Investigation of the qualitative composition and quantitative content of flavonoids in the herbal antidiabetic collections № 3 and № 4 by the method of HPLC

Authors

  • A. O. Savych Ivan Horbachevsky Ternopil National Medical University of the Ministry of Health of Ukraine,
  • S. M. Marchyshyn Ivan Horbachevsky Ternopil National Medical University of the Ministry of Health of Ukraine,
  • L. O. Kravchuk Ivan Horbachevsky Ternopil National Medical University of the Ministry of Health of Ukraine,

DOI:

https://doi.org/10.14739/2409-2932.2020.2.207118

Keywords:

herbal antidiabetic collection, flavonoids, high performance liquid chromatography, diabetes mellitus

Abstract

 

Pharmacotherapy of diabetes mellitus is an extremely important problem of medicine and pharmacy due to its deficient safety and imperfection in maintaining optimal glycemia, frequent changes of which lead to the development of diabetes angiopathies. Therefore, the optimization of existing treatments with herbal collections is appropriate because of their low toxicity and the complex effect of the number of biologically active substances on the pathogenesis of diabetes and its complications.

The aim of the research was to determine the qualitative composition and quantitative content of flavonoids in the herbal antidiabetic collections № 3 and № 4.

Materials and methods. The objects of the study were the herbal antidiabetic collection № 3, which consists of folium Urticae, radix Cichorii, fructus Rosae majalis, rhizoma Erytrigiae repens, radix Taraxaci officinale, and the herbal antidiabetic collection № 4, which consists of radix Arctii lappa, rhizoma Erytrigiae repens, styli cum stigmatis Zeae maydis, flores Helichrysi arenarium, fructus Rosae majalis. The qualitative composition and quantitative content of flavonoids were determined by the method of high-performance chromatography using liquid chromatograph Agilent Technologies 1200.

Results. It was identified 6 flavonoid and it was established their quantitative content, among which the rutin (0.36 %), neohesperidin (0.31 %), luteolin (0.17 %), quercetin (0.05 %), isoquercetrin 0.04 % and naringenin 0.04 % in the herbal antidiabetic collection № 3. It was identified 4 flavonoid and it was established their quantitative content, among which luteolin (0.42 %), rutin (0.29 %), quercetin (0.63 %) and isoquercetrin (0.63 %) in the herbal antidiabetic collection № 4.

Conclusions. For the first time, it was determined the qualitative composition and quantitative content of the main flavonoids in the herbal antidiabetic collections № 3 and № 4 by the method of high-performance liquid chromatography. The results of the study showed that the herbal antidiabetic collection № 3 contains 6 flavonoids with a predominance of rutin and neohesperidin, and the herbal antidiabetic collection № 4 – 4 flavonoids, among which luteolin, rutin, and quercetin predominate. This gives reason to conclude about the antidiabetic activity by the pathogenetic mechanism of influence, which indicates that the feasibility of further study of these collections as promising herbal remedies for the treatment and prevention of diabetes mellitus.

 

References

American Diabetes Association. (2017). Standards of Medical Care in Diabetes – 2017. Diabetes care. Vol. 40. https://doi.org/10.2337/dc17-s003

Harding, J. L., Pavkov, M. E., Magliano, D. J., Shaw, J. E., & Gregg, E. W. (2019). Global trends in diabetes complications: a review of current evidence. Diabetologia, 62(1), 3-16. https://doi.org/10.1007/s00125-018-4711-2

Tronko, M. D., & Chernobrov, A. D. (2005). Epidemiolohiia tsukrovoho diabetu v Ukraini [Epidemiology of diabetes mellitus in Ukraine]. Zdorovia Ukrainy, (18), 15. [in Ukrainian].

Hayes, A. J., Leal, J., Gray, A. M., Holman, R. R., & Clarke, P. M. (2013). UKPDS Outcomes Model 2: a new version of a model to simulate lifetime health outcomes of patients with type 2 diabetes mellitus using data from the 30 year United Kingdom Prospective Diabetes Study: UKPDS 82. Diabetologia, 56(9), 1925-1933. https://doi.org/10.1007/s00125-013-2940-y

Lykashiv, O. Jа. (2018). Vykorystannia biolohichno aktyvnykh rechovyn dlia profilaktyky i rehuliatsii metabolizmu pry tsukrovomu diabeti [Using biologically active substances for the prevention and regulation of metabolism in diabetes]. Naukovi zapysky Ternopilskoho natsionalnoho pedahohichnoho universytetu imeni Volodymyra Hnatiuka. Seriia: Biolohiia, (1), 119-122. [in Ukrainian].

