Study of the effect of phytocomposition based on the drug “Arthritan” on the ultrastructure of cartilage in rats with systemic steroid arthritis

Authors

  • Yu. M. Naboka National University of Pharmacy, Kharkiv, Ukraine,
  • N. P. Zubytska LLC “Zemna’s Green Planet”, Kyiv, Ukraine,
  • I. A. Zupanets National University of Pharmacy, Kharkiv, Ukraine,
  • S. K. Shebeko National University of Pharmacy, Kharkiv, Ukraine,
  • I. A. Otrishko National University of Pharmacy, Kharkiv, Ukraine,

DOI:

https://doi.org/10.14739/2409-2932.2020.1.198176

Keywords:

“Arthritan”, chondroprotective activity, cartilage, arthritis

Abstract

 

Recently, there has been a trend towards wider use of herbal medicines, which is associated with their pharmacodynamic polytropy, a fairly high level of safety and economic affordability.

The aim of the work is the study chondroprotective properties of phytocomposition based on the drug “Artritan” under the conditions of evaluation of the ultrastructure of the cartilage tissue of rats with experimental arthritis.

Materials and methods. The study of chondroprotective properties of the investigated phytocomposition based on the drug “Arthritan” was conducted under the conditions of systemic steroid arthritis modeling in rats. Animals received phytocomposition at a conditional therapeutic dose of 0.1 ml/kg. The effectiveness of the phytocomposition was evaluated by the results of the rats' articular cartilage ultrastructure study.

Results. The results of the cartilage tissue ultrastructure study in rats with experimental arthritis under the influence of phytocomposition indicate that this drug has a positive effect on the course of systemic steroid arthritis in rats. Moreover, in terms of the degree of influence on the biosynthetic processes in chondrocytes and the structure of the cartilage matrix, it is not inferior to the activity of the reference drug “Arthron Flex”. Phytocomposition causes positive changes, which are manifested by increased biosynthetic processes in the cells. First of all, this is evidenced by the increase in the content of membrane structures in the form of long profiles of granular endoplasmic reticulum and Golgi plate complexes and the disappearance of chondrocytes at different stages of cell death in micropreparations.

Conclusions. On the model of systemic steroid arthritis in rats, a positive therapeutic effect of the studied phytocomposition was established along with the well-known chondroprotector “Arthron Flex”, which was confirmed by the improvement of the ultrastructure of the articular tissue.

References

Harna, S. V., Vladymyrova, I. M., Burd, N. B., Heorhiiants, V. A., Kotov, A. H., Prokopenko, T. S., … Bondarieva, L. V. (2016). Suchasna fitoterapiia [Modern phytotherapy]. Kharkiv: Madrid Printing House. [in Ukrainian].

Tumanov, V. A., Pokanevich, V. V., Garnik, T. P., Frolov, V. M., & Peresadin, M. O. (2012). Fitoterapiia: suchasni tendentsii do vykorystannia v likarskii praktytsi ta perspektyvy podalshoho rozvytku (ohliad literatury ta rezultaty vlasnykh doslidzhen) [Phytotherapy: application modern lines in medical practice and perspectives of the further development]. Fitoterapiia, 1, 4-11. [in Ukrainian].

Bone, K., & Mills, S. (2012). Principles and practice of phytotherapy: Modern herbal medicine. Principles and practice of phytotherapy: Second edition (pp. 1-1051) https://doi.org/10.1016/C2009-0-48725-7

Heinrich, M., Barnes, J., Gibbons, S., & Williamson, E. M. (2012). Fundamentals of pharmacognosy and phytotherapy (2nd ed.). London: Churchill Livingstone Elesvier.

Kunle, O. F., Egharevba, H. O., & Ahmadu, P. O. (2012). Standardization of herbal medicines: A review. International Journal of Biodiversity and Conservation, 4(3), 101-112. https://doi.org/10.5897/IJBC11.163

Bachurin G. V. (2015). Fitoterapiya – sostavnaya chast' v kompleksnom lechenii patologii nizhnikh mochevykh putei [Phytotherapy as the part of the complex treatment of lower urinary tract diseases]. Current issues of pharmaceutical and medical science and practice, (2), 84-87. [in Russian]. http://dx.doi.org/10.14739/2409-2932.2015.2.45155

Korzh, N. A., Dedukh, N. V., & Zupanets, I. A. (2007). Osteoartroz: konservativnaya terapiya [Osteoarthrosis: conservative therapy]. Khar'kov: Zolotye stranitsy. [in Russian].

Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes. (2010). Official Journal of the European Union, L276, 33-79.

National Research Council. (2011). Guide for the Care and Use of Laboratory Animals (8th ed.). Washington, DC: The National Academies Press.

Zupanets, I. A., Korzh, N. A., Dedukh, N. V., Kovalenko, V. N., Mal'tsev, V. I., Efimtseva, T. K., … Khvisyuk, A. N. (1999). Metodicheskie rekomendatsii po eksperimental'nomu issledovaniyu i klinicheskomu izucheniyu protivoartroznykh (khondromoduliruyushchikh) lekarstvennykh sredstv [Guidelines for the experimental investigation and clinical study of anti-arthritic (chondromodulative) drugs]. Kiev. [in Russian].

Nosivets, D. S. (2018). Eksperimental'nye modeli patologii khryashchevoi tkani. [Experimental models of cartilage tissue pathology]. Zaporozhye medical journal, 21(4), 554-560. [in Russian]. https://doi.org/10.14739/2310-1210.2019.4.173362

Zupanets, K. O., Shebeko, S. K., & Otrishko, I. A. (2010). Doslidzhennia vplyvu kompozytsii na osnovi kvertsetynu ta pokhidnykh hliukozaminu na protsesy apoptozu khondrotsytiv v umovakh rozvytku eksperymentalnoho osteoartrozu [Research of Influence of Composition Containing Quercetine and the Derivates of Glucosamine on Chondrocyte Apoptosis Processes at the Conditions of Experimen-Tal Osteoarthritis Development]. Liky Ukrainy plius, 3, 47-50. [in Ukrainian].

Kuznetsov, S. L., & Mushkambarov, N. N. (2007). Gistologiya, tsitologiya i embriologiya [Histology, cytology and embryology]. Moscow: Meditsinskoe informatsionoe agentstvo. [in Russian].

Sarkisova, D. S., & Perova, Yu. L. (1996). Mikroskopicheskaya tehnika [Microscopic technology]. Moscow: Meditsina. [in Russian].

Downloads

How to Cite

1.
Naboka YM, Zubytska NP, Zupanets IA, Shebeko SK, Otrishko IA. Study of the effect of phytocomposition based on the drug “Arthritan” on the ultrastructure of cartilage in rats with systemic steroid arthritis. Current issues in pharmacy and medicine: science and practice [Internet]. 2020Mar.10 [cited 2024Apr.18];13(1). Available from: http://pharmed.zsmu.edu.ua/article/view/198176

Issue

Vol. 13 No. 1 (2020)

Section

Original research

License

Authors who publish with this journal agree to the following terms:

    1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.     Лицензия Creative Commons
    2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
    3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access)