Chromato-mass-spectroscopic research of chemical composition of Elaeagnus angustifolia L.
Keywords:Elaeagnus angustifolia, GS-MS, antimicrobial action, antidiabetic action, cytotoxic action, anti-inflammatory action, biological activity
The aim of the work was chromato-mass-spectroscopic research of chemical composition of Elaeagnus angustifolia L. fruits and leaves and identifying further prospects for the use of this plant in medicine.
Materials and methods. Raw materials of Elaeagnus angustifolia L. were selected as objects of study. The tincture was obtained by maceration and the raw material was extracted with methyl alcohol at room temperature for 10 days according to the method of making tinctures. The study of the chemical composition of Elaeagnus angustifolia L. was carried out using gas chromatograph Agilent 7890B GC System (Agilent, Santa Clara, CA, USA) with mass spectrometric detector Agilent 5977 BGC/MSD (Agilent, Santa Clara, CA, USA) and chromatographic column DB-5ms (30 m × 250 mkm × 0,25 mkm).
Results. 23 compounds (1 in the isomeric state) in fruits and 20 compounds (2 in the isomeric state) in the leaf of Elaeagnus angustifolia L. were identified. The main components of fruits were sitosterol (phytosterols) – 12.53 %, propyl acetate (esters of carboxylic acids) – 12.60 %, chamazulene (terpenes) – 11.97 % and palmitic acid (fatty acids) – 8.28 %. The main component of leaves were sitosterol (phytosterols) – 17.57 %, 1-(2-hydroxy-5-methylphenyl)-ethanone (ketone) – 8.35 %, phytol (terpenes) – 6.10 %.
It is known from the literature that chamazulene has antioxidant, antinociceptive, cytotoxic activity. Sitosterol has anti-inflammatory and antidiabetic activity. Hexadecanoic (palmitic) acid has antimicrobial, antidiabetic and antioxidant properties. Phytol is characterized by a wide range of biological action – antimicrobial, antinociceptive, anti-inflammatory, antioxidant and cytotoxic.
Conclusions. Based on the above, the olive can be considered as a source of antimicrobial, antinociceptive, anti-inflammatory, antioxidant, antidiabetic and cytotoxic drugs.
Elaeagnus (n.d.). The Plant List. http://www.theplantlist.org/tpl1.1/search?q=Elaeagnus
Khadivi, A. (2018). Phenotypic characterization of Elaeagnus angustifolia using multivariate analysis. Industrial Crops and Products, 120, 155-161. https://doi.org/10.1016/j.indcrop.2018.04.050
Nazir, N., Zahoor, M. & Nisar, M. (2020). A Review on Traditional Uses and Pharmacological Importance of Genus Elaeagnus Species. The Botanical Review, 86, 247-280. https://doi.org/10.1007/s12229-020-09226-y
Panahi, Y., Alishiri, G. H., Bayat, N., Hosseini, S. M., & Sahebkar, A. (2016). Efficacy of Elaeagnus Angustifolia extract in the treatment of knee osteoarthritis: a randomized controlled trial. EXCLI journal, 15, 203-210. https://doi.org/10.17179/excli2015-639
Mahboubi, M. (2018). Elaeagnus angustifolia and its therapeutic applications in osteoarthritis. Industrial Crops and Products, 121, 36-45. https://doi.org/10.1016/j.indcrop.2018.04.051
Niknam, F., Azadi, A., Barzegar, A., Faridi, P., Tanideh, N., & Zarshenas, M. M. (2016). Phytochemistry and Phytotherapeutic Aspects of Elaeagnus angustifolia L. Current drug discovery technologies, 13(4), 199-210. https://doi.org/10.2174/1570163813666160905115325
Hamidpour, R., Hamidpour, S., Hamidpour, M., Shahlari, M., Sohraby, M., Shahlari, N., & Hamidpour, R. (2017). Russian olive (Elaeagnus angustifolia L.): From a variety of traditional medicinal applications to its novel roles as active antioxidant, anti-inflammatory, anti-mutagenic and analgesic agent. Journal of Traditional and Complementary Medicine, 7(1), 24-29. https://doi.org/10.1016/j.jtcme.2015.09.004
Sabouri, S., Rad, A. H., Peighambardoust, S. H., Fathipour, R. B., Feshangchi, J., Ansari, F., & Pourjafar, H. (2021). The Oleaster (Elaeagnus angustifolia): A Comprehensive Review on Its Composition, Ethnobotanical and Prebiotic Values. Current pharmaceutical biotechnology, 22(3), 367-379. https://doi.org/10.2174/1389201020666191107112243
State Enterprise Ukrainian Scientific Pharmacopoeial Center of Medicines Quality. (2008, February 1). Derzhavna Farmakopeia Ukrainy. Dopovnennia 2 [The State Pharmacopoeia of Ukraine (1st ed., Suppl. 1)]. Kharkiv: Naukovo-ekspertnyi farmakopeinyi tsentr. [in Ukrainian].
