Study of the anatomical structure of the herbal raw materials of Achillea micranthoides Klok. et Krytzka

Authors

  • I. F. Duyun Zaporizhzhia State Medical University, Ukraine,
  • O. V. Mazulin Zaporizhzhia State Medical University, Ukraine,
  • T. V. Oproshanska National University of Pharmacy, Kharkiv, Ukraine,

DOI:

https://doi.org/10.14739/2409-2932.2020.1.198137

Keywords:

microscopy, anatomical structure, characteristic diagnostic features, Achillea micranthoides Klok. et Krytzka

Abstract

 

Herbal raw materials and herbal drugs derived from it constitute a complete material if they comply with current regulatory documents. This compliance is determined by pharmacognostic analysis. For this, it is necessary to establish the authenticity and high quality of medicinal plant materials. Authenticity is usually confirmed by macro- and microscopic analysis.Promising for implementation in medical practice is the poorly studied species Achillea micranthoides Klok. et Krytzka.

This species is phylogenetically close to the Achillea millefolium L. The plant has a large raw material base and a long growing season. To identify it, for the first time, studies were carried out on the features of the anatomical structure of the herbal raw material and diagnostic microscopic signs were revealed.

The purpose of the work was to study of the anatomical and microscopic structure of Achillea micranthoides Klok. et Krytzka herbal raw material.

Materials and methods. To study the microscopic structure. We used Achillea micranthoides Klok. et Krytzka species collected in the mass flowering phase. Fresh vegetable raw materials were fixed in a mixture of glycerin, ethanol 96 %, purified water (1: 1: 1).

Results. During microscopic studies, features of the anatomical structure of the epidermis of the leaf, main vein, petiole, stem, inflorescence axis, basket wrapper and corolla were revealed.

Conclusions. The characteristic diagnostic features were established for the raw materials of the yarrow of the hearth: a longitudinally wrinkled cuticle on the lower epidermis of the leaf; multicellular double-row hairs and essential oil glands on the upper and lower epidermis; simple, 4–6 cell hairs, in which the lower cells are compressed and the upper one is very long and creates a cobwebby fringe, on the lower epidermis of the leaf and on the epidermis of the petiole, vein, stems and main axis of the inflorescence; sclerenchyma cover from the side of phloem and xylem in conducting bundles of vein and petiole; lamellar-angular collenchyma in the stem and main axis of the inflorescence; longitudinally wrinkled cuticle on epidermal cells in the head axis of the inflorescence; endoderm and sclerenchyma cover over the phloem of the leading bundles.

 

References

Kurtto, A., Sennikov, A., & Lampinen, R. (Eds.) (2018). Atlas Florae Europaeae: Distribution of Vascular Plants in Europe: Vol. 17. Helsinki: The Committee for Mapping the Flora of Europe & Societas Biologica Fennica Vanamo.

Bączek, K., Kosakowska, O., Przybył, J., Kuźma, P., Ejdys, M., Obiedziński, M., & Węglarz, Z. (2015). Intraspecific variability of yarrow (Achillea millefolium L. s.l) in respect of developmental and chemical traits. Herba Polonica, 61(3), 37-52. http://dx.doi. org/10.1515/hepo-2015-0021

K'osev, P. A. (2011). Lekarstvennye rasteniya: samyi polnyi spravochnik [Medicinal plants: the most comprehensive reference]. Moscow: Eksmo. [in Russian].

Turkmenoglu, F. P., Agar, O. T., Akaydin, G., Hayran, M., & Demirci, B. (2015). Characterization of Volatile Compounds of Eleven Achillea Species from Turkey and Biological Activities of Essential Oil and Methanol Extract of A. hamzaoglui Arabaci & Budak. Molecules, 20(6), 11432-11458. https://doi.org/10.3390/molecules200611432

Chernykh, V. P. Pertsev, I. M. Andrushchenko, S. V. Nezhurina, S. A., & Lytkin D. V. (Eds.). (2016). Farmatsevtychna entsyklopediia [Pharmaceutical encyclopedia] (3rd.). Kyiv: Morion. [in Ukrainian].

