Determination of biologically active substances extraction parameters from cranberry leaves

Authors

DOI:

https://doi.org/10.14739/2409-2932.2022.2.258524

Keywords:

cranberry, leaves, extraction, phenolic compounds

Abstract

The problem of type 2 diabetes due to its prevalence, severity, and complications is one of the most pressing in the world. Plants of the genus Vaccinium are promising sources for the creation of hypoglycemic and hypolipidemic medicines. Studies have shown that the extract from cranberry leaves, obtained with 50 % ethanol solution has prospects to be used for correcting insulin resistance. Therefore, for creating new medicines based on cranberry leaves, it is necessary to determine the optimal parameters of biologically active substances (BAS) extraction from this raw material.

The aim of the research is to determine the parameters of BAS extraction from cranberry leaves (Oxycoccus macrocarpus (Ait.) Pursh) for creating a new dry extract with hypoglycemic activity.

Materials and methods. The object of the study was cranberry leaves. Extracts from the leaves were obtained with 50 % ethanol solution in a ratio of 1:10; 1:15; 1:20; 1:25; 1:30 and 1:35 by maceration at room temperature for 8 hours. The extraction was repeated four times with new portions of the extractant, collecting each extract separately. Identification of phenolic compounds was performed by thin-layer chromatography. An assay of phenolic compounds was carried out by spectrophotometric methods. The mass transfer coefficient of each stage (miBAS/Viextragent) was calculated for each indicator and the dependence of these factors on the extraction multiplicity to determine the rational extraction was derived using the software Statistics.

Results. Gallic and chlorogenic acids, rutin, quercetin, and hyperoside were detected on TLC of cranberry leaves extracts in comparison with standard samples of substances. The content of the sum of hydroxycinnamic acid derivatives in terms of chlorogenic acid, the number of flavonoids in terms of rutin, and the content of the sum of phenolic compounds in terms of gallic acid were determined. It was established that the exhaustive extraction of BAS with 50 % ethanol solution from the cranberry leaves provides a ratio of 1 to 100–110. It was rational to carry out BAS extraction from cranberry leaves in a ratio of 1:10 twice, this would ensure the maximum yield of BAS at the minimum cost of the extractant.

Conclusions. It was found that was rational to extract BAS with 50 % ethanol solution from cranberry leaves in a ratio of 1:10 twice using mathematical and statistical methods. It would be used in the future in the development of extract technology.

Author Biographies

I. K. Vlasova, National University of Pharmacy, Kharkiv, Ukraine

PhD student, Department of Pharmacognosy

O. M. Koshovyi, National University of Pharmacy

DSc, Professor of the Department of Pharmacognosy

O. S. Kukhtenko, National University of Pharmacy, Kharkiv, Ukraine

DSc, Professor, Head of the Department of Pharmaceutical Technology

M. A. Komisarenko , National University of Pharmacy, Kharkiv, Ukraine

Assistant of the Department of Pharmacognosy

Т. V. Ilina, National University of Pharmacy, Kharkiv, Ukraine

DSc, Professor of the Department of Pharmacognosy

A. M. Kovalyova, National University of Pharmacy, Kharkiv, Ukraine

DSc, Professor of the Department of Pharmacognosy

References

Harding, J. L., Pavkov, M. E., Magliano, D. J., Shaw, J. E., & Gregg, E. W. (2019). Global trends in diabetes complications: a review of current evidence. Diabetologia, 62(1), 3-16. https://doi.org/10.1007/s00125-018-4711-2

Westman, E. C. (2021). Type 2 Diabetes Mellitus: A Pathophysiologic Perspective. Frontiers in nutrition, 8, 707371. https://doi.org/10.3389/fnut.2021.707371

Sharma, V. K., & Singh, T. G. (2020). Chronic Stress and Diabetes Mellitus: Interwoven Pathologies. Current diabetes reviews, 16(6), 546-556. https://doi.org/10.2174/1573399815666191111152248

Yaribeygi, H., Farrokhi, F. R., Butler, A. E., & Sahebkar, A. (2019). Insulin resistance: Review of the underlying molecular mechanisms. Journal of cellular physiology, 234(6), 8152-8161. https://doi.org/10.1002/jcp.27603

Padhi, S., Nayak, A. K., & Behera, A. (2020). Type II diabetes mellitus: a review on recent drug based therapeutics. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 131, 110708. https://doi.org/10.1016/j.biopha.2020.110708

Ayepola, O. R., Brooks, N. L., & Oguntibeju, O. O. (2014). Oxidative Stress and Diabetic Complications: The Role of Antioxidant Vitamins and Flavonoids. In Antioxidant-Antidiabetic Agents and Human Health. IntechOpen. https://doi.org/10.5772/57282

Koshovyi, O. M., Zagayko, A. L., Kolychev, I. O., Akhmedov, E. Yu., & Komissarenko, A. N. (2016). Phytochemical study of the dry extract from bilberry leaves. Azerbaijan Pharmaceutical and Pharmacotherapy Journal, 16(1), 18-23.

