Microscopic research of medicinal plant raw material of Achillea ochroleuca Ehrh.

Authors

  • H. P. Smoilovska Zaporizhzhia State Medical University, Ukraine,
  • O. K. Yerenko Zaporizhzhia State Medical University, Ukraine,
  • T. V. Khortetska Zaporizhzhia State Medical University, Ukraine,
  • I. F. Duiun Zaporizhzhia State Medical University, Ukraine,
  • O. V. Mazulin Zaporizhzhia State Medical University, Ukraine,

DOI:

https://doi.org/10.14739/2409-2932.2019.1.158984

Keywords:

Achillea ochroleuca Ehrh., microscopy, diagnostic features

Abstract

Achillea millefolium L. is an official plant both in Ukraine and in other countries of Europe and Asia. Plants of the genus Achillea L. are diverse in component composition and contain a complex of biologically active compounds: essential oils, polyphenols, vitamins, carotenoids, polysaccharides, trace elements. Medicines, which include various extracts of yarrow, are used as hemostatic, anti-inflammatory, gastroenteric drugs, used to treat the hepatobiliary system, diabetes, diseases of the paradont. Extracts of yarrow reveal regenerative, cytotoxic, anabolic, antioxidant effects. At the present time, the Achillea L. species, which are growing in Ukraine, are still poorly studied. One of the representatives of the genus Achillea L. is an Achillea ochroleuca Ehrh., which has the enough raw material base and a similar composition of biologically active substances comparing to the Achillea millefolium L. Therefore, determination of the diagnostic features of Achillea ochroleuca Ehrh. in a comparison with the official medicinal raw material of Achillea millefolium L. is actual for further introduction to modern medical practice.

The aim of our research was to determine the diagnostic microscopic feature of the medical plant raw material of Achillea ochroleuca Ehrh. for the plant material identification.

Material and methods. Within microscopic study we have used herb of Achillea ochroleuca Ehrh., picked during flowering period. Fresh plant raw material has been fixed in mixture: glycerin, ethyl alcohol 96 %, purified water (1:1:1). The research has been done with using chloral hydrate according to methods recommended by State Pharmacopeia of Ukraine.

Results and discussion. While carrying out microscopic studies of medicinal plant raw material we payed attention to the diagnostic characters of the stem of the plant, to the structure of epidermal cells, availability, number and type of stomata, features for covering and glandular trichomes, leave structure, cells of the epidermis of the wrapper and the petal.

Conclusions. The general anatomical signs of Achillea ochroleuca Ehrh. and Achillea millefolium L. are the parenchymal-prozenhymal epidermis of the stem and the leaf structure of isolateral type. Covering and glandular trichomes which are specific for yarrow are presented on the epidermis of stems, leaves, flowers. Stomata apparatus is of anomocyte type. The distinctions for Achillea ochroleuca Ehrh. are expressed in the thickening of the epidermis cells and the parenchyma of the primary cortex. This species is characterized by a greater number of glandular trichomes with dark colored contents. The microscopic features identified during the study reliably identify plant raw materials of a morphologically close species of the genus Achillea L.

References

Turkmenoglu, F. P., Agar, O. T., Akaydin, G., Hayran, M., & Demirci, B. (2015). Characterization of Volatile Compounds of Eleven Achillea Species from Turkey and Biological Activities of Essential Oil and Methanol Extract of A. hamzaoglui Arabacı & Budak. Molecules, 20(6), 11432–58. doi: 10.3390/molecules200611432.

Szymanski, M., Witkowska-Banaszczak, E., Klak, N., Marciniak, K., Wolowiec, T., & Szymanski, A. (2014). Effects of Trace Elements on Polyphenolic Compounds in Millefolii Herba. Pol. J. Environ. Stud., 23(2), 459–466.

