Investigation of tannins in herb and tubers of the yellow nutgrass (Cyperus esculentus L.) (chufa) by HPLC method
Keywords:cyperus, tannins, plants, tubers, high performance liquid chromatography
The aim of the work ‒ determination of the qualitative composition and quantitative content of the individual components of tannins in the herb and tubers of the yellow nutgrass (chufa) (Cyperus esculentus L.) by the method of HPLC.
Materials and Methods. Herb and tubers of the yellow nutgrass were objects for the research. The raw material was harvested at the experimental sites of the New Cultures Department of M.M. Hryshko National Botanic Garden of the NAS of Ukraine in Kyiv in 2018. The qualitative composition and quantitative content of the components of tannins were determined by high performance liquid chromatography (HPLC).
Results. 7 components of tannins were found out in the herb and tubers of the yellow nutgrass by the HPLC method. Among the individual components of tannins in the herb and tubers of the yellow nutgrass, gallocatechin and epigallocatechin predominate. In the herb of the yellow nutgrass, the lowest content of ellagic acid was set, which was 0.05 %. In the tubers of the yellow nutgrass, among the individual components of tannins, a small amount of gallic, ellagic, acids and catechin was established, their content was 0.1 %.Conclusions. HPLC at first was investigated the qualitative composition and quantitative content of the individual components of tannins in the herb and tubers of the yellow nutgrass. It was established that the studied raw materials contain free gallic and ellagic acids, components of condensed tannins: catechin, gallocatechin, epicatechin, epigallocatechin, epicatechin gallate. Among the individual components of tannins, gallocatechin and epigallocatechin prevailed, the content of which in the herb was 2.19 % and 1.06 %, in tubers – 0.38 % and 0.10 %, respectively. Gallocatechin and epigallocatechin can be recommended to standardize the raw materials of the yellow nutgrass. The results obtained indicate the prospect of further studies of biologically active substances of herb and tubers of the yellow nutgrass
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