Zagayko, A. L., Kolisnyk, T. Y., Chumak, O. I., Ruban, O. A., & Koshovyi, O. M. (2018). Evaluation of anti-obesity and lipid-lowering properties of Vaccinium myrtillus leaves powder extract in a hamster model. Journal of basic and clinical physiology and pharmacology, 29(6), 697-703. https://doi.org/10.1515/jbcpp-2017-0161

Koshovyi, O., Granica, S., Piwowarski, J. P., Stremoukhov, O., Kostenko, Y., Kravchenko, G., Krasilnikova, O., & Zagayko, A. (2021). Highbush Blueberry (Vaccinium corymbosum L.) Leaves Extract and Its Modified Arginine Preparation for the Management of Metabolic Syndrome-Chemical Analysis and Bioactivity in Rat Model. Nutrients, 13(8), 2870. https://doi.org/10.3390/nu13082870

Chaika, N., Mazen, M., Koshovyi, O., Kravchenko, G., Goryacha, O., Kireyev, I., Kovalenko, S., & Darmograi, R. (2021). Research in phytochemical composition and hypoglycemic activity screening of the dry extracts from bearberry leaves. ScienceRise: Pharmaceutical Science, 31(3), 42-50. https://doi.org/10.15587/2519-4852.2021.235939

Chaika, N., Koshovyi, O., Raal, A., Kireyev, I., Zupanets, A., & Odyntsova, V. (2020). Phytochemical profile and pharmacological activity of the dry extract from arctostaphylos uva-ursi leaves modified with phenylalanine. ScienceRise: Pharmaceutical Science, 28(6), 74-84. https://doi.org/10.15587/2519-4852.2020.222511

Koshovyi, O. M., Vlasova, I. K., Briukhanova, T. O., Briukhanova, T. O., Krasilnikova, O. A., Kravchenko, H. B., Zahaiko, A. L., & Komisarenko, M. A. (2021). Sposib oderzhannia likuvalno-profilaktychnoho zasobu z lystia zhuravlyny velykoplodoi dlia korektsii insulinorezystentnykh staniv [A method of obtaining a therapeutic and prophylactic agent from the leaves of large-fruited cranberries for the correction of insulin-resistant conditions]. Ukraine Patent UA 147975. https://sis.ukrpatent.org/uk/search/detail/1601046/

Zhuravlyna dribnoploda. Oxycoccus microcarpus Turcz. ex Rupr. [Small cranberry. Oxycoccus microcarpus Turcz. ex Rupr.]. Chervona knyha Ukrainy. http://redbook-ua.org/item/oxycoccus-microcarpus-turcz-ex-ruprschmalh-v-oxycoccus-l-subsp-micro/

Ministry of Health of Ukraine. (2012). Likarski zasoby. Nalezhna praktyka kultyvuvannia ta zbyrannia vykhidnoi syrovyny roslynnoho pokhodzhennia [Good practice of cultivation and collection of raw materials of plant origin]. Guidelines ST-N MOZU 42-4.5:2012. https://zakon.rada.gov.ua/rada/file/text/23/f309869n4766.pdf

Konovalchuk, V. K. (2002). Vyvchennia sortiv zhuravlyny velykoplidnoi, lokhyny vysokorosloi i brusnytsi ta zony yikh vyroshchuvannia v Ukraini [Research of the varieties of large-fruited cranberry, highbush blueberry and lingonberry, and the areas of their growing in Ukraine]. Naukovyi visnyk, (12.4), 229-233. [in Ukrainian].

Prokudin, Y. N., Dobrochaeva, D. N., Zaverukha, B. V., & Chopik, V. I. (1999). Opredelitel vysshikh rastenii Ukrainy [Key to Higher Plants of Ukraine] (2nd ed.). Kiev: Fitosotsiotsentr. [in Russian].

State Enterprise Ukrainian Scientific Pharmacopoeial Center of Medicines Quality. (2015). Derzhavna Farmakopeya Ukrayiny [The State Pharmacopoeia of Ukraine] (Vol. 1, 2nd ed.). Kharkiv: State Enterprise Ukrainian Scientific Pharmacopoeial Center of Medicines Quality. [in Ukrainian].

Koshovyi, O. M. (2012). Fenolnyi sklad deiakykh predstavnykiv pidrodu Sclarea rodu Salvia [Phenolic composition of some members of the subgenus Sclarea genus Salvia]. Current issues in pharmacy and medicine: science and practice, (3), 11-14. [in Ukrainian].

Koshoviy, O. M. (2013). Suchasni pidkhody do stvorennia likarskykh zasobiv na osnovi roslyn rodiv Evkalipt ta Shavliia [Contemporary approaches to the new remedies on the basis of the plants from Eucalyptus and Salvia genera creation (Abstract for Doctoral Dissertation)]. The National University of Pharmacy, Kharkiv, Ukraine.

Koshovyi, O., Romanenko, Ye., & Komissarenko, A. (2016). The study of the phenolic composition of the dry extract of motherwort herb and its psychotropic activity. American Journal of Science and Technologies, 1(21), 1055-1059.

Koshovyy, O. M., Perederiy, Ye. O., Osolodchenko, T. P., Kovalova, A. M., & Komisarenko, A. M. (2011). Doslidzhennia izoprenoidnoho skladu ta antymikrobnoi aktyvnosti hustoho ekstraktu lystia shavlii likarskoi [Investigation of isoprenoid composition and antimicrobial activity of thick sage leaf extract]. Klinichna farmatsiia, 15(1), 26-29. [in Ukrainian].

Koshovyi, O. M., Kukhtenko, O. S., Kovalova, A. M., Komisarenko, A. M., Vynnyk, O. V., & Sholom, Yu. H. (2010). Optymizatsiia protsesu ekstraktsii biolohichno aktyvnykh rechovyn lystia evkalipta: kratnist ekstraktsii [Optimization of the extraction process of biologically active substances of eucalyptus leaves: multiplicity of extraction]. Current issues in pharmacy and medicine: science and practice, 23(1), 47-49. [in Ukrainian].

Published

2022-08-01

How to Cite

1.
Vlasova IK, Koshovyi OM, Kukhtenko OS, Komisarenko MA, Ilina ТV, Kovalyova AM. Determination of biologically active substances extraction parameters from cranberry leaves. Current issues in pharmacy and medicine: science and practice [Internet]. 2022Aug.1 [cited 2024Oct.12];15(2):145-52. Available from: http://pharmed.zsmu.edu.ua/article/view/258524

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Section

Original research