Comparative pharmacognostic and pharmacological study of Salvia verticillata and Salvia officinalis leaves to establish the prospect of creating a new medicines

Authors

  • M. M. Myha National University of Pharmacy, Kharkіv, Ukraine,
  • O. M. Koshovyi National University of Pharmacy, Kharkiv, Ukraine,
  • O. V. Hamulia National University of Pharmacy, Kharkiv, Ukraine,
  • Yu. V. Verkhovodova National University of Pharmacy, Kharkiv, Ukraine,
  • I. V. Kireiev National University of Pharmacy, Kharkiv, Ukraine,
  • A. M. Komisarenko National University of Pharmacy, Kharkiv, Ukraine,

DOI:

https://doi.org/10.14739/2409-2932.2020.1.198136

Keywords:

genus Salvia, non-pharmacopoeial species, leaves, microscopic analysis, chemical composition, anti-inflammatory activity

Abstract

The raw material base of medicinal plants in Ukraine is sufficient only for half of the pharmacopoeial species. Most of them are growing in insufficient quantities and there is a need to import them. In the conditions of import dependence and scarcity of domestic raw materials, the search for new sources of biologically active substances among the representatives of the Ukrainian flora is an urgent aim of modern pharmaceutical science.

The purpose of the research is to conduct comparative pharmacognostic and pharmacological studies of S. verticillata and S. officinalis leaves to determine the possibility of using non-pharmacopoeial species in pharmaceutical and medical practice.

Materials and methods. The objects of the study are S. verticillata and S. officinalis leaves, which was harvested in the botanical garden of the I. Franko Lviv National University. The morphological and anatomical structure of the species leaves was studied by light microscopy. The study of macro- and microelement composition in the S. officinalis and S. verticillata leaves was carried out by atomic emission spectrographic method. Determination of the qualitative composition and quantitative content of the main groups of biologically active substances was carried out by HPLC. The quantitative determination of phenolic compounds was also carried out by spectrophotometric method. The anti-inflammatory activity of galenic extracts from the leaves of the studied species was studied using a model of acute inflammatory edema caused by subplant implantation of a carrageenin solution.

Results. Using light microscopy, we studied the key microscopic and diagnostic signs of S. verticillata leaves. The content of 15 micro and macroelements was found in both studied species. 15 and 14 amino acids, 8 and 6 saponins, respectively, were identified in the S. officinalis and S. verticillata leaves. By HPLC, the qualitative composition and quantitative content of phenolic substances in the S. officinalis and S. verticillata leaves (13 and 12 compounds, respectively) was established. The maximum antiexudative effect of the dry extracts from the S  officinalis and S. verticillata leaves was observed at a dose of 50 mg/kg.

The dominant of elements are silicon, phosphorus, magnesium, calcium, sodium and potassium. The total content of trace elements in the leaves of S. verticillata is 1.78 times higher than in the pharmacopeia S. officinalis. The total content of saponins predominates in the leaves of S. verticillata and is 15.09 mg/g, which is 12.1 % more than in the pharmacopeia specie. The total flavonoid content in the leaves of S. verticillata is 1.57 times higher than in the pharmacopeia one S. officinalis, the content of hydroxycinnamic acids is highest in the S. officinalis leaves. The highest total content of all phenolic compounds is characteristic of S. verticillata leaves, which is 37.18 % (1.37 times) more than the pharmacopeia S. officinalis. At a dose of 50 mg/kg the anti-inflammatory effect of the dry extract from the S. verticillata leaves was comparable to the activity of the substance from the S. officinalis leaves.

Conclusions. As a result of comparative pharmacognostic and pharmacological study of S. verticillata and S. officinalis leaves, it was found that S. verticillata is a promising species for introduction into medical and pharmaceutical practice, precisely as a source of phenolic compounds. The anti-inflammatory effect of the dry extract of S. verticillata leaves was compared with that of the substance of S. officinalis leaves, which indicates the possibility of creating a new anti-inflammatory medicine based on it.

