The development of quantative and qualitive analysis methods of suppositories with Maclura Pomifera extract

Authors

  • V. A. Korotkov
  • A. S. Kukhtenko
  • N. U. Bevz
  • V. A. Grudko
  • E. V. Gladuh

DOI:

https://doi.org/10.14739/2409-2932.2014.2.26131

Keywords:

Standards, Phytosterols, Triterpenes, Isoflavones, Suppositories

Abstract

Chronic prostatitis and BPH are still very common diseases. In recent years, herbal preparations are widely used in the treatment of prostate diseases gland. The effectiveness of herbal medicinal products derived from MacluraPomifera is associated with their content of phytosterols and terpenes. Derived oil extract of MacluraPomiferafruit Orange (Maclurapomifera, Moraceae) is a rich source of terpenes and phytosterols. Previous studies indicated, that the content of such substances as lupeol and β-sitosterol, which is known its prostatoprotectors properties as well as the presence of isoflavones possessing anti-inflammatory and antioxidant properties.

Aim of the work

The aim of this work is a developing of methods that allow assaying qualitative and quantitative assessment of the suppositories with MacluraPomifera extract.

Materials and methods

Theobjects of this study are the suppositories with oil extract of MacluraPomifera. For the suppositories ingredients’identification thin layer chromatography has been used. Quantitative determination of active compounds has been carried out by spectrophotometry in ultraviolet and visible spectrum. The spectrophotometers of Thermo Scientific Evolution S60 (USA) and Apel PD303S (Japan) have been used. Determination of phytosterols and triterpenes amount has been performed in the equivalent of a reliable sample of lupeol ('Santa Cruz Biotechnology', USA; CAS: 545-47-1). Determination of isoflavones amount has been carried out in the equivalent of a reliable sample osayin ('BioBioPhaCo., Ltd.',China; CAS: 482-53-1).

Results and discussion

For identification of phytosterols and isoflavones in the suppositories composition a method of identifying their joint presence by TLC has been developed. Inalcoholic extraction from suppository mass two purple spots with Rf 0,8 and 0,57 are observed at the level of spots solution (lupeol and β-sitosterol) and two yellow spots with Rf 0,45 and 0,21 are observed at the level of spots solution comparison (osain and pomiferin).

For the development of the quantitative determination method fortriterpenes and phytosterols amount in the suppositories we used their ability to move in the non-polar solvents from unsaponifiable fraction, and further to give colored complexes by interreaction with concentrated sulfuric acid. The absorbency of the derived colored solution has been determined at spectrophotometer at a wavelength of 309 nm.

The method of differential spectrophotometry based on the selective interaction of aluminum chloride solution with the compounds of the flavonoid structure has been used for the quantitative determination of the isoflavones amount. Absorbance was measured in the visible spectrum at 419 nm.

Thus, we have developed the methods that allow making qualitative and quantitative assessment of suppositories with MacluraPomifera extract. Relative mistake did not exceed ± 3,13% for the amount of phytosterols and triterpenes, and ± 2,24% for the amount of isoflavones.

 

References

Pereverzev, A. S., Sergienko, N. F., & Ilyuhin Yu. A. (2005) Zabolevaniya predstatel'noj zhelezy [Diseases of the prostate]. Kharkiv: Naukova dumka. [in Ukrainian].

Al-Shukri, S. H., & Solihov, D. N. (2009) Sovremennye metody lecheniya khronicheskogo prostatita (obzor literatury) [Modern methods of treatment of chronic prostatitis]. Nephrologiya, 13(2), 86–91. [in Russian].

Korotkov, V. A. (2013) Sravnitel'nyj analiz rynka prostatoprotektorov Kazahstana, Rossii i Ukrainy [Comparative Market Analysis prostatoprotektorov Kazakhstan, Russia and Ukraine]. Vestnik JuKGFA, 3(64), 6–10. [in Kazakhstan].

Miroschnikov, V. (2005) Lekarstvennye rasteniya i preparaty rastitel'nogo proiskhozhdeniya v urologii [Medicinal plants and herbal drugs in urology]. Moscow: MEDpress-Inform. [in Russian].

Korotkov, V. A., Kukhtenko, O. S., Gladuh, Eu. V. (2013) Vybir optymalnoi tekhnolohii otrymannia maslianoho ekstraktu plodiv makliury [Choice Optimal technology for the oil extract of maklyury]. Farmatsevtychni zhurnal, 6, 24–29. [in Ukrainian].

Saloua, F., Eddine, N. I., & Hedi, Z. (2009) Chemical composition and profile characteristics of Osage orange (Maclurapomifera). Industrial crops and products, 29(1), 1–8. doi: 10.1016/j.indcrop.2008.04.013.

Gallo, M. B. C., & Sarachine, M. J. (2009) Biological activities of lupeol. Int. J. Biomed. Pharm. Sci., 3(1), 44–66.

Tsao, R., Yang, R., & Young, J. C. (2003) Antioxidant isoflavones in osage orange, Maclurapomifera (Raf.) Schneid. Journal of agricultural and food chemistry, 51(22), 6445–6451. doi: 10.1021/jf0342369.

(2008) State Pharmacopoeia of the Republic of Kazakhstan. Astana. [in Kazakhstan].

(2001) Derzhavna farmakopeia Ukrainy [State Pharmacopoeia of Ukraine]. Kharkiv: RIREH. [in Ukrainian].

How to Cite

1.
Korotkov VA, Kukhtenko AS, Bevz NU, Grudko VA, Gladuh EV. The development of quantative and qualitive analysis methods of suppositories with Maclura Pomifera extract. Current issues in pharmacy and medicine: science and practice [Internet]. 2014Jul.8 [cited 2024Oct.10];(2). Available from: http://pharmed.zsmu.edu.ua/article/view/26131

Issue

Section

Analysis and standardization of biologycally active substances and dosage forms