Noori, K., Omidi, H., & Pirahmadi, L. (2016). Morphological characteristics, essential oil, chamazulene percentage and anti-oxidation enzymes activity changes of chamomile Matricaria recutita L. under the soil and water salinity. Journal of Fundamental and Applied Sciences, 8(2S), 2293-2310.
Reis Simas, D. L., Mérida-Reyes, M. S., Muñoz-Wug, M. A., Cordeiro, M. S., Giorno, T., Taracena, E. A., Oliva-Hernández, B. E., Martínez-Arévalo, J. V., Fernandes, P. D., Pérez-Sabino, J. F., & Jorge Ribeiro da Silva, A. (2019). Chemical composition and evaluation of antinociceptive activity of the essential oil of Stevia serrata Cav. from Guatemala. Natural product research, 33(4), 577-579. https://doi.org/10.1080/14786419.2017.1399376
Russo, A., Bruno, M., Avola, R., Cardile, V., & Rigano, D. (2020). Chamazulene-Rich Artemisia arborescens Essential Oils Affect the Cell Growth of Human Melanoma Cells. Plants, 9(8), 1000. https://doi.org/10.3390/plants9081000
Liao, P. C., Lai, M. H., Hsu, K. P., Kuo, Y. H., Chen, J., Tsai, M. C., Li, C. X., Yin, X. J., Jeyashoke, N., & Chao, L. K. (2018). Identification of β-Sitosterol as in Vitro Anti-Inflammatory Constituent in Moringa oleifera. Journal of agricultural and food chemistry, 66(41), 10748-10759. https://doi.org/10.1021/acs.jafc.8b04555
Paniagua-Pérez, R., Flores-Mondragón, G., Reyes-Legorreta, C., Herrera-López, B., Cervantes-Hernández, I., Madrigal-Santillán, O., Morales-González, J. A., Álvarez-González, I., & Madrigal-Bujaidar, E. (2016). Evaluation of the anti-inflammatory capacity of beta-sitosterol in rodent assays. African journal of traditional, complementary, and alternative medicines : AJTCAM, 14(1), 123-130. https://doi.org/10.21010/ajtcam.v14i1.13
Kurano, M., Hasegawa, K., Kunimi, M., Hara, M., Yatomi, Y., Teramoto, T., & Tsukamoto, K. (2018). Sitosterol prevents obesity-related chronic inflammation. Biochimica et biophysica acta. Molecular and cell biology of lipids, 1863(2), 191-198. https://doi.org/10.1016/j.bbalip.2017.12.004
Babu, S., & Jayaraman, S. (2020). An update on β-sitosterol: A potential herbal nutraceutical for diabetic management. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 131, 110702. https://doi.org/10.1016/j.biopha.2020.110702
Shaaban, M. T., Ghaly, M. F., & Fahmi, S. M. (2021). Antibacterial activities of hexadecanoic acid methyl ester and green-synthesized silver nanoparticles against multidrug-resistant bacteria. Journal of basic microbiology, 61(6), 557-568. https://doi.org/10.1002/jobm.202100061
Budiadji, A. F., Mapanawang, A. L., Sedeng, D., Muh, N., Tualeka, A., Fambrene, B. T., Ismail, Latuconcina, K. R., Djafar, Y., & Daud, A. (2016). Identification of hexadecanoic acid compound which in golobe extract (Hornstedtiazingiberaceae). International Journal of Health Medicine and Current Research - Ijhmcr, 1(1), 48-52.
Lee, W., Woo, E. R., & Lee, D. G. (2016). Phytol has antibacterial property by inducing oxidative stress response in Pseudomonas aeruginosa. Free radical research, 50(12), 1309-1318. https://doi.org/10.1080/10715762.2016.1241395
Islam, M. T., Ali, E. S., Uddin, S. J., Shaw, S., Islam, M. A., Ahmed, M. I., Chandra Shill, M., Karmakar, U. K., Yarla, N. S., Khan, I. N., Billah, M. M., Pieczynska, M. D., Zengin, G., Malainer, C., Nicoletti, F., Gulei, D., Berindan-Neagoe, I., Apostolov, A., Banach, M., Yeung, A., … Atanasov, A. G. (2018). Phytol: A review of biomedical activities. Food and chemical toxicology, 121, 82-94. https://doi.org/10.1016/j.fct.2018.08.032
How to Cite
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access)