Optimization of glycine and thiotriazoline compound analysis by high-performance liquid chromatography
Keywords:glycine, thiotriazoline, tablets, amino acids, HPLC
Today, Ukraine is ranked first in Europe in terms of cerebrovascular morbidity and mortality from stroke. Therefore, the creation of new drugs for the treatment of these pathologies is an urgent task of modern pharmacy. For that reason, the creation of a new combination drug based on glycine – a neurotransmitter amino acid and thiothiazolin – an antioxidant is appropriate and relevant. For the new combination drug, a rational dosage form in the form of tablets was proposed. New standardization methods should be developed for the combination of tablets. Nowadays, physical-chemical methods of research are widely used for standardization of finished dosage forms, both in pharmacy and factory production. Our attention was drawn to the method of high-performance liquid chromatography, which allows us to simultaneously standardize the active substances in one sample. To develop the HPLC methodology, it is first necessary to select the optimal conditions for the analysis of active substances.
The purpose of work is a selection of optimal conditions for the simultaneous determination of glycine with thiotriazoline in the model mixture by HPLC method.
Materials and methods. In the course of the research, glycine, thiotriazolin were used. The studies were performed using a BISCHOFF HPLC modular system with a Lambda 1010 spectrophotometric detector. The columns used were: Prontosil 120-5-CN, Hypersil ODS-C18-5u. As eluent used: water; 0.05 % aqueous trifluoroacetic acid solution; Bu4NHSO4 3.4 g/l in water; Bu4NHSO4 3.4 g/l, 0.02 M Na2HPO4 in water; Bu4NHSO4 3.4 g/l, 0.05 % trifluoroacetic acid solution in water.
Results. As can be seen from the results obtained, it is expedient to use tetrabutylammonium in the conditions of pair chromatography on the reverse phase, among different eluents and phases used, for the subsequent determination of glycine with thiotriazolin, both in the model mixture and in the combined dosage forms with the simultaneous use of acid buffer – 0.05 % solution of trifluoroacetic acid.
Conclusions. During the research, the optimal conditions for the simultaneous determination of glycine with thiotriazoline in one sample by HPLC were selected. Іt was found that the determination of the active substances should be carried in the conditions of pair chromatography on the reverse phase when using tetrabutylammonium eluent with the simultaneous use of acid buffer – 0.05 % solution of trifluoroacetic acid
Kucherenko, L. І., Khromylova, O. V., & Levykh, A. E. (2016). Primenenie neirotransmitternych aminokislot v sovremennoy meditsine [Usе оf neurotransmitter aminoacids in modern medicine]. Retsept, 19(5), 616-620. [in Russian].
Horchakova, N. A, Belenichev, I. F, & Mazur, I. A. (2007). Mekhanizm antioksidantnoho i protivoishemicheskoho deystviya tiotriazolina [The mechanism of antioxidant and anti-ischemic action of thiotriazolin]. Novosti meditsyny i farmatsii, (2), 11-21. [in Russian].
Mazur, I. A., Voloshin, N. A., Chekman, I. S., Zimenkovskii, B. S., & Stets, V. R. (2005). Tiotriazolin: farmakologicheskie aspekty i klinicheskoe primenenie [Thiotriazoline: pharmacological aspects and clinical usage]. Zaporozhye; Lvov: Nautіlus. [in Russian].
Mazur, I. A., Chekman, I. S., Belenichev, I. F., & Kucherenko, L. I. (2011). Razrabotka lekarstvennykh sredstv na osnove fiksirovannykh kombinatsii s antioksidantami – perspektivnoe napravlenie sovremennoi farmakologii [The development of medicines based on fixed combinations with antioxidants is a promising area of modern pharmacology]. Farmakolohiia ta likarska toksykolo-hiia, (5), 199-200. [in Russian].
Kucherenko, L. I., Khomylova, O. V., Mazur, І. А., Belenichev, I. F., & Horbacheva, S. V. (2017). Ukrainian Patent No. 114270. Kombinovanyi likarskyi zasib dlia pervynnoi neiroprotektsii [Combination drug for primary neuroprotection]. Retrieved from https://base.uipv.org/searchINV/search.php?action=viewdetails&IdClaim=235074
Kucherenko, L., Belenichev, I., Mazur, I., Khromylova, O., & Parniuk, N. (2018). Influence of the fixed combination of glycine with thiotriazoline on energy metabolism parameters in brain in conditions of experimental cerebral ischemia. Ankara Universitesi Eczacilik Fakultesi Dergisi, 42(1), 14-21. https://doi.org/10.1501/Eczfak-0000000598
Kucherenko, L. І., Khromylova, O. V., Moriak, Z. B., & Tkachenko, H. I. (2014). Pidbir optymalnykh umov analizu sumishi izoniazidu z tiotryazolinom metodom vysokoefektyvnoi ridynnoi khromatohrafii [Choice of optimal conditions of Isoniazid and Thiotriazolin artifi cial mixture analysisby high-performance liquid chromatography method]. Zaporozhye medical journal, (2), 118-120. [in Ukrainian].
Kucherenko, L. I., Mazur, I. А., Khromylova, O. V., & Moriak, Z. B. (2018). Optimization of l-arginine and thiotriazoline compound analysis by high-performance liquid chromatography. Zaporozhye medical journal, 20(6), 837-840. https://doi.org/10.14739/2310-1210.2018.6.146760
Sadek, P. (2009). Rastvoriteli dlya VEZhKh [Solvents for HPLC]. Moscow: Binom. Laboratoriya znanii. [in Russian].
Saprykin, L. V. (2006). Praktika i metodicheskie osnovy vysokoeffektivnoi zhidkostnoi khromatografii [Practical and methodological foundations of high-performance liquid chromatography]. Krasnodar. [in Russian].
State Enterprise Ukrainian Scientific Pharmacopoeial Center of Medicines Quality. (2014). Derzhavna Farmakopeia Ukrainy [The State Pharmacopoeia of Ukraine] (2nd ed.). Vol. 1. Kharkiv: State Enterprise Ukrainian Scientific Pharmacopoeial Center of Medicines Quality. [in Ukrainian].
How to Cite
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access)