The effect of immunomodulator azoximer bromide on immunoglobulin levels in children with newly diagnosed tuberculosis

Authors

DOI:

https://doi.org/10.14739/2409-2932.2022.2.255746

Keywords:

children, tuberculosis, immunoglobulins, treatment

Abstract

Aim. To analyze the effect of the immunomodulator azoximer bromide on serum immunoglobulins (IgE, A, M, G) in the complex treatment for children with newly diagnosed tuberculosis.

Materials and methods. The study was included 51 with newly diagnosed tuberculosis children, which were divided into 2 groups: 26 children suffer from disease, who received immunomodulator azoximer bromide (main group) in combination treatment with antimycobacterial therapy (AMBT) and 25 children suffer from disease who received AMBT only (control group). The comparison group included 30 healthy children. The children from the main group on the background of standard AMBT additionally were used the azoximer bromide (immunomodulator) to correct immunological changes: in children under 10 years of age, 6 mg twice a day, in children over 10 years – 12 mg twice a day; course of treatment – 14 days. Research indicators such as IgE, A, M, G in serum were performed on the basis of the Immunological Department in the Training Medical and Laboratory center of ZSMU by solid-phase enzyme-linked immunosorbent assay (ELISA) using a device enzyme-linked immunosorbent reader Sirio S using reagent kits LTD RPL “Granum” (Kharkiv), units of measurement: IgE – IU/ml), IgA, M, G – g/l. The study was conducted at the beginning of intensive phase (IF) AMBT and at the end of the AMBT maintenance phase (MF). The study results were processed on a personal computer using the statistical package of the licensed program Statistica, version 13 (Copyright 1984–2018 TIBCO Software Inc. All rights reserved, License No. JPZ8041382130ARCN10-J).

Results. Children with newly diagnosed tuberculosis, who received immunomodulator azoximer bromide in combination therapy, after the end of the AMBT MF was determined by the normalization of serum IgA levels (1.22 (0.75; 1.74) g/l against 1.70 (1.11; 2.01) g/l, P < 0.02), which was 1.4 times lower than children who received only AMBT. The levels of IgE and M throughout the course of treatment were within the age norm with decreasing at the end of treatment, and the level of IgG throughout the course of treatment remained significantly higher than that of the healthy children group. Children with newly detected tuberculosis who received only AMBT, after the end of AMBT MF on the background of normal levels of IgE and M, the level of IgA remained significantly higher in 1.4 times, and the level of IgG tended to increase.

Conclusions. Considering the obtained results, children with newly detected tuberculosis, who received of the immunomodulator azoximer bromide in complex treatment, helped to normalize of IgA level in serum, after completion of treatment. Therefore, the effect of the immunomodulator azoximer bromide in the complex therapy of children with newly detected tuberculosis on immunoglobulin indicators were significant, which would improve the effectiveness of antimycobacterial therapy in this contingent of patients in general.

Author Biographies

O. M. Raznatovska, Zaporizhzhia State Medical University, Ukraine

MD, PhD, DSc, Professor, Head of the Department of Phthisiology and Pulmonology

Yu. V. Myronchuk, Zaporizhzhia State Medical University, Ukraine

Assistant of the Department of Phthisiology and Pulmonology

References

Bakker M. & Wicherts J. M. (2014). Outlier removal, sum scores, and the inflation of the Type I error rate in independent samples t tests: the power of alternatives and recommendations. Psychol Methods, 19(3), 409–427. https://doi.org/10.1037/met0000014.

Bishara A. J. & Hittner J. B. (2012). Testing the significance of a correlation with nonnormal data: comparison of Pearson, Spearman, transformation, and resampling approaches. Psychol Methods, 17(3), 399–417. https://doi.org/10.1037/a0028087.

Robertson B. D., Altmann D., Barry C., Bishai B., Cole S., Dick T. et al. (2012). Detection and treatment of subclinical tuberculosis. Tuberculosis (Edinb), 92(6), 447–452. https://doi.org/10.1016/j.tube.2012.06.004

Abebe F., Belay M., Legesse M. & Franken K. M. C. (2018). IgA and IgG against Mycobacterium tuberculosis Rv2031 discriminate between pulmonary tuberculosis patients, Mycobacterium tuberculosis-infected and non-infected individuals. PLoS One, 13(1), e0190989. https://doi.org/10.1371/journal.pone.0190989

Le Boedec K. (2016). Sensitivity and specificity of normality tests and consequences on reference interval accuracy at small sample size: a computer-simulation study. Vet Clin Pathol, 45(4), 648–656. https://doi.org/10.1111/vcp.12390

Mironchuk Yu. V. & Raznatovska O. M. (2022). The effect of immunomodulator azoximer bromide on the cytokine profile in a complex therapy for children with newly diagnosed tuberculosis. Zaporozhye Medical Journal, 2 (131), 187–190. https://doi.org/10.14739/2310-1210.2022.2.249347

Araujo Z., Giampietro F., Rivas-Santiago B., Luna-Herrera J., Wide A., Clark W. et al. (2012). Patients exposed to Mycobacterium tuberculosis infection with a prominent IgE response. Arch Med Res, 43(3), 225–232. https://doi.org/10.1016/j.arcmed.2012.04.002

Kunnath-Velayudhan S., Davidow A. L., Wang H. Y., Molina D. M., Huynh V. T., Salamon H. et al. (2012). Proteome-scale antibody responses and outcome of Mycobacterium tuberculosis infection in nonhuman primates and in tuberculosis patients. J Infect Dis, 206(5), 697–705. https://doi.org/10.1093/infdis/jis421

Raznatovska O. M., Mironchuk Yu. V. & Lytvynenko O. S. (2018). Levels of E, A, M, G immunoglobulins among children diagnosed with tuberculosis at the beginning of antimycobacterial therapy. Запорожский медицинский журнал, 1 (106), 86–90. https://doi.org/10.14739/2310-1210. 2018.1.122003

Rohini K., Srikumar P. S. & Mahesh Kumar A. A. (2012). Study on the Serum Immunoglobulin Levels in Pulmonary Tuberculosis Patients. IJBBB, 2(4), 280–281. https://doi.org/10.7763/IJBBB.2012.V2.116

Schroeder H. W. Jr. & Cavacini L. (2010). Structure and function of immunoglobulins. J Allergy Clin Immunol, 125(2 Suppl 2), 41–52. https://doi.org/10.1016/j.jaci.2009.09.046

Olivares N., Marquina B., Mata-Espinoza D., Zatarain-Barron Z. L., Pinzón C. E,. Estrada I. et al. (2013). The protective effect of immunoglobulin in murine tuberculosis is dependent on IgG glycosylation. Pathog Dis, 69(3), 176–183. https://doi.org/10.1111/2049-632X.12069

Thomas T. A. (2017). Tuberculosis in children. Pediatr Clin North Am, 64(4), 893–909. https://doi.org/10.1016/j.pcl.2017.03.010

Vetter T. R. (2017). Descriptive Statistics: Reporting the Answers to the 5 Basic Questions of Who, What, Why, When, Where, and a Sixth, So What? Anesth Analg, 125(5), 1797–1802. https://doi.org/10.1213/ANE.0000000000002471

Published

2022-08-01

How to Cite

1.
Raznatovska OM, Myronchuk YV. The effect of immunomodulator azoximer bromide on immunoglobulin levels in children with newly diagnosed tuberculosis. Current issues in pharmacy and medicine: science and practice [Internet]. 2022Aug.1 [cited 2024Oct.12];15(2):174-8. Available from: http://pharmed.zsmu.edu.ua/article/view/255746

Issue

Section

Original research