Morphological rearrangement of the metabolic link of the microcirculatory bed of guinea pigs lungs after sensitization with ovalbumin

Authors

DOI:

https://doi.org/10.14739/2409-2932.2021.1.226851

Keywords:

capillary, microcirculation, experimental allergic inflammation, lung, ovalbumin, guinea pig

Abstract

The reaction of the lung microvessels is an urgent issue of morphology and medicine in general, as well as one of the insufficiently investigated points in the study of morphological changes in chronic allergic diseases of the respiratory system.

The aim is to study the morphological changes in the vessels of the metabolic link of the microcirculation in the lungs of guinea pigs sensitized with ovalbumin.

Materials and methods. We have studied the lungs of 48 guinea pigs, using histological and morphometric methods, under conditions of experimental ovalbumin-induced allergic inflammation, simulated by three-time subcutaneous sensitization and subsequent 8-day intranasal inhalation of ovalbumin. To estimate the structural and functional state of capillaries, the inner diameter of the vessels was determined.

Results. A general regularity in the reactivity of the hemomicrocirculatory bed of guinea pig lungs in experimental ovalbumin-induced allergic inflammation was established, which consists in a significant structural and functional restructuring of the exchange vessels of the microcirculatory bed. Dysfunction of the capillaries endothelium is manifested by a change of vasodilatation to vasospasm, as evidenced by morphometric changes in the diameter of the capillaries lumen in the experimental groups, and an increase in the permeability of the capillaries wall, which is confirmed by edema and disorganization of the connective tissue component.

Conclusions. Allergic inflammation leads to structural and functional reorganization of the metabolic link of the hemomicrocirculatory bed of guinea pig lungs, which has a multidirectional staging character depending on the duration of the experiment and is a manifestation of a violation of the compensatory-adaptive processes of hemomicrocirculation. The most pronounced changes in the form of a decrease in the diameter of the lumen of the blood capillaries of the lungs of guinea pigs by 23 % compared to the control are observed during the late period of the development of the allergic inflammatory process.

References

Hrebniak, M. P., & Fedorchenko, R. A. (2019). Influence of industrial atmospheric pollution on the development of pathology of respiratory organs. Pathologia, 16(1), 81-86. https://doi.org/10.14739/2310-1237.2019.1.166314

Lu, S., Li, H., Gao, R., Gao, X., Xu, F., Wang, Q., Lu, G., Xia, D., & Zhou, J. (2015). IL-17A, But Not IL-17F, Is indispensable for airway vascular remodeling induced by exaggerated Th17 cell responses in prolonged ovalbumin-challenged mice. The Journal of Immunology, 194(8), 3557-3566. https://doi.org/10.4049/jimmunol.1400829

Hnatjuk, M. S., & Tatarchuk, L. V. (2018). Morphometric analysis remodeling vessels hemomicrocirculatory bed of jejunum at resections of liver. Reports of Morphology, 24(1), 16-20. https://doi.org/10.31393/morphology-journal-2018-24(1)-03

Reichard, A., & Asosingh, K. (2019). Endothelial Cells in Asthma. In C. Pereira (Ed.), Asthma - Biological Evidences. IntechOpen. https://www.intechopen.com/books/asthma-biological-evidences/endothelial-cells-in-asthma

Pronina, O. M., Koptev, M. M., Bilash, S. M., & Yeroshenko, G. A. (2018). Response of hemomicrocirculatory bed of internal organs on various external factors exposure based on the morphological research data. World of Medicine and Biology, (1), 153-157.

Herasymiuk, I. E., & Vatsyk, M. O. (2019). Features of remodeling of blood vessels of rat lungs in applying different methods of fluid resuscitation after general dehydration. Bulletin of Problems Biology and Medicine, (1, Part 2), 272-276. https://doi.org/10.29254/2077-4214-2019-1-2-149-272-276

Cai, Z., Liu, J., Bian, H., & Cai, J. (2019). Albiflorin alleviates ovalbumin (OVA)-induced pulmonary inflammation in asthmatic mice. American journal of translational research, 11(12), 7300-7309.

Zemmouri, H., Sekiou, O., Ammar, S., El Feki, A., Bouaziz, M., Messarah, M., & Boumendjel, A. (2017). Urtica dioica attenuates ovalbumin-induced inflammation and lipid peroxidation of lung tissues in rat asthma model. Pharmaceutical Biology, 55(1), 1561-1568. https://doi.org/10.1080/13880209.2017.1310905

Antwi, A. O., Obiri, D. D., & Osafo, N. (2017). Stigmasterol modulates allergic airway inflammation in guinea pig model of ovalbumin-induced asthma. Mediators of Inflammation, 2017, 2953930. https://doi.org/10.1155/2017/2953930

Lambrecht, B. N., & Hammad, H. (2014). The immunology of asthma. Nature Immunology, 16(1), 45-56. https://doi.org/10.1038/ni.3049

Ha, E. H., Choi, J.-P., Kwon, H.-S., Park, H. J., Lah, S. J., Moon, K.-A., Lee, S.-H., Kim, I., & Cho, Y. S. (2019). Endothelial Sox17 promotes allergic airway inflammation. Journal of Allergy and Clinical Immunology, 144(2), 561-573. https://doi.org/10.1016/j.jaci.2019.02.034

Popko, S. S., Yevtushenko, V. M., & Syrtsov, V. K. (2020). Influence of pulmonary neuroendocrine cells on lung homeostasis. Zaporozhye Medical Journal, 22(4), 568-575. https://doi.org/10.14739/2310-1210.2020.4.208411

Downloads

Published

2021-03-18

How to Cite

1.
Popko SS. Morphological rearrangement of the metabolic link of the microcirculatory bed of guinea pigs lungs after sensitization with ovalbumin. Current issues in pharmacy and medicine: science and practice [Internet]. 2021Mar.18 [cited 2024Jul.21];14(1):79-83. Available from: http://pharmed.zsmu.edu.ua/article/view/226851

Issue

Section

Original research