The microscopic diagnostic signs of some Fabaceae L. genus representatives. Message ІІ. Plant conducting system

Authors

DOI:

https://doi.org/10.14739/2409-2932.2021.3.234470

Keywords:

microscopy, identity, raw materials, conducting plant system

Abstract

The world plant products market is expanded rapidly and trade in them tends to grow by 15–25 % annually. The number of reports is increased proportionally about accidental contamination or deliberate, economically motivated falsification of plant raw materials. 27 % of the nearly 6.000 herbal preparations that are sold in 37 countries have contained undeclared contaminants, substitutes, or other components, according to the literature. We have conducted a plant conduction system microscopic analysis of the individual members’ genus Clover (Trifoliae L.), Fabaceae L. to identify morphoanatomical characteristics. Clover has anti-inflammatory, antiseptic, choleretic, diaphoretic, diuretic, hemostatic, expectorant, astringent properties and is used in many diseases.

Aim. The finding common features and those that differ and can be used as diagnostic during studying the conducting system structure of genus Trifolium L. leaves and stems.

Materials and methods. Plant material (herb) from Trifolium pratense L., T. incarnatum L., T. repens L. and T. fragiferum L. was harvested during the active flowering period – (May – June) and was dried in a well-ventilated place. Leaves and stems preparations were pre-boiled in 5 % sodium hydroxide water solution and fixed in chloral hydrate solution. Cross-sections were made with a microtome. The BIOLAM LOMO light microscope (Russia) and OLYMPUS SH-21 digital camera were used to record the data about identify the conducting apparatus of the plant’s leaf, petiole, and stem.

Results. It has been examined the central vein structure of T. pratense L. and T. fragiferum L. leaves, it was determined that the conductive system is covered with a crystalline coating and there is one closed collateral bundle in the center, which is not typical for dicotyledonous plants. The petioles of T. incarnatum L., T. fragiferum L., and T. repens L. in cross-section are several different shapes. There are kidney-shaped and round. The conducting apparatus T. incarnatum L. and T. repens L. have arranged in a circle, closed and collateral. The leafstalk structure type of T. fragiferum L. is bunchles. It contradicts too the information about the structure conducting system of dicotyledonous plants. The stem’s conducting bundles are collateral and open.

Conclusions. We have paid attention to the structural peculiarities of the conductive system of the central vein and petiole of objects for study: Trifolium pratense L., T. incarnatum L., T. repens L., and T. fragiferum L. when searching for differentiating features in some members of the genus Trifoliae L. in pharmacognostic analysis. The Dicotyledonae representatives are not characteristic of the closed type of conductive bundles, which we observed during microscopic examination.

Author Biographies

O. V. Grechana, Zaporizhzhia State Medical University, Ukraine

PhD, Associate Professor of the Department of Pharmacognosy, Pharmacology and Botany

A. H. Serbin, National University of Pharmacy, Kharkiv, Ukraine

PhD, DSc, Professor of the Department of Botany

A. M. Rudnyk, Zaporizhzhia State Medical University, Ukraine

PhD, Associate Professor of the Department of Pharmacognosy, Pharmacology and Botany

O. O. Salii, Kyiv National University of Technologies and Design, Ukraine

PhD, Associate Professor of the Department of Industrial Pharmacy

References

Grazina, L., Amaral, J. S., & Mafra, І. (2020). Botanical origin authentication of dietary supplements by DNA-based approaches. Comprehensive Reviews in Food Science and Food Safety, 19(3), 1080-1109. https://doi.org/10.1111/1541-4337.12551

Michetti, K. M., Pérez Cuadra, V., & Cambi, V. N. (2019). Botanical quality control of digestive tisanes commercialized in an urban area (Bahía Blanca, Argentina). Revista Brasileira de Farmacognosia, 29(2), 137-146. https://doi.org/10.1016/j.bjp.2019.01.002

Pawar, R. S., Handy, S. M., Cheng, R., Shyong, N., & Grundel, E. (2017). Assessment of the Authenticity of Herbal Dietary Supplements: Comparison of Chemical and DNA Barcoding Methods. Planta Medica, 83(11), 921-936. https://doi.org/10.1055/s-0043-107881

