Spectrophotometric determination of a substance trifusol in a veterinary suppository

Authors

  • K. H. Klosova Zaporizhzhia State Medical University, Ukraine,
  • K. P. Miedviedieva Zaporizhzhia State Medical University, Ukraine,
  • S. O. Vasiuk Zaporizhzhia State Medical University, Ukraine,
  • I. V. Bushuieva Zaporizhzhia State Medical University, Ukraine,

DOI:

https://doi.org/10.14739/2409-2932.2020.1.198123

Keywords:

spectrophotometry, trifuzol, veterinary suppository, quantitative determination

Abstract

 

The purpose of the work was to develop of a method for the quantitative determination of piperidinium 2-[5-(2-furyl)-4-phenyl-1,2,4-triazol-3-ylthio] acetate (trifuzol) as part of a dosage form – an effervescent intrauterine suppository using spectrophotometry in the ultraviolet region spectrum and its validation according to State Pharmacopoeia of Ukraine.Materials and methods. The study was used a working standard sample of trifuzol, intrauterine effervescent veterinary suppositories, 1.0 g of trifuzol, as a solvent – purified water. Analytical equipment: Specord 200 spectrophotometer, electronic scales ABT-120-5DM, ultrasonic bath ELMASONICE 60 H, class A measuring dishes. Method of spectrophotometric analysis was used.Results. A spectrophotometric method has been developed and validated for the quantitative determination of trifuzol in a dosage form – an intrauterine effervescent veterinary suppository, based on measuring the absorption of an aqueous solution of the drug at 278 nm. The methodology tally the requirements of State Pharmacopoeia of Ukraine for such validation characteristics as specificity, linearity, precision, correctness, and robustness. The analysis of the predicted total uncertainty of the analysis was showed the reproducibility of the method and the possibility of its application in other laboratories.Conclusions. A method for the quantitative determination of trifuzol in the composition of the dosage form, an effervescent intrauterine suppository, according to the requirements of State Pharmacopoeia of Ukraine, was developed and validated. It was proved that according to such validation characteristics as linearity, specificity, precision, correctness, and robustness, the technique is correct. 

References

Parchenko, V. V. Panasenko, O. I., Knysh, Ye. H., Vasiuk, S. O., & Tarkhanova, O. O. (2009). Yakisne ta kilkisne vyznachennia piperydynii 2-[5-(furan-2-il)-4-fenil-1,2,4-triazol-3-iltio]atsetatu v 1% ta 2,5% rozchynakh [Qualitative and quantitative determination of piperidine 2-[5-(furan-2-yl)-4-phenyl-1,2,4-triazol-3-ylthio] acetate in 1% and 2.5% solutions]. Zaporozhye medical journal, 11(3), 111-113.

State Enterprise Ukrainian Scientific Pharmacopoeial Center of Medicines Quality. (2014). Derzhavna Farmakopeia Ukrainy [The State Pharmacopoeia of Ukraine] (2nd ed., Vol. 1). Kharkiv: Naukovo-ekspertnyi farmakopeinyi tsentr. [in Ukrainian].

State Enterprise Ukrainian Scientific Pharmacopoeial Center of Medicines Quality. (2015). Derzhavna Farmakopeya Ukrayiny [The State Pharmacopoeia of Ukraine] (2nd ed., Vol. 3). Kharkiv: State Enterprise Ukrainian Scientific Pharmacopoeial Center of Medicines Quality. [in Ukrainian].

State Enterprise Ukrainian Scientific Pharmacopoeial Center of Medicines Quality. (2001). Derzhavna Farmakopeia Ukrainy [The State Pharmacopoeia of Ukraine] (1nd ed., Add. 1-4.). Kharkiv: State Enterprise Ukrainian Scientific Pharmacopoeial Center of Medicines Quality. [in Ukrainian].

Bushuieva, I. V., & Klosova, K. H. (2019). Vnutrishnomatkovyi shypuchyi veterynarnyi supozytorii. Ukraine Patent UA 136849. Retrieved from https://base.uipv.org/searchINV/search.php?action=viewdetails&IdClaim=261727 [in Ukrainian].

Bushuieva, I. V., Knysh, Ye. H., Panasenko, O. I., & Parchenko, V. V. (2014). Veterynarnyi supozytorii [Veterinary Suppository]. Ukraine Patent UA 92116. Retrieved from https://base.uipv.org/searchINV/search.php?action=viewdetails&IdClaim=203146 [in Ukrainian].

Grisodub, A. I. (2016). Standartizovannyye protsedury validatsii metodik kontrolya kachestva lekarstvennykh sredstv [Standardized procedures for the validation of drug quality control methods]. Kharkiv: State Enterprise Ukrainian Scientific Pharmacopoeial Center of Medicines Quality. [in Russian].

Grisodub, A. I., Zvolinskaya, N. N., Arhipova, N. N., Leont'ev, D. A. Denisenko, N. V. & Dotsenko, T. N. (2004). Vosproizvodimost' farmakopeynykh spektrofotometricheskikh metodov kolichestvennogo opredeleniya lekarstv v raznykh laboratoriyakh [Reproducibility of pharmacopeia spectrophotometric methods of quantitative determination of drugs in different laboratories]. Farmakom, (2), 20-34. [in Russian].

Grisodub, A. I., Leont'ev, D. A., Denisenko N. N., & Podpruzhynnikov, Yu. V. (2004). Standartizirovannyye protsedury dlya proverki metodov kontrolya kachestva lekarstvennykh sredstv s ispol'zovaniyem standartnogo metoda [Standardized procedures for the validation of drug quality control methods using standard method]. Farmakom, (3), 3-14. [in Russian].

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How to Cite

1.
Klosova KH, Miedviedieva KP, Vasiuk SO, Bushuieva IV. Spectrophotometric determination of a substance trifusol in a veterinary suppository. Current issues in pharmacy and medicine: science and practice [Internet]. 2020Mar.10 [cited 2024Dec.3];13(1). Available from: http://pharmed.zsmu.edu.ua/article/view/198123

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Original research