Prokudin, Yu. N. (Ed.). (1987). Opredelitel' vysshikh rastenii Ukrainy [The Key to Higher Plants of Ukraine]. Kyiv: Naukova Dumka. [in Russian].

Borskiy, M. N. (2017). Vidovaya samostoyatel'nost' i struktura Achillea micranthoides Klok. [Species autonomy and structure of Achillea micranthoides Klokov]. Vestnik Moskovskogo gosudarstvennogo oblastnogo universiteta. Seriya: Estestvennye nauki - Bulletin of the Moscow Region State University. Series: Natural Sciences, (4), 15-20. [in Russian]. https://doi.org/10.18384/2310-7189-2017-4-15-20

Duyn, I. F., Mazulin, O. V., Lukina, & I. A. (2018). Nakopychennia vitaminu K1 u travi perspektyvnykh vydiv rodu Achillea L. [The accumulation of vitamin K1 in herbs of promising species of the Achillea l. Genus]. Molodyi vchenyi – YoungScientist, (1), 45-48. [in Ukrainian].

Duyun, I. F. Mazulin, A. V. Belenichev, I. F., & Abramov, A. V. (2019). Eksperimental'noe izuchenie gepatoprotektornoi i antioksidantnoi aktivnosti ekstrakta travy Achillea micranthoides Klok. Et Krytzka [Experimental study of hepatoprotective and antioxidant activity Achillea micranthoides Klok. et Krytzka grass extract]. Farmakolohiia ta likarska toksykolohiia – Pharmacology and drug toxicology, 13(1), 51-57. [in Russian].

Serbin, A. G. Kartmazova, L. S. Rudenko, V. P., & Gontovaya, T. N. (2006). Atlas po anatomii rastenii [Atlas of Plant Anatomy]. Khar'kov: Kolorit. [in Russian].

Grytsyk, A. R. Neiko, O. V., & Melnyk, M. V. (2016). Morphological-anatomical study of Achillea L. species in western region of Ukraine. The Pharma Innovation Journal, 5(1), 71-73. https://doi.org/10.21448/ijsm.240706

Ishmuratova, M., Suleimen, Y., Zhanzhaxina, A., & Shaidulla, K. (2018). Anatomicheskoe issledovanie Achillea Salicifolia [Anatomic study of Achillea Salicifolia]. Eksperimental'nye i teoreticheskie issledovaniya v sovremennoi nauke (no. 4, pp. 60-65). Novosibirsk: SibAK. [in Russian].

State Enterprise Ukrainian Scientific Pharmacopoeial Center of Medicines Quality. (2015). Derzhavna Farmakopeia Ukrainy [The State Pharmacopoeia of Ukraine]. (2nd ed., Vol. 1). Kharkiv: Naukovo-ekspertnyi farmakopeinyi tsentr. [in Ukrainian].

Barykina, R. P., Veselova, T. D., Devyatov, A. G., Dzhalilova, Kh. Kh., Il'ina, G. M., & Chubatova, N. V. (2004). Spravochnik po botanicheskoi mikrotekhnike. Osnovy i metody [Handbook of Botanical Microtechnology. Basics and Methods]. Moscow: Izdatel'stvo Moskovskogo universiteta. [in Russian].

Hajibagheri, M. A. N. (1999). Electron Microscopy Methods and Protocols. Vol. 117. Totowa, NJ: Human Press Inc.

Downloads

How to Cite

1.
Duyun IF, Mazulin OV, Oproshanska TV. Study of the anatomical structure of the herbal raw materials of Achillea micranthoides Klok. et Krytzka. Current issues in pharmacy and medicine: science and practice [Internet]. 2020Mar.10 [cited 2024Nov.23];13(1). Available from: http://pharmed.zsmu.edu.ua/article/view/198137

Issue

Vol. 13 No. 1 (2020)

Section

Original research

License

Authors who publish with this journal agree to the following terms:

    1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.     Лицензия Creative Commons
    2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
    3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access)