Chromato-mass spectroscopy of tinctures of Valeriana officinalis aerial part




Valeriana officinalis, Valerian tincture, gas chromatography-mass spectroscopy, component composition, quantitative content


Underground Valerian roots (Vаlеrіаnае radix) are officinal raw materials. More than 1000 compounds were detected in Valeriana officinalis L. s.l., of which no less than 500 natural substances of organic and inorganic origin were identified, among them – carbohydrates, amino acids, lipids, vitamins, enzymes, phenolic compounds, essential oil, iridoids, alkaloids, steroids. The aerial part of Valerian is a source of various flavonoids with pronounced antioxidant properties and specific pharmacotherapeutic activity.

The aim of the research is to determine, by means of gas chromatography, component composition of Valerian tinctures, made of the aerial part of V. tuberosa L., V. stolonifera Czern (Kantserivska balka, Zaporizhzhia region, Ukraine) and V. officinalis L. (Łódź, Bydgoszcz, Poland).

Materials and methods. Tincture samples of the aerial part of wild species V. tuberosa L., V. stolonifera Czern (Kantserivska balka, Zaporizhzhia region), and V. officinalis L. (the experimental site of ZSMU; Łódź and Bydgoszcz in Poland) were selected for experimental studies according to the traditional production recipe for making tinctures (1 : 5). Component composition of the tinctures was studied using a 7890B gas chromatograph with a 5977B mass spectrometer detector.

Results. By means of chromato-mass spectrometry, component composition of the Valerian tinctures has been identified, namely: in the tincture prepared from the aerial part of V. officinalis (Łódź, Poland) 54 components have been identified, V. tuberosa – 50; V. stolonifera – 46 and V. officinalis (Bydgoszcz, Poland) – 30. The chromatogram analysis of four Valerian tinctures helped to identify 118 components, 5 of which were present in all studied tinctures; 12 components were present in three tinctures; 22 components have been found in two tinctures. It should be noted that 10 components have been found in the tinctures in large quantities, their content ranging from 10.91 % to 30.02 %.

Conclusions. Analyzing the obtained data of the GC, it can be seen that Valerian tinctures differ both in quantitative and qualitative composition. From the aerial part of Valeriana officinalis, prepared in Łódź and Bydgoszcz in Poland, 17 components coincide. In the tinctures, prepared from herbs collected in Ukraine, 10 components coincide. The highest content of Butanoic acid, 3-methyl – from 4.01 % to 16.78 %; 9,12,15-Octadecatrienoic acid, ethyl ester, (Z,Z,Z)- – from 0.47 % to 7.09 %; Dihydroxyacetone – from 0.37 % to 5.69 % and Quinic acid – from 0.30 % to 4.66 % in all four tinctures by peak areas and retention time should be marked. In three tinctures Cyclododecane, 1,5,9-tris(acetoxy)- has been identified in amount of 30.56 % to 14.56 %. The obtained results confirm the expediency of using the aerial part of Valeriana officinalis as raw material with a large commodity yield and various contents of biologically active substances for further pharmacognostic researches for the purpose of creation of new medicines and phytopreparations


Korniievskyi, Yu. I., Korniievska, V. H., Panchenko, S. V., & Bohuslavska, N. Yu. (2014). Valeriana likarska [Valerian Medicines]. Zaporizhzhia: ZSMU. [in Ukrainian].

Kryvenko, V. I., Korniievskyi, Yu. I., Kolesnyk ,M. Yu, Korniievska, V. H., & Demchenko, A. V. (2018). Fitoterapiia insomnii [Phytotherapy of insomnia] (2nd.). Zaporizhzhia : ZSMU. [in Ukrainian].

Upton, R., & Petrone, C. (Eds.). (1999). Valerian Root. American Herbal Pharmacopoeia and Therapeutic Compendium. Santa Cruz.

Bos, R., Hendriks, H., Pras, N., Stojanova, A. S., & Georgiev, E. V. (2000). Essential oil composition of Valeriana officinalis ssp. collina cultivated in Bulgaria. Journal of Essential Oil Research, 12(3), 313-316.

Council of Europe. (2005). European Pharmacopoeia (Vol. 2, 5th ed.), (pp. 2667-2668). Strasbourg.

Korniievska, V. H., Sur, S. V., Korniievskyi, Yu. I., & Fursa, M. S. (2000). Sklad efirnoi olii – diahnostychna oznaka syrovyny valeriany [Composition of essential oil – diagnostic sign of raw materials of valerian]. Nauchnye napravleniya v sozdanii lekarstvennykh sredstv v farmatsevticheskom sektore Ukrainy. Materials of Scientific Conference (pp. 154-156). Kharkiv. [in Ukrainian].

Korniievskyi, Yu. I., Kucherenko, L. I., Korniievska, V. H., Skoryna, D. Yu., Khromylova, O. V., & Bohuslavska, N. Yu. (2020). Likarski roslyny na aptechnii polytsi [Medicinal plants on the pharmacy shelf]. Zaporizhzhia: ZSMU. [in Ukrainian].

State Enterprise Ukrainian Scientific Pharmacopoeial Center of Medicines Quality. (2008, February 1). Derzhavna Farmakopeia Ukrainy. Dopovnennia 2 [The State Pharmacopoeia of Ukraine] (1st ed., Suppl 1). Kharkiv: Naukovo-ekspertnyi farmakopeinyi tsentr. [in Ukrainian].

State Enterprise Ukrainian Scientific Pharmacopoeial Center of Medicines Quality. (2001). Derzhavna Farmakopeia Ukrainy [The State Pharmacopoeia of Ukraine] (1st ed.). Kharkiv: Naukovo-ekspertnyi farmakopeinyi tsentr. [in Ukrainian].

(2017). Tekhnolohichnyi rehlament TP 64-01973472-069-17 na vyrobnytstvo likarskoho zasobu "Valeriany nastoika, nastoika po 25 ml u flakonakh v pachtsi abo bez pachky" [Valerian tincture, tincture of 25 ml in vials in a pack or without a pack]. Zaporizhzhia: Viola. [in Ukrainian].

Korniievskyi, Yu. I. Odyntsova, V. M. Korniievska, V. H. Kandybei, N. V. & Bohuslavska, N. Yu. (2019). Tekhnolohiia vyrobnytstva ta khromato-mas-spektroskopiia nastoiok valeriany likarskoi [Production technology and chromato-mass spectroscopy of the valeriana officinalis tinctures]. Current issues in pharmacy and medicine: science and practice, 12(2), 172-180. [in Ukrainian].



How to Cite

Odyntsova VM, Kokitko VI, Korniievska VH, Korniievskyi YI, Karpun YO. Chromato-mass spectroscopy of tinctures of Valeriana officinalis aerial part. Current issues in pharmacy and medicine: science and practice [Internet]. 2021Mar.18 [cited 2024Feb.26];14(1):29-38. Available from:



Original research