Ghosh, D. (2018). Quality Іssues of Herbal Medicines: Internal and Еxternal Factors. International Journal of Complementary & Alternative Medicine, 11(1), 67-69. https://doi.org/10.15406/ijcam.2018.11.00350

Simmler, C., Graham, J. G., Chen, S. N., & Pauli, G. F. (2018). Integrated analytical assets aid botanical authenticity and adulteration management. Fitoterapia, 129, 401-414. https://doi.org/10.1016/j.fitote.2017.11.017

Grechana, О. V., Serbin, A. G., Trshecinskiy, S. D., Panasenko, O. I., Klimenko, L. Y., Oproshanska, T. V., & Saliy, O. O. (2020). Some questions about teae folia (Thea sinensis l. seu camellia sinensis l. kuntze) as а medicinal raw material. EurAsian Journal of BioSciences, 14(2), 2569-2575.

Karakaya, S., Polat, A., Aksakal, Ö., Sümbüllü, Y. Z., & İncekara, Ü. (2020). Ethnobotanical study of medicinal plants in aziziye district (Erzurum, Turkey). Turkish Journal of Pharmaceutical Sciences, 17(2), 211-220. https://doi.org/10.4274/tjps.galenos.2019.24392

Ichim, M. C. (2019). The DNA-based authentication of commercial herbal products reveals their globally widespread adulteration. Frontiers in Pharmacology, 10, 1227. https://doi.org/10.3389/fphar.2019.01227

Ichim, M. C., Häser, A., & Nick, P. (2020). Microscopic Authentication of Commercial Herbal Products in the Globalized Market: Potential and Limitations. Frontiers in Pharmacology, 11, 876. https://doi.org/10.3389/fphar.2020.00876

Muzashvili, T., Moniuszko-Szajwaj, B., Pecio, L., Oleszek, W., & Stochmal, A. (2014). Ultraperformance liquid chromatography tandem mass spectrometry determination of cyanogenic glucosides in Trifolium species. Journal of agricultural and food chemistry, 62(8), 1777-1782. https://doi.org/10.1021/jf4056659

Zareh, M., Faried, A., & Farghaly, N. (2017). Micromorphological studies on the genus Lotus L. (Fabaceae: Loteae) from Egypt. Turkish Journal of Botany, 41(3), 273-288. https://doi.org/10.3906/bot-1607-48

Wu, X., Wang, S., Lu, J., Jing, Y., Li, M., Cao, J., Bian, B., & Hu, C. (2018). Seeing the unseen of Chinese herbal medicine processing (Paozhi): advances in new perspectives. Chinese medicine, 13, 4. https://doi.org/10.1186/s13020-018-0163-3

Shaheen, S., Fateh, R., Younis, S., Harun, N., Jaffer, M., Hussain, K., Ashfaq, M., Siddique, R., Mukhtar, H., & Khan, F. (2020). Light and scanning electron microscopic characterization of thirty endemic Fabaceae species of district Lahore, Pakistan. Microscopy research and technique, 83(12), 1507-1529. https://doi.org/10.1002/jemt.23545

Abusaief, H., & Boasoul, S. H. (2021). A taxonomic study of twelve wild forage species of Fabaceae. Heliyon, 7(2), e06077. https://doi.org/10.1016/j.heliyon.2021.e06077

Grosu, E., & Ichim, M. C. (2020). Turning Meadow Weeds Into Valuable Species for the Romanian Ethnomedicine While Complying With the Environmentally Friendly Farming Requirements of the European Union's Common Agricultural Policy. Frontiers in pharmacology, 11, 529. https://doi.org/10.3389/fphar.2020.00529

Published

2021-10-25

How to Cite

1.
Grechana OV, Serbin AH, Rudnyk AM, Salii OO. The microscopic diagnostic signs of some Fabaceae L. genus representatives. Message ІІ. Plant conducting system. Current issues in pharmacy and medicine: science and practice [Internet]. 2021Oct.25 [cited 2024Nov.14];14(3):292-8. Available from: http://pharmed.zsmu.edu.ua/article/view/234470

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Section

Original research