Study and evaluation antioxidant activity of dietary supplements with green tea extract

Authors

DOI:

https://doi.org/10.14739/2409-2932.2021.2.233306

Keywords:

dietary supplements, green tea, antioxidant activity, potentiometry

Abstract

The aim of the work is to determine and evaluate the antioxidant activity of dietary supplements with green tea extract, as well as to introduce conditional terms of the level of AOA.

Materials and methods. The object of the study was dietary supplements of different manufactures: “Green Tea Extract” of Natural Sources, USA (DS1), “Extract of green tea” of Elit-Pharm, Ukraine (DS2) and “Green tea” of Pharmakom, Ukraine (DS3). Potentiometric measurements were conducted by pH meter Hanna 2550 (Germany) with a combined platinum electrode EZDO 5010. Epigallocatechin-3-O-gallate ˃98.0 % (Sigma Aldrich), K3[Fe(CN)6], K4[Fe(CN)6], NaHPO4, KH2PO4 were analytical grade.

Results. It was established that a value of the antioxidant activity of DS1 was 36.51 mmol/tab, DS2 – 29.78 mmol/tab, and DS3 – 16.67 mmol/tab. DS1 had the highest value of the antioxidant activity, which correlated with the content of catechins (r2 = 0.9314). According to the proposed conditional terms of antioxidant activity dietary supplements, DS1 and DS2 possessed a low level of antioxidant activity, whereas DS3 corresponded to very low level of antioxidant activity.

Conclusions. It was found that the studied dietary supplements with green tea extract have antioxidant activity, which correlates with the content of catechins.

Author Biographies

O. Yu. Maslov, National University of Pharmacy, Kharkiv, Ukraine

Assistant of the Department of Analytical Chemistry and Analytical Toxicology

S. V. Kolisnyk, National University of Pharmacy, Kharkiv, Ukraine

PhD, DSc, Professor, Head of the Department of Analytical Chemistry and Analytical Toxicology

M. A. Komisarenko, National University of Pharmacy, Kharkiv, Ukraine

Candidate of Pharmaceutical Sciences, Assistant of the Department of Pharmacognosy

A. A. Altukhov, National University of Pharmacy, Kharkiv, Ukraine

Candidate of Pharmaceutical Sciences, Associate Professor of the Department of Analytical Chemistry and Analytical Toxicology

K. V. Dynnyk, National University of Pharmacy, Kharkiv, Ukraine

Candidate of Pharmaceutical Sciences, Associate Professor of the Department of Analytical Chemistry and Analytical Toxicology

V. I. Stepanenko, National University of Pharmacy, Kharkiv, Ukraine

Candidate of Pharmaceutical Sciences, Associate Professor of the Department of Analytical Chemistry and Analytical Toxicology

References

Vyatkin, A. V., Pastushkova, E. V., & Feofilaktova, O. V. (2018). Obzor metodov opredeleniya obshchei antioksidantnoi aktivnosti [The review of methods for antioxidant activity determination]. Sovremennaa nauka i innovacii, (1), 58-66. [in Russian].

Costa, A. S. G., Nunes, M. A., Almeida, I. M. C., Carvalho, M. R., Barroso, M. F., Alves, R. C., & Oliveira, M. B. P. P. (2012). Teas, dietary supplements and fruit juices: A comparative study regarding antioxidant activity and bioactive compounds. LWT - Food Science and Technology, 49(2), 324-328. https://doi.org/10.1016/j.lwt.2012.02.030

Houston, N., & Kimball, A. B. (2014). Green Tea Extract. In P. K. Farris (Ed.), Cosmeceuticals and Cosmetic Practice (Ch. 12, pp. 122-132). John Wiley & Sons. https://doi.org/10.1002/9781118384824.ch12

Katekhiny chainogo rasteniya: struktura, aktivnost', primenenie [Catechins of tea: structure, activity, application]. Biotekhnolohiia, 1(3), 25-36. [in Russian].

Singh, B. N., Shankar, S., & Srivastava, R. K. (2011). Green tea catechin, epigallocatechin-3-gallate (EGCG): mechanisms, perspectives and clinical applications. Biochemical pharmacology, 82(12), 1807-1821. https://doi.org/10.1016/j.bcp.2011.07.093

Chu, C., Deng, J., Man, Y., & Qu, Y. (2017). Green Tea Extracts Epigallocatechin-3-gallate for Different Treatments. BioMed research international, 2017, 5615647. https://doi.org/10.1155/2017/5615647

Xu, Z., & Howard, L. R. (2012). Analysis of antioxidant-rich phytochemicals. John Wiley & Sons.

Maslov, O. Y., Kolisnyk, S. V., Hrechana, O. V., & Serbin, A. H. (2021). Study of the qualitative composition and quantitative content of some groups of BAS in dietary supplements with green tea leaf extract. Zaporozhye Medical Journal, 23(1), 132-137. https://doi.org/10.14739/2310-1210.2021.1.224932

Pisoschi, A. M., Cimpeanu, C., & Predoi, G. (2015). Electrochemical methods for total antioxidant capacity and its main contributors determination: A review. Open Chemistry, 13(1), 824-856. https://doi.org/10.1515/chem-2015-0099

Xu, Y. Q., Gao, Y., & Granato, D. (2021). Effects of epigallocatechin gallate, epigallocatechin and epicatechin gallate on the chemical and cell-based antioxidant activity, sensory properties, and cytotoxicity of a catechin-free model beverage. Food chemistry, 339, 128060. https://doi.org/10.1016/j.foodchem.2020.128060

Boulmokh, Y., Belguidoum, K., Meddour, F., & Amira-Guebailia, H. (2021). Investigation of antioxidant activity of epigallocatechin gallate and epicatechin as compared to resveratrol and ascorbic acid: experimental and theoretical insights. Structural Chemistry. https://doi.org/10.1007/s11224-021-01763-5

Tay, P. Y., Tan, C. P., Abas, F., Yim, H. S., & Ho, C. W. (2014). Assessment of extraction parameters on antioxidant capacity, polyphenol content, epigallocatechin gallate (EGCG), epicatechin gallate (ECG) and iriflophenone 3-C-β-glucoside of agarwood (Aquilaria crassna) young leaves. Molecules, 19(8), 12304-12319. https://doi.org/10.3390/molecules190812304

Downloads

Published

2021-06-01

How to Cite

1.
Maslov OY, Kolisnyk SV, Komisarenko MA, Altukhov AA, Dynnyk KV, Stepanenko VI. Study and evaluation antioxidant activity of dietary supplements with green tea extract. Current issues in pharmacy and medicine: science and practice [Internet]. 2021Jun.1 [cited 2024Nov.23];14(2):215-9. Available from: http://pharmed.zsmu.edu.ua/article/view/233306

Issue

Section

Original research