Ultrastructural changes in renal tissue in rats with renal failure under the influence of herbal composition BNO 2103
DOI:
https://doi.org/10.14739/2409-2932.2022.2.251800Keywords:
BNO 2103, herbal composition, chronic kidney disease, nephroprotective effect, phytotherapy, preclinical studyAbstract
Management of chronic kidney disease is an urgent task of the global health care system, as untreated, it leads to partial or permanent loss of kidney function. Standardized herbal remedies are highly effective and relatively safe. That is why it is suggested to pay attention to herbal remedies already known for the treatment of genitourinary diseases to expand the indications for their use.
The aim of the research is to study the nephroprotective properties of the standardized herbal composition BNO 2103, which is the active ingredient of Canephron N ®, in a model of renal failure in rats.
Materials and methods. An experimental study was performed on 42 male white outbred rats weighing 150–200 g, which were divided into 4 groups. Nephropathy was induced by a single subcutaneous injection of 0.7 ml/kg of 2.5 % potassium chromate solution. Animals in 3 and 4 groups received BNO 2103 at a dose of 33.0 mg/kg and prednisolone at a dose of 1.9 mg/kg intragastrically daily for 20 days. After animals were sacrificed, their kidneys were obtained and studied with standard electronic microscopy methods.
Results. BNO 2103 normalized the ultrastructural picture of the kidneys, however, with the presence of minor pathological signs. BNO 2103 reduced the destruction of basement membranes, improved organelle structure and increased metabolic processes in podocytes, and endotheliocytes, reducing the manifestations of degenerative-dystrophic changes in them. BNO 2103 was superior to the reference drug prednisolone.
Conclusions. BNO 2103 showed a significant nephroprotective effect, normalizing the ultrastructural picture of the kidneys in rats with renal failure. This effect is most likely related to the presence of biologically active substances with a powerful antioxidant, anti-inflammatory, nephroprotective activity. The obtained results allow us to consider BNO 2103 as a promising nephroprotective agent for the treatment of chronic kidney disease.
References
GBD Chronic Kidney Disease Collaboration (2020). Global, regional, and national burden of chronic kidney disease, 1990-2017: a systematic analysis for the Global Burden of Disease Study 2017. Lancet, 395(10225), 709-733. https://doi:10.1016/S0140-6736(20)30045-3
Höller, M., Steindl, H., Abramov-Sommariva, D., Wagenlehner, F., Naber, K. G., & Kostev, K. (2021). Treatment of Urinary Tract Infections with Canephron® in Germany: A Retrospective Database Analysis. Antibiotics, 10(6), 685. https://doi.org/10.3390/antibiotics10060685
Shebeko, S. K., Chernykh, V. V., & Zupanets, K. O. (2020). Nephroprotective Effect of the Herbal Composition BNO 2103 in Rats with Renal Failure. Scientia Pharmaceutica, 88(4), 1-12. https://doi.org/10.3390/scipharm88040047
Faith, R. E., Allen, K. P., & Hessler, J. R. (2020). The Laboratory Rat (3rd ed., pp. 350-381). Elsevier. https://doi.org/10.1016/b978-0-12-814338-4.00010-6
Parliament, European & Council, European. (2010). Directive 2010/63/EU on the protection of animals used for scientific purposes. EU Official Journal. L276, 33-79. http://data.europa.eu/eli/dir/2010/63/2019-06-26
Appel, G. B., & D’Agati, V. D. (2019). Primary and Secondary (Non-Genetic) Causes of Focal and Segmental Glomerulosclerosis. In J. Feehally, J. Floege, M. Tonelli, R. J. Johnson (Eds.), Comprehensive Clinical Nephrology (6th ed., pp. 219-231). Elsevier.
Shebeko, S., Zupanets, I., & Zimina, M. (2020). Dose-dependent efficacy of the N-acetylglucosamine and quercetin combination in rats with renal failure. Acta Pharmaceutica Sciencia, 58(2), 154-168. http://doi:
Wedeen, R. P., & Qian, L. F. (1991). Chromium-induced kidney disease. Environmental health perspectives, 92, 71-74. https://doi.org/10.1289/ehp.92-1519395
Center for Drug Evaluation and Research. (2005, July 6). Estimating the Maximum Safe Starting Dose in Initial Clinical Trials for Therapeutics in Adult Healthy Volunteers. U.S. Food and Drug Administration. https://www.fda.gov/regulatory-information/search-fda-guidance-documents/estimating-maximum-safe-starting-dose-initial-clinical-trials-therapeutics-adult-healthy-volunteers
Flecknell, P. A. (2015). Laboratory Animal Anesthesia (4th ed.). Academic Press.
Exbrayat, J. M. (2013). General techniques in electron microscopy. In J. M. Exbrayat (Ed.), Histochemical and cytochemical methods of visualization (pp. 223-236). CRC Press.