References

Borodina, N. V., Kovalov, V. M., Koshovyi, O. M., & Hamulia, O. V. (2019). Mikroskopichni doslidzhennia pahoniv Salix cinerea L. flory Ukrainy [Microscopic research of shoots of the Salix cinerea L. of Ukrainian flora]. Current issues in pharmacy and medicine: science and practice, 12(3), 276-285. [in Ukrainian]. https://doi.org/10.14739/2409-2932.2019.3.184189

Verkhovodova, Y. V., Kireyev, I. V., Koshovyi, O. M., & Myha, M. M. (2019). Doslidzhennia antyeksudatyvnoi aktyvnosti ekstraktiv z lystia Salvia officinalis [Investigation of anti-exudative activity of Salvia officinalis leaves extracts]. Medychna ta klinichna khimiia, 21(4), 54-60. [in Ukrainian]. https://doi.org/10.11603/mcch.2410-681X.2019.v.i4.10839

State Enterprise Ukrainian Scientific Pharmacopoeial Center of Medicines Quality. (2015). Derzhavna Farmakopeia Ukrainy [The State Pharmacopoeia of Ukraine]. (2nd ed., Vol. 1). Kharkiv: Naukovo-ekspertnyi farmakopeinyi tsentr. [in Ukrainian].

Stefanov, О. V. (2001). Doklinichni doslidzhennia likarskyh zasobiv [Preclinical studies of medicines]. Kyiv : Avitzena. [in Ukrainian].

Kovalenko, V. N. (Ed.). (2014). Kompendium 2014 – lekarstvennyye preparaty [Compendium 2014 – medicines]. Kyiv: MORION. [in Ukrainian].

Koshovyi, O. M., Vovk, G. V., Akhmedov, E. Yu., & Komissarenko, A. N. (2015). Issledovanie khimicheskogo sostava i farmakologicheskoi aktivnosti ekstraktov, poluchennykh pri kompleksnoi pererabotke listev shalfeya lekarstvennogo [The study of the chemical composition and pharmacological activity of salvia officinalis leaves extracts getting by complex processing]. Azerbaijan Pharmaceutical and Pharmacotherapy Journal, (1), 30-34. [in Russian].

Koshovyi, O. M., Perederiy, E. A., Kovaleva, A. M., & Komisarenko, A. M. (2010). Doslidzhennia fenolnykh spoluk lystia shavlii likarskoi [Investigation of phenolic compounds of sage leaf medicinal]. Farmatsevtychnyi chasopys, (1), 17-19. [in Ukrainian]. https://doi.org/10.11603/2312-0967.2010.1.2769

Koshovyi, O. M. (2013). Suchasni pidkhody do stvorennia likarskykh zasobiv na osnovi roslyn rodiv Evkalipt ta Shavliia (Dis. dokt. farm. nauk) [Contemporary approaches to the new remedies on the basis of the plants from Eucalyptus and Salvia genera creation (Doctoral dissertation)]. National Pharmaceutical University, Kharkiv. [in Ukrainian].

Koshovyi, O. M., Komisarenko, A. M., Kovaleva, A. M., & Mudrik, I. M. (2005). Mikroelementnyi, aminokyslotnyi ta polisakharydnyi sklad lystia evkalipta [The microelement, amino acid and polysaccharide composition of eucalyptus leaves]. Fitoterapiya. Chasopys, (3), 59-62. [in Ukrainian].

Koshovyi, O. M. (2012). Fenolnyi sklad deiakykh predstavnykiv pidrodu Sclarea rodu Salvia [Phenolic composition of some representatives of the subspecies Sclarea of the genus Salvia]. Current issues in pharmacy and medicine: science and practice, (3), 11-14. [in Ukrainian].

Myha, M. M., Koshovyi, O. M., Ilina, T. V., Borodina, N. V., & Skybitska, M. I. (2019). Doslidzhennia fenolnykh spoluk lystia nefarmakopeinykh vydiv rodu Salvia flory Ukrainy [Research in phenolic compounds in leaves of non-pharmacopoeial species of the genus Salvia from Ukrainian flora]. Current issues in pharmacy and medicine: science and practice, 12(3), 291-297. [in Ukrainian]. https://doi.org/10.14739/2409-2932.2019.3.184191

Minarchenko, V. M., & Butko, A. Yu. (2017). Doslidzhennia vitchyznianoho rynku likarskykh zasobiv roslynnoho pokhodzhennia [Study on domestic market of medicinal products of plant origin]. Farmatsevtychnyi zhurnal, (1), 30-36. [in Ukrainian].

