A study of acute toxicity of dry extracts of the earth almond (chufa) tubers and herb
Keywords:Cyperus esculentus L. (chufa) herb, plant tubers, extract, acute toxicity
The aim of the work – to study the acute toxicity of dry extracts obtained from herb and tubers of the earth almond.
Materials and methods. The objects of research were dry extracts obtained from herb and tubers of the Cyperus esculentus L. (chufa). Acute toxicity of dry extracts, obtained from the earth almond herb and tubers, was studied with V. B. Prozorovskyi’s method on 42 white nonlinear mice of both sexes weighing 21–25 g, divided into groups (each comprising 3 males and 3 females). The studied extracts were administered once intraperitoneally in doses of 1000 mg/kg, 3000 mg/kg and 5000 mg/kg. The animals were observed for 14 days.
Results. Single intraperitoneal administration of dry extracts obtained from herb and tubers of the earth almond at doses of 1000 mg/kg, 3000 mg/kg and 5000 mg/kg did not affect the dynamics of mice’s body weight in comparison with the control group. Experimental and control animals gained weight in accordance with physiological norms. External examination of the animals showed no signs of pathological changes in their condition: the hair and skin were clean, the subcutaneous layer of adipose tissue was moderate, signs of skin damage and inflammatory lesions were not observed on the mucous membranes. Besides, visual estimation of the internal organs condition showed no signs of pathological changes.
Conclusions. According to the results of acute toxicity determination based on the classification by K. K. Sydorov, dry extracts obtained from herb and tubers of the earth almond, can be attributed to class VI toxicity – almost harmless substances, LD50 >5000 mg/kg.
Mykolaichuk, V. H. (2007). Etapy ta perspektyvy introduktsii Cyperus esculentus L. [Stages and prospects of introduction of the Cyperus esculentus L.]. Introduktsiia roslyn, (4), 38-43. [in Ukrainian].
Grigorovskaya M. V. (1999). Skrytnaya chufa dorogogo stoit [The secretive chufa is worth a lot]. Ogorodnik, (5), 4-5. [in Russian].
Hryhoriuk, I., Rakhmetov, D., & Mashkovska, S. (2003). Chufa. Naukovyi svit, (7), 14-15. [in Ukrainian].
Rashmi, R. (1993). Pharmacognostic studies of Cyperus esculentus tuber. Journal of Plant Anatomy and Morphology (Jodhpur), (6), 56-62.
Mykolaichuk, V. H., Verhun, O. M., & Rakhmetov, D. B. (2011). Dynamika fotosyntetychnykh pihmentiv zalezhno vid rostu i rozvytku roslyn Cyperus esculentus L. pry introduktsii v Pravoberezhnomu Lisostepu Ukrainy [The dynamic of photosynthetic pigments Cyperus esculentus L., introducted in right-bank forest-steppe zone of Ukraine]. Problemy ekolohii ta okhorony pryrody tekhnohennoho rehionu, (1), 242-249. [in Ukrainian].
El-Shebini, S. M., Moaty, M. I. A., Tapoza, S. T., & Hanna L. M. (2011). Short term effect of Cyperus esculentus supplement on body weight, insulin sensitivity and serum lipoproteins in Egyptian obese patients. International Journal of Academic Research, 3(3), 539-544.
Pascual, B., Maroto, J. V., LóPez-Galarza, S., Sanbautista, A., & Alagarda, J. (2000). Chufa (Cyperus esculentus L. var. sativus boeck.): An unconventional crop. studies related to applications and cultivation. Economic Botany, 54, 439-448. https://doi.org/10.1007/BF02866543
Marchyshyn, S. M., Slobodianiuk, L. V., Ivasiuk, I. M., Mishchenko, L. T., Mashkovska, S. P., Demydiak, O. L., Hudz, N. A., & Ambok, Kh. Yu. (2020). Fenolni spoluky likarskykh roslyn, introdukovanykh v Ukraini [Phenolic compounds of medicinal plants introduced in Ukraine]. Cuchasni napriamky udoskonalennia farmatsevtychnoho zabezpechennia naselennia: vid rozrobky do vykorystannia likarskykh zasobiv pryrodnoho i syntetychnoho pokhodzhennia. Proceedings of the International Scientific and Practical Internet-Conference (pp. 169-171). Ivano-Frankivsk: IFNMU. [in Ukrainian].
Marchyshyn, S. M., Slobodianiuk, L. V., & Ivasiuk, I. M. (2019). Doslidzhennia fenolnykh spoluk u travi i bulbakh smykavtsiu yistivnoho (chufy) (Cyperus esculentus L.) [Study of phenolic compounds in the herb and bulbs of the chufa (Cyperus Esculentus L.) using the HPLC method]. Fitoterapiia. Chasopys, (1), 89-92. [in Ukrainian]. https://doi.org/10.33617/2522-9680-2019-1-89
Marchyshyn, S. M., Budniak, L. I., & Ivasiuk, I. M. (2020). Doslidzhennia dubylnykh rechovyn u travi ta bulbakh smykavtsia yistivnoho (chufy) (Cyperus esculentus L.) metodom VERKh [Investigation of tannins in herb and tubers of the yellow nutgrass (Cyperus esculentus L.) (chufa) by HPLC method]. Current issues in pharmacy and medicine: science and practice, 13(2), 225-229. [in Ukrainian]. https://doi.org/10.14739/2409-2932.2020.2.207119
Marchyshyn, S. M., & Ivasiuk, I. M. (2019, September 19-20). Doslidzhennia aminokyslotnoho i vuhlevodnoho skladu travy smykavtsia yistivnoho (Cyperus esculentus L.) [Study of the amino acid and carbohydrate composition of the herb Cyperus esculentus L.]. Modern pharmacy: history, realities and prospects of development. Proceedings of the scientific-practical conference (Vol. 1, pp. 236-237). Kharkiv: NUPh. [in Ukrainian].
Prozorovskii, V. B. (1992). Prakticheskoe posobie po uskorennomu opredeleniyu srednikh effektivnykh doz i kontsentratsii biologicheski aktivnykh veshchestv [A practical guide to the accelerated determination of the average effective doses and concentration of biologically active substances]. SPb. [in Russian].
Mironov, A. N. (Ed.). (2012). Rukovodstvo po provedeniyu doklinicheskikh issledovanii lekarstvennykh sredstv. Ch. 1 [Guidelines for Conducting Preclinical Trials of Medicines (part 1)]. Moscow: Grif and K. [in Russian].
Stefanov, O. V. (Ed.). (2001). Doklinichni doslidzhennia likarskyh zasobiv [Preclinical studies of medicines]. Kyiv: Avitzena. [in Ukrainian].
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