Akcin, T., & Akcin, A. (2014). Achene micromorphology of seven taxa of Achillea L. (Asteraceae) from Turkey. Bangladesh J. Plant Taxon, 21(1), 19–25. doi: https://doi.org/10.3329/bjpt.v21i1.19253

Serdar, G., Sökmen, M., Demir, E., Sökmen, A., & Bektaş, E. (2015). Extraction of antioxidative principles of Achillea biserrata M. Bieb. and chromatographic analyses. International Journal of Secondary Metabolite, 2(2), 3–15. doi: 10.21448/ijsm.240706

Agar, O.T., Dikmen, M., Ozturk, N., Yilmaz, M. A., Temel, H., & Turkmenoglu, F. P. (2015). Comparative Studies on Phenolic Composition, Antioxidant, Wound Healing and Cytotoxic Activities of Selected Achillea L. Species Growing in Turkey. Molecules, 20(10), 17976–8000. doi: 10.3390/molecules201017976

Derzhavne pidpryvo «Ukrainskyi naukovyi farmakopeinyi tsentr yakosti likarskykh zasobiv» (2014). Derzhavna farmakopeia Ukrainy [State Pharmacopoeia of Ukraine], Vol. 3. Kharkiv. [in Ukrainian].

Kovalov, V. M., Marchyshyn, S. M., Khvorost, O. P., et al. (2014). Praktykum z identyfikatsii likarskoi roslynnoi syrovyny [Workshop on identify catoin of medical plants]. Ternopil : TDMU. [in Ukrainian].

Grytsyk, A. R., Neiko, O. V., & Melnyk, M. V. (2016). Morphological-anatomical study of Achillea L. species in western region of Ukraine. The Pharma Innovation Journal, 5(1), 71–73.

Albayrak, S. (2013). The Volatile Compounds and Bioactivity of Achillea sieheana Stapf. (Asteraceae). Iran J Pharm Res, 12(1), 37–45.

Vardanyan, L. R., Atabekyan, L. V., Hayrapetyan, S. A., & Vardanyan, R. L. (2018). Antioksidantnaya aktivnost' etilacetatnogo e'kstrakta raznykh vidov tysyachelistnika (Achillea L.) [Antioxidant activity of ethyl acetate extract of different yarrow species (Achillea L.)]. Khimiya rastitel'nogo syr'ya, 3, 61–68. doi: 10.14258/jcprm.2018033697.

Ali Reza Ladan Moghadam (2017). New compound from the aerial parts of Achillea millefolium. International Journal of Food Properties, 20(9), 2041–2051. doi: 10.1080/10942912.2016.1230747

Taskin, T., Ozakpinar, O. B., Curbur, B., Uras, F., Gürer, S. Ü., & Bitiş, L. (2016). Identification of phenolic compounds and evaluation of antioxidant, gutantimicrobial and cytotoxic effects of the endemic Achillea multifida. Indian journal of traditional knowledge, 15(4), 594–603.

Fedosov, A. I., Kyslychenko, V. S., & Kyslychenko, O. А. (2014). Kilkisne vyznachennia biolohichno aktyvnykh rechovyn z antymikrobnoiu diieiu u shlunkovykh zborakh [Quantitative determination of biologically active substances with the antimicrobial action in gastric teas]. Klinichna farmatsiіa, 18(464), 63–65. [in Ukrainian].

Pereiraa, J. M., Peixoto, V., Teixeiraa, A., Sousa, D., Barros, L., Ferreira, I. C. F. R., & Vasconcelos, M. H. (2018). Achillea millefolium L. hydroethanolic extract inhibits growth of human tumor cell lines by interfering with cell cycle and inducing apoptosis. Food and Chemical Toxicology, 118, 635–644. doi: 10.1016/j.fct.2018.06.006

Derzhavne pidpryiemstvo «Ukrainskyi naukovyi farmakopeinyi tsentr yakosti likarskykh zasobiv» (2015). Derzhavna farmakopeia Ukrainy [State Pharmacopoeia of Ukraine], Vol. 1. Kharkiv. [in Ukrainian].

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How to Cite

1.
Smoilovska HP, Yerenko OK, Khortetska TV, Duiun IF, Mazulin OV. Microscopic research of medicinal plant raw material of Achillea ochroleuca Ehrh. Current issues in pharmacy and medicine: science and practice [Internet]. 2019Mar.11 [cited 2024Dec.21];(1). Available from: http://pharmed.zsmu.edu.ua/article/view/158984

Issue

No. 1 (2019)

Section

Original research

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