies

O. V. Mazulin, Zaporizhzhia State Medical University, Ukraine

PhD, DSc, Professor of the Department of Clinical Pharmacy, Pharmacotherapy, Pharmacognosy and Pharmaceutical Chemistry

L. A. Fukleva, Zaporizhzhia State Medical University, Ukraine

PhD, Senior Lecturer of the Department of Pharmacy Management and Economics, and Pharmaceutical Technology

G. V. Mazulin, Zaporizhzhia State Medical University, Ukraine

PhD, Senior Lecturer of the Department of Pharmacognosy, Pharmacology and Botany

References

Nachychko, V. O, & Honcharenko, V. I. (2016). Hybrydy rodu Thymus L. (Lamiaceae) u flori zakidnykh rehioniv Ukrainy: taksonomichnyi sklad i poshyrennia. [Hybrids of Thymus L. (Lamiaceae) genus in flora of the western regions of Ukraine: taxonomic composition and distribution]. Visnik Lvivskoho Universitetu. Seriia biolohichna, 10(1), 163-186. [in Ukrainian]. https://dx.doi.org/10.30970/sbi.1001.442

Nachychko, V. O, & Honcharenko, V. I. (2017). Botaniko-heohrafichna kharakterystyka vydiv rodu Thymus L. (Lamiaceae) u flori zakhidnykh rehioniv Ukrainy [Botanical-geographical characterization of Thymus L. (Lamiaceae) species in the flora of western regions of Ukraine]. Visnik Lvivskoho Universitetu. Seriia biolohichna, 75, 35-47. [in Ukrainian]. https://dx.doi.org/10.30970/vlubs.2017.75.04

Afonso, A. F., Pereira, O. R., Válega, M., Silva, A. M. S., & Cardoso, S. M. (2018). Metabolites and Biological Activities of Thymus zygis, Thymus pulegioides, and Thymus fragrantissimus Grown under Organic Cultivation. Molecules, 23(7), 1514. https://doi.org/10.3390/molecules23071514

Medicinal and Functional Values of Thyme (Thymus vulgaris L.) Herb. (2017). Journal of Applied Biology & Biotechnology, 5(2), 017-022. https://doi.org/10.7324/jabb.2017.50203

State Enterprise Ukrainian Scientific Pharmacopoeial Center of Medicines Quality. (2009). Derzhavna Farmakopeia Ukrainy. Dopovnennia 3 [The State Pharmacopoeia of Ukraine] (1st ed., Suppl 3). Kharkiv: Naukovo-ekspertnyi farmakopeinyi tsentr. [in Ukrainian].

Tohidi, B., Rahimmalek, M., & Arzani, A. (2017). Essential oil composition, total phenolic, flavonoid contents, and antioxidant activity of Thymus species collected from different regions of Iran. Food chemistry, 220, 153-161. https://doi.org/10.1016/j.foodchem.2016.09.203

Zarivna, N. О. (2021). Rozrobka metodyky identyfikatsii monosakcharydiv u ridkomu ekstrakti chebretsiu povzuchoho [Development of monosaccharides identification methods in Thyme creeping extracts]. Medychna ta klinichna khimiia, 23(1), 80-83. [in Ukrainian]. https://dx.doi.org/10.11603/mcch.2410-681x.2021.i1.12112

Zarivna, N. O., & Logoyda, L. S. (2020). Rozrobka metodyky identyfikatsii flavonoydiv ta hidroksykorychnykh kyslot v ekstraktakh chebretsiu povzuchoho [Development of flavonoid and hydroxycinnamic acid identification methods in Thyme creeping extracts]. Medychna ta klinichna khimiia, 22(1), 107-111. [in Ukrainian]. https://doi.org/10.11603/mcch.2410-681X.2020.v.i1.11061

Toncer, O., Karaman, S., Diraz, E., Sogut, T., & Kizil, S. (2017). Essential oil composition of Thymus × citriodorus (Pers.) Schreb. at different harvest stages. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 45(1), 185-189. https://doi.org/10.15835/nbha45110672

Vetvicka, V., & Vetvickova, J. (2016). Essential Oils from Thyme (Thymus vulgaris): Chemical Composition and Biological Effects in Mouse Model. Journal of medicinal food, 19(12), 1180-1187. https://doi.org/10.1089/jmf.2016.0029

