The study of anatomical diagnostic signs of raw material of some Agastache species as quality indicators for standardization
Keywords:Agastache foeniculum, Agastache urticifolia, microscopy, anatomymetric