Microscopic, submicroscopic characterization of pro- and anti-inflammatory cell phenotypes of the lungs in conditions of experimental allergic inflammation





electron microscopy, exocrine bronchiolar cell, mast cell, respiratory endocrine cell, goblet cells, guinea pigs


The aim is to study the microscopic and submicroscopic characteristics of pro- and anti-inflammatory cell phenotypes of the lungs under conditions of experimental allergic inflammation.

Material and methods. We used histological and electron microscopic methods to study the lungs of 48 male guinea pigs in experimental ovalbumin-induced allergic inflammation, simulated by subcutaneous sensitization and subsequent intranasal inhalation with ovalbumin. Submicroscopic changes of respiratory endocrine cells, goblet cells, exocrine bronchiolar cells, mast cells, macrophages, eosinophils, endothelial cells of guinea pigs lungs were determined.

Results. The most significant reactive submicroscopic changes were established on the 23rd and 30th days of observation in the form of an increase in the functional activity of exocrine bronchiolar and goblet cells, as evidenced by the presence of a light nucleus with a predominance of euchromatin, nucleoplasm of low electron-optical density, nucleoli, developed granular endoplasmic reticulum and an increase in the number of goblet cells secretory mucous granules by electron microscopic examination. The revealed ultramicroscopic features of respiratory endocrine cells (an increase in a number of “empty” core dense vesicles), eosinophilic granulocytes (piecemeal degranulation), an increase in the number of mast cells granules, numerous pseudopodia in macrophages are the confirmation of the active participation of these cell phenotypes in the initiation of inflammation during the early period of the allergic inflammatory process in lungs.

Conclusions. A significant reaction of the innate nonspecific and adaptive immunity occurs in airways during the experimental ovalbumin-induced allergic inflammation, consisting primarily of the functional activation of eosinophilic granulocytes, mast cells, and macrophages, as well as an increase in the secretory activity of exocrine bronchiolar cells and goblet cells, which is confirmed by the changes investigated by electron microscopic examination and are accompanied by reactive changes in the vessels of microcirculatory bed.

Author Biography

S. S. Popko, Zaporizhzhia State Medical University, Ukraine

PhD, Associate Professor of the Department of Histology, Cytology and Embryology


Carlini, F., Picard, C., Garulli, C., Piquemal, D., Roubertoux, P., Chiaroni, J., Chanez, P., Gras, D., & Di Cristofaro, J. (2017). Bronchial Epithelial Cells from Asthmatic Patients Display Less Functional HLA-G Isoform Expression. Frontiers in immunology, 8, 6. https://doi.org/10.3389/fimmu.2017.00006

Kiboneka, A., & Kibuule, D. (2019). The Immunology of Asthma and Allergic Rhinitis. In Rhinosinusitis. IntechOpen. https://doi.org/10.5772/intechopen.86964

Wang, W., Li, Y., Lv, Z., Chen, Y., Li, Y., Huang, K., Corrigan, C. J., & Ying, S. (2018). Bronchial Allergen Challenge of Patients with Atopic Asthma Triggers an Alarmin (IL-33, TSLP, and IL-25) Response in the Airways Epithelium and Submucosa. Journal of immunology, 201(8), 2221-2231. https://doi.org/10.4049/jimmunol.1800709

Barrios, J., Patel, K. R., Aven, L., Achey, R., Minns, M. S., Lee, Y., Trinkaus-Randall, V. E., & Ai, X. (2017). Early life allergen-induced mucus overproduction requires augmented neural stimulation of pulmonary neuroendocrine cell secretion. FASEB journal, 31(9), 4117-4128. https://doi.org/10.1096/fj.201700115R

McBrien, C. N., & Menzies-Gow, A. (2017). The Biology of Eosinophils and Their Role in Asthma. Frontiers in medicine, 4, 93. https://doi.org/10.3389/fmed.2017.00093

Elieh Ali Komi, D., & Bjermer, L. (2019). Mast Cell-Mediated Orchestration of the Immune Responses in Human Allergic Asthma: Current Insights. Clinical Reviews in Allergy and Immunology, 56, 234-247. https://doi.org/10.1007/s12016-018-8720-1

