Identification of anise (Anisum vulgare Gaertn.) herbs by morphological and anatomical characteristics




Anisum vulgare, herbs, plant structures


The fruits of anise contain different classes of biologically active compounds, due to which they have antidepressant, antiperoxidant, antidiabetic effect; extracts of this plant have an antibacterial effect. Professional literature lacks information on the chemical composition of anise herbs and prospects for their use in medicine.

The aim of the work is to study of the morphological and anatomical structure of the aboveground organs of anise and to establish of macro- and microscopic diagnostic characters necessary for the identification of the plant raw materials.

Materials and methods. Anise herbs consisting of stems, leaves flowers, and unripe fruits were used for the study. Fresh and dried raw materials were macroscopically analyzed with the naked eye and using a magnifying glass (×10). Studies of transverse and longitudinal sections, epidermis and surface preparations were performed using conventional techniques: with microscopes MBS 9, MS 10 (glasses ×5, ×10, ×15, lenses ×10, ×40), Micromed XS-4130 (eyepiece WF15X, lenses ×40/0.65, ×10/0.25) with a photomicrographic attachment. The micrographs were taken with Samsung PL50 camera.

Results. As a result of the macro- and microscopic analysis, diagnostic properties of anise herbs were established.

Conclusions. It is for the first time that macro- and microscopic characteristics of anise herbs were studied, which confirms the identity of raw materials. The obtained results will be used for the development of domestic normative documentation on anise herbs raw materials.


Sayed-Ahmad, B., Talou, T., Saad, Z., Hijazi, A., & Merah, O. (2017). The Apiaceae: Ethnomedicinal family as source for industrial uses. Industrial Crops and Products, 109, 661-671.

Zengin, G., Mahomoodally, M. F., Paksoy, M. Y., Picot-Allain, C., Glamocilja, J., Sokovic, M., Diuzheva, A., Jekő, J., Cziáky, Z., Rodrigues, M. J., Sinan, K. I., & Custodio, L. (2019). Phytochemical characterization and bioactivities of five Apiaceae species: Natural sources for novel ingredients. Industrial Crops and Products, 135, 107-121.

Terninko, I. I., Kyslychenko, V. S., & Khvorost, O. P. (2011). Porivnialne vyvchennia anatomichnykh diahnostychnykh oznak travy fenkheliu zvychainoho ta kropu zapashnoho [The comparative study of anatomic diagnostic signs of grass of fennel and dill]. Ukrainskyi medychnyi almanakh, 14(6), 202-205. [in Ukrainian].

Marchyshyn, S. M., Sira, L. M., & Chelin, N. V. (2011). Analiz aminokyslotnoho skladu lystkiv, plodiv ta korenevyshch i koreniv liubystku likarskoho (Levisticum officinale Koch.) [Analysis of the amino acid composition of leaves, fruits and rhizomes and roots of lovage (Levisticum officinale Koch.)]. Aktualni problemy profilaktychnoi medytsyny (pp. 120-124). Lviv. [in Ukrainian].

Saibi, S., Belhadj, M., & Benyoussef, E. H. (2012). Essential oil composition of Pimpinella anisum from Algeria. Analytical Chemistry Letters, 2(6), 401-404.

State Enterprise Ukrainian Scientific Pharmacopoeial Center of Medicines Quality (2014). Derzhavna Farmakopeya Ukrayiny [The State Pharmacopoeia of Ukraine] (Vol. 3, 2nd ed.). Kharkiv: State Enterprise Ukrainian Scientific Pharmacopoeial Center of Medicines Quality. [in Ukrainian].

Akbar S. (2020) Pimpinella anisum L. (Apiaceae/Umbelliferae). In Handbook of 200 Medicinal Plants. Springer, Cham.

Iannarelli, R., Caprioli, G., Sut, S., Dall’Acqua, S., Fiorini, D., Vittori, S., & Maggi, F. (2017). Valorizing overlooked local crops in the era of globalization: the case of aniseed (Pimpinella anisum L.) from Castignano (central Italy). Industrial Crops and Products, 104, 99-110.

Tavallali, V., Rahmati, S., & Bahmanzadegan, A. (2017). Antioxidant activity, polyphenolic contents and essential oil composition of Pimpinella anisum L. as affected by zinc fertilizer. Journal of the science of food and agriculture, 97(14), 4883-4889.

Özgüven, M. (2012). Aniseed. In Handbook of Herbs and Spices: Second Edition (Vol. 2, pp. 138-150). Elsevier Inc.

Shahamat, Z., Abbasi-Maleki, S., & Mohammadi Motamed, S. (2016). Evaluation of antidepressant-like effects of aqueous and ethanolic extracts of Pimpinella anisum fruit in mice. Avicenna journal of phytomedicine, 6(3), 322-328.

Shobha, R. I., Rajeshwari, C. U., & Andallu, B. (2013). Antiperoxidative and anti-diabetic activities of aniseeds (Pimpinella anisum L.) and identification of bioactive compounds. American Journal of Phytomedicine and Clinical Therapeutics, 1(5), 516-527.

Islam, Z. M., Khan, K., Rakhshanda, S., Mahdi, R., & Chowdhury, I. M. (2016). Antibacterial and phytochemical screening of Pimpinella anisum through optimized extraction procedure. Asian Journal of Science and Technology, 7(11), 3912-3918



How to Cite

Kolisnyk SV, Gontova TM, Umarov UA, Hordiei KR. Identification of anise (Anisum vulgare Gaertn.) herbs by morphological and anatomical characteristics. Current issues in pharmacy and medicine: science and practice [Internet]. 2021Mar.18 [cited 2024Jun.21];14(1):39-44. Available from:



Original research