Baranauskiene, R. Dambrauskiene, E., Venskutonis, P. R., Viskelis, P. (2011). Influence of harvesting time on the yield and chemical composition of sage (Salvia officinalis L.). Foodbalt, 104-109.

Firuzi, O., Miri, R., Asadollahi, M., Eslami, S., & Jassbi, A. R. (2013). Cytotoxic, Antioxidant and Antimicrobial Activities and Phenolic Contents of Eleven Salvia Species from Iran. Iranian Journal of Pharmaceutical Research, 12(4), 801-810. https://doi.org/10.22037/IJPR.2013.1364

Janicsak, G., Zupko, I., Mathe, I., & Hohmann, J. (2010). Comparative Study of the Antioxidant Activities of Eleven Salvia Species. Natural Product Communications, 5(2), 227-230. https://doi.org/10.1177/1934578X1000500211

Jash, S. K., Gorai, D., & Roy, R. (2016). Salvia genus and triterpenoids. International Journal of Pharmaceutical Sciences and Research, 7(12), 4710-4732. https://doi.org/10.13040/ijpsr.0975-8232.7(12).4710-32

Jassbi, A. R., Zare, S., Firuzi, O., & Xiao, J. B. (2016). Bioactive phytochemicals from shoots and roots of Salvia species. Phytochemistry Reviews, 15(5), 829-867. https://doi.org/10.1007/s11101-015-9427-z

Ilina, T., Kashpur, N., Granica, S., Bazylko, A., Shinkovenko, I., Kovalyova, A., Goryacha, O., & Koshovyi, O. (2019). Phytochemical Profiles and In Vitro Immunomodulatory Activity of Ethanolic Extracts from Galium aparine L. Plants-Basel, 8(12), Article 541. https://doi.org/10.3390/plants8120541

Kamatou, G., Viljoen, A., & Steenkamp, P. (2010). Antioxidant, anti-inflammatory activities and HPLC analysis of South African Salvia species. Planta Medica, 76(12), 1233-1233.

Kohevoi O. N. (2011). Amino-acid and monosaccharide compositions of Salvia officinalis leaves. Chemistry of Natural Compounds, 47, 492-493. https://doi.org/10.1007/s10600-011-9976-3

Koshovyi, O. M., Zagayko, A. L., Kolychev, I. O., Akhmedov, E. Yu., & Komissarenko, A. N. (2016). Phytochemical study of the dry extract from bilberry leaves. Azerbaijan Pharmaceutical and Pharmacotherapy Journal, 1, 18-23.

Osmachko, A. P., Kovaleva, A. M., Ili'ina, T. V., Koshovyi, O. N., Komisarcnko, A. M., & Akhmedov, E. Yu. (2017). Study of macro- and microelements composition of Veronica longifolia L. herb and Veronica teucrium L. herb and rhizomes, and extracts obtained from these species. Azerbaijan Pharmaceutical and Pharmacotherapy Journal, 1, 24-28.

Raal, A., Orav, A., & Arak, E. (2007). Composition of the essential oil of Salvia officinalis L. from various European countries. Natural Product Research, 21(5), 406-411. https://doi.org/10.1080/14786410500528478

Shynkovenko, I. L., Ilyina, Т. V., Kovalyova, А. М., Goryacha, O. V., Golembiovska, O. I., & Koshovyi, О. M. (2018). Saponins of the extracts of Galium aparine and Galium verum. Visnyk farmatsii, (4), 16-23. https://doi.org/10.24959/nphj.18.2225

Xu, J. P., Wei, K. H., Zhang, G. J., Lei, L. J., Yang, D. W., … Li, M. H. (2018). Ethnopharmacology, phytochemistry, and pharmacology of Chinese Salvia species: A review. Journal of Ethnopharmacology, 225, 18-30. https://doi.org/10.1016/j.jep.2018.06.029

How to Cite

1.
Myha MM, Koshovyi OM, Hamulia OV, Verkhovodova YV, Kireiev IV, Komisarenko AM. Comparative pharmacognostic and pharmacological study of Salvia verticillata and Salvia officinalis leaves to establish the prospect of creating a new medicines. Current issues in pharmacy and medicine: science and practice [Internet]. 2020Mar.10 [cited 2024May24];13(1). Available from: http://pharmed.zsmu.edu.ua/article/view/198136

Issue

Section

Original research