Svydenko, L. V., & Hlushchenko, L. A. (2016). Komponentnyi sklad efirnoi olii u formakh vydiv chebretsiu povzuchoho (Thymus serpillum L.) i bloshynoho (Thymus pulegioides L.) v umovakh Khersonskoi oblasti [Component composition of essential oil in the forms of thymus serpyllum (Thymus serpillum L.) and thyme flea (Thymus pulegioides L.) species in conditions of Kherson region]. Ahroekolohichnyi zhurnal, (2), 129-134. [in Ukrainian]. https://doi.org/10.33730/2077-4893.2.2016.249116

Oubihi, A., Ouryemchi, I., Nounah, I., Tarfaoui, K., Harhar, H., Ouhssine, M., & Guessous, Z. (2020). Chemical composition, antibacterial and antifungal activities of Thymus leptobotrys Murb essential oil. Advances in Traditional Medicine, 20(4), 673-679. https://doi.org/10.1007/s13596-020-00488-w

Aouam, I., Elatki, Y., Taroq, A., Elkamari, F., Elyoussi, B., & Abdellaoui, A. (2018). Chemical Composition and Antibacterial Activity of the Essential oil of Thymus citriodorus L. Growing Wild in Morocco: Preventive Approach against Nosocomial Infections. International Journal of Pharmaceutical Sciences Review and Research, 51(1), 72-75.

Kryvtsova, M. V., Salamon, I., Koscova, J., Bucko, D., & Spivak, M. (2019). Antimicrobial, antibiofilm and biochemichal properties of Thymus vulgaris essential oil against clinical isolates of opportunistic infections. Biosystems Diversity, 27(3), 270-275. https://doi.org/10.15421/011936

Beicu, R., Alexa, E., Obiștioiu, D., Cocan, I., Imbrea, F., Pop, G., Circioban, D., Moisa, C., Lupitu, A., Copolovici, L., Copolovici, D. M., & Imbrea, I. M. (2021). Antimicrobial Potential and Phytochemical Profile of Wild and Cultivated Populations of Thyme (Thymus sp.) Growing in Western Romania. Plants (Basel, Switzerland), 10(9), 1833. https://doi.org/10.3390/plants10091833

Wisam, S. U., Nahla, T. K., & Tariq, N. M. (2017). Antioxidant activities of thyme extracts. Pakistan Journal of Nutrition, 17(1), 46-50. https://doi.org/10.3923/pjn.2018.46.50

Komaki, A., Hoseini, F., Shahidi, S., & Baharlouei, N. (2015). Study of the effect of extract of Thymus vulgaris on anxiety in male rats. Journal of traditional and complementary medicine, 6(3), 257-261. https://doi.org/10.1016/j.jtcme.2015.01.001

Mazandarani, M., & Ghafourian, M. (2017). Influence of extraction methods on total phenolic, flavonoids and antioxidant activity of Thymus kotchyanus L. extract in Semnan Province, Iran. Trends in Phytochemical Research, 1(1), 33-38.

Niculae, M., Hanganu, D., Oniga, I., Benedec, D., Ielciu, I., Giupana, R., Sandru, C. D., Ciocârlan, N., & Spinu, M. (2019). Phytochemical Profile and Antimicrobial Potential of Extracts Obtained from Thymus marschallianus Willd. Molecules, 24(17), 3101. https://doi.org/10.3390/molecules24173101

State Enterprise Ukrainian Scientific Pharmacopoeial Center of Medicines Quality. (2008, February 1). Derzhavna Farmakopeia Ukrainy. Dopovnennia 2 [The State Pharmacopoeia of Ukraine] (1st ed., Suppl 2). Kharkiv: Naukovo-ekspertnyi farmakopeinyi tsentr. [in Ukrainian].

Downloads

Published

2023-03-10

How to Cite

1.
Mazulin OV, Fukleva LA, Mazulin GV. Study the polyphenolic compounds composition of Thymus vulgaris L. herb. Current issues in pharmacy and medicine: science and practice [Internet]. 2023Mar.10 [cited 2024Nov.26];16(1):23-7. Available from: http://pharmed.zsmu.edu.ua/article/view/265524

Issue

Vol. 16 No. 1 (2023)

Section

Original research

License

Authors who publish with this journal agree to the following terms:

    1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.     Лицензия Creative Commons
    2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
    3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access)