Tsal, O., & Lysiuk, R. (2018). Zastosuvannia likarskykh roslyn pry tsukrovomu diabeti [The use of medicinal plants in diabetes]. Kvity Ukrainy, (5), 25-31. [in Ukrainian].

Man’kovskii B. M. Aktualnye voprosy diabetologii v Ukraine [Actual issues of diabetology in Ukraine]. Zdorovia Ukrainy, (11), 6-7. [in Russian].

Demchuk, M. B., Kolisnyk, Yu. V., & Groshovuy T. A. (2015). Porivnialni doslidzhennia asortymentu likarskykh preparativ dlia likuvannia tsukrovoho diabetu 2 typu v Ukraini, Polshchi ta Nimechchyni [Comparative investigations of medicines assortment for the treatment of diabetes mellitus type 2 in Ukraine, Poland and Germany]. Upravlinnia, ekonomika ta zabezpechennia yakosti v farmatsii, (3), 77-83. [in Ukrainian].

Qnais, E., Raad, D., & Bseiso, Y. (2014). Analgesic and anti-inflammatory ef-fects of an extract and flavonoids from Artemisia Herba-Alba and their mechanisms of action. Neurophysiology, 46(3), 238–246. https://doi.org/10.1007/s11062-014-9435-4

Kawabata, K., Mukai, R., & Ishisaka, A. (2015). Quercetin and related poly-phenols: new insights and implications for their bioactivity and bioavailability. Food & Function, 6(5), 1399-1417. https://doi.org/10.1039/c4fo01178c

Li, Y., Yao, J. Y., Han, C. Y., Yang, J. X., Chaudhry, M. T., Wang, S. N., Liu, H. N., & Yin, Y. L. (2016). Quercetin, Inflammation and Immunity. Nutrients, 8(3). https://doi.org/10.3390/nu8030167

Jeong, S. M., Kang, M. J., Choi, H. N., Kim, J. H., & Kim, J. I. (2012). Quercetin ameliorates hyperglycemia and dyslipidemia and improves antioxidant status in type 2 diabetic db/db mice. Nutrition Research and Practice, 6(3), 201-207. https://doi.org/10.4162/nrp.2012.6.3.201

Kuznetsova, I. (2014). Study of thermodynamics of complex formation of fla-vonoids of stevia (Stevia rebaudiana Bertoni) leaves. Vostochno-Evropeiskii zhurnal peredovykh tekhnologii, 2(12). 47-50. https://doi.org/10.15587/1729-4061.2014.23861

Wu, W. J., Li, R. C., Li, X. L., He, J., Jiang, S. B., Liu, S. W., & Yang, J. (2016). Quercetin as an Antiviral Agent Inhibits Influenza A Virus (IAV) Entry. Virus-es-Basel, 8(1), Article 6. https://doi.org/10.3390/v8010006

Tovstukha, Ye. S. (2010). Zoloti retsepty ukrainskoi narodnoi medytsyny [Golden recipes of Ukrainian folk medicine]. Kyiv: KM. [in Ukrainian].

Pyrzynska, K., & Sentkowska, A. (2019). Chromatographic Analysis of Poly-phenols. In Polyphenols in Plants (pp. 353-364). Academic Press.

Zhu, D. Q., Liu, K., Yi, J. L., Liu, B. L., & Liu, G. L. (2011). Luteolin inhibits in-flammatory response and improves insulin sensitivity in the endothelium. Biochimie, 93(3), 506-512. https://doi.org/10.1016/j.biochi.2010.11.002

Hudzenko, A. V., Tsurkan, O. O., & Kovalchuk, T. V. (2011). Dosli­dzhennia preparativ ta roslynnykh sumishei travy dereviiu zvychainoho (Achillea mille-folium L.) [Investigations of preparations and herbal mixtures of herb of Achillea millefolium (Achillea millefolium L.)]. Current issues in pharmacy and medicine: science and practice, 14(2), 62-64. [in Ukrainian].

How to Cite

1.
Savych AO, Marchyshyn SM, Kravchuk LO. Investigation of the qualitative composition and quantitative content of flavonoids in the herbal antidiabetic collections № 3 and № 4 by the method of HPLC. Current issues in pharmacy and medicine: science and practice [Internet]. 2020Jul.3 [cited 2024Feb.26];13(2). Available from: http://pharmed.zsmu.edu.ua/article/view/207118

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Original research