Méndez-Enríquez, E., & Hallgren, J. (2019). Mast Cells and Their Progenitors in Allergic Asthma. Frontiers in immunology, 10, 821. https://doi.org/10.3389/fimmu.2019.00821

Rokicki, W., Rokicki, M., Wojtacha, J., & Dżeljijli, A. (2016). The role and importance of club cells (Clara cells) in the pathogenesis of some respiratory diseases. Kardiochirurgia i torakochirurgia polska = Polish journal of cardio-thoracic surgery, 13(1), 26-30. https://doi.org/10.5114/kitp.2016.58961

Cai, Z., Liu, J., Bian, H., & Cai, J. (2019). Albiflorin alleviates ovalbumin (OVA)-induced pulmonary inflammation in asthmatic mice. American journal of translational research, 11(12), 7300-7309.

Dey, P. (2018). Basic and advanced laboratory techniques in histopathology and cytology. Basic and Advanced Laboratory Techniques in Histopathology and Cytology (pp. 1-275). Springer Singapore. https://doi.org/10.1007/9789811082528

Banno, A., Reddy, A. T., Lakshmi, S. P., & Reddy, R. C. (2020). Bidirectional interaction of airway epithelial remodeling and inflammation in asthma. Clinical science, 134(9), 1063-1079. https://doi.org/10.1042/CS20191309

Popko, S. S. (2021). Morphological rearrangement of the metabolic link of the microcirculatory bed of guinea pigs lungs after sensitization with ovalbumin. Current issues in pharmacy and medicine: science and practice, 14(1), 79-83. https://doi.org/10.14739/2409-2932.2021.1.226851

Popko, S., Yevtushenko, V., Kaplaushenko, A., & Tertishniy, S. (2022). The resistive region of pulmonary microvessels in ovalbumin-sensitised guinea pigs: a quantitative and qualitative histological study. Biologija, 68(1), 43-51. https://doi.org/10.6001/biologija.v68i1.4703

Barrios, J., Kho, A. T., Aven, L., Mitchel, J. A., Park, J. A., Randell, S. H., Miller, L. A., Tantisira, K. G., & Ai, X. (2019). Pulmonary Neuroendocrine Cells Secrete γ-Aminobutyric Acid to Induce Goblet Cell Hyperplasia in Primate Models. American journal of respiratory cell and molecular biology, 60(6), 687-694. https://doi.org/10.1165/rcmb.2018-0179OC

Garg, A., Sui, P., Verheyden, J. M., Young, L. R., & Sun, X. (2019). Consider the lung as a sensory organ: A tip from pulmonary neuroendocrine cells. Current topics in developmental biology, 132, 67-89. https://doi.org/10.1016/bs.ctdb.2018.12.002

Sui, P., Wiesner, D. L., Xu, J., Zhang, Y., Lee, J., Van Dyken, S., Lashua, A., Yu, C., Klein, B. S., Locksley, R. M., Deutsch, G., & Sun, X. (2018). Pulmonary neuroendocrine cells amplify allergic asthma responses. Science, 360(6393), eaan8546. https://doi.org/10.1126/science.aan8546

Katoh S. (2022). Critical Involvement of CD44 in T Helper Type 2 Cell-Mediated Eosinophilic Airway Inflammation in a Mouse Model of Acute Asthma. Frontiers in immunology, 12, 811600. https://doi.org/10.3389/fimmu.2021.811600

Popko, S. S., Yevtushenko, V. M., & Zidrashko, H. A. (2022). Characteristics of CD56-positive cells in guinea pig lung in the dynamics of experimental allergic inflammation. Zaporozhye Medical Journal, 24(1), 79-83. https://doi.org/10.14739/2310-1210.2022.1.235880




How to Cite

Popko SS. Microscopic, submicroscopic characterization of pro- and anti-inflammatory cell phenotypes of the lungs in conditions of experimental allergic inflammation. CIPM [Internet]. 2022Nov.15 [cited 2023Dec.8];15(3):288-94. Available from: http://pharmed.zsmu.edu.ua/article/view/264993



Original research