APPLICATION OF UV-SPECTROPHOTOMETRY FOR THE QUANTITATIVE DETERMINATION OF CAPTOPRIL IN DRUG FORMULATIONS

Authors

  • Yu. V. Monaykina Zaporozhye State Medical University,
  • S. O. Vasjuk Zaporozhye State Medical University,
  • D. M. Romanina Zaporozhye State Medical University,
  • I. O. Puhalska Zaporozhye State Medical University,

DOI:

https://doi.org/10.14739/2409-2932.2014.1.24836

Keywords:

spectrophotometrу, validation, quantitative determination, praziquantel, dosage form

Abstract

Introduction. In this paper we have developed the simple, rapid and sensitive assay method for the quantitative determination of praziquantel which is an anthelmintic with a broad spectrum of activity. Analysis has been performed directly by using zero-order UV-spectrophotometry. It is known from the special literature that the chromatographic techniques used for praziquantel assay require expensive equipment and are rather time consuming. Therefore UV-spectrophotometry is preferable due to its accuracy, sensitivity and simplicity comparing to other methods.

The aim of this study was to develop and validate a new, simple, rapid, precise and accurate UV-spectrophotometric method for the quantitative determination of praziquantel in a tablet formulation.

Materials and methods. The object of the study was the drug formulation of praziquantel – «Biltricid» 600 mg tablets (Bayer Schering Pharma AG). 70% ethanol was used as the solvent, working standard sample of praziquantel was used as a reference standard.

Analytical equipment: spectrophotometer Specord 200, electronic balance ABT-120-5DM, measuring glassware of class A.

Assay procedure: An accurately weighed sample of the powdered tablets (0,0152 –0,0274 g) was put into a 50,00 ml volumetric flask, brought to the mark with 70% ethanol, stirred and filtrated. 1,00 ml of the resulting solution was transferred into a 25,00 ml volumetric flask and completed to the mark with the same solvent. Absorbance was measured at a wavelength of 210nm. The parallel measurement with 1,00 ml of 0,02% praziquantel standard solution was carried out. The content of active substance was calculated according to a standard formula.

Results. The suggested procedure has been successfully applied for the analysis of a pharmaceutical dosage form. The results obtained by applying the proposed method have been statistically analyzed. Validation of the method confirmed its proper precision (100,3%, RSD% = 0,531, n = 9), recovery (100,1%, n = 9) and linearity (0,80–1,44 μg/ml, r = 0,9999, n=5) The received parameters enable the developed procedure to be used in quantitative pharmaceutical analysis.

Conclusions. The applicability of the new procedure for routine quality control is well established by the assay of praziquantel in pure form, as well as in pharmaceutical preparation. The developed UV-spectrophotometric method is potentially useful because of its simplicity, rapidity and accuracy and is valid according to the validation requirements of Ukrainian Pharmacopeia.

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How to Cite

1.
Monaykina YV, Vasjuk SO, Romanina DM, Puhalska IO. APPLICATION OF UV-SPECTROPHOTOMETRY FOR THE QUANTITATIVE DETERMINATION OF CAPTOPRIL IN DRUG FORMULATIONS. Current issues in pharmacy and medicine: science and practice [Internet]. 2014May23 [cited 2024Dec.23];(1). Available from: http://pharmed.zsmu.edu.ua/article/view/24836

Issue

No. 1 (2014)

Section

Analysis and standardization of biologycally active substances and dosage forms

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