Bozzola, J. J. (2014). Conventional specimen preparation techniques for scanning electron microscopy of biological specimens. In J. Kuo (Ed.), Electron microscopy: methods and protocols. Methods in molecular biology (Vol. 1117, pp. 133-150). Humana Press.
Hendricks, G. M. (2014). Metal shadowing for electron microscopy. In J. Kuo (Ed.), Electron microscopy: methods and protocols. Methods in molecular biology (Vol. 1117, pp. 73-93). Humana Press.
Islam, M. A., & Al-Shiha, A. (2018). Foundations of Biostatistics (1st ed., pp. 39-46; 346-368). Springer Singapore. https://doi.org/10.1007/978-981-10-8627-4
Mescher, A. L. (2018). Junqueira’s basic histology : text and atlas (15th ed.). Mcgraw-Hill Medical.
Begum, A., Sandhya, S., Shaffath Ali, S., Vinod, K. R., Reddy, S., & Banji, D. (2013). An in-depth review on the medicinal flora Rosmarinus officinalis (Lamiaceae). Acta scientiarum polonorum. Technologia alimentaria, 12(1), 61-73.
El-Desouky, M. A., Mahmoud, M. H., Riad, B. Y., & Taha, Y. M. (2019). Nephroprotective effect of green tea, rosmarinic acid and rosemary on N-diethylnitrosamine initiated and ferric nitrilotriacetate promoted acute renal toxicity in Wistar rats. Interdisciplinary toxicology, 12(2), 98-110. https://doi.org/10.2478/intox-2019-0012
Samsu, N., Soeharto, S., Rifai, M., & Rudijanto, A. (2019). Rosmarinic acid monotherapy is better than the combination of rosmarinic acid and telmisartan in preventing podocyte detachment and inhibiting the progression of diabetic nephropathy in rats. Biologics : targets & therapy, 13, 179-190. https://doi.org/10.2147/BTT.S214820
Inyushkina, Y. V., Bulgakov, V. P., Veselova, M. V., Bryukhanov, V. M., Zverev, Y. F., Lampatov, V. V., Azarova, O. V., Tchernoded, G. K., Fedoreyev, S. A., & Zhuravlev, Y. N. (2007). High rabdosiin and rosmarinic acid production in Eritrichium sericeum callus cultures and the effect of the calli on masugi-nephritis in rats. Bioscience, biotechnology, and biochemistry, 71(5), 1286-1293. https://doi.org/10.1271/bbb.60684
Ozturk, H., Ozturk, H., Terzi, E. H., Ozgen, U., Duran, A., & Uygun, I. (2014). Protective effects of rosmarinic acid against renal ischaemia/reperfusion injury in rats. JPMA. The Journal of the Pakistan Medical Association, 64(3), 260-265.
Tavafi, M., & Ahmadvand, H. (2011). Effect of rosmarinic acid on inhibition of gentamicin induced nephrotoxicity in rats. Tissue and Cell, 43(6), 392-397. https://doi.org/10.1016/j.tice.2011.09.001
Miran, M., Monsef Esfahani, H., Jung, J. H., Aliahmadi, A., Skropeta, D., Abbas-Mohammadi, M., Nejad Ebrahimi, S., & Moridi Farimani, M. (2020). Characterization and Antibacterial Activity of Phthalides from the Roots of the Medicinal Herb Levisticum officinale W.D.J. Koch. Iranian journal of pharmaceutical research : IJPR, 19(2), 182-186. https://doi.org/10.22037/ijpr.2020.112583.13839
Xu, F., Fang, X., Ye, Z., & Tao, S. (2020). Ligustilide alleviates podocyte injury via suppressing the SIRT1/NF-κB signaling pathways in rats with diabetic nephropathy. Annals of Translational Medicine, 8(18), 1154-1154. https://doi.org/10.21037/atm-20-5811
Ghaedi, N., Pouraboli, I., & Askari, N. (2020). Antidiabetic Properties of Hydroalcoholic Leaf and Stem Extract of Levisticum officinale: An implication for α-amylase Inhibitory Activity of Extract Ingredients through Molecular Docking. Iranian journal of pharmaceutical research : IJPR, 19(1), 231-250. https://doi.org/10.22037/ijpr.2020.15140.12901
Migliori, M., Cantaluppi, V., Mannari, C., Bertelli, A. A., Medica, D., Quercia, A. D., Navarro, V., Scatena, A., Giovannini, L., Biancone, L., & Panichi, V. (2015). Caffeic acid, a phenol found in white wine, modulates endothelial nitric oxide production and protects from oxidative stress-associated endothelial cell injury. PloS one, 10(4), e0117530. https://doi.org/10.1371/journal.pone.0117530
Rai, U., Kosuru, R., Prakash, S., Tiwari, V., & Singh, S. (2019). Tetramethylpyrazine alleviates diabetic nephropathy through the activation of Akt signalling pathway in rats. European Journal of Pharmacology, 865, 172763. https://doi.org/10.1016/j.ejphar.2019.172763
Downloads
Published
How to Cite
Issue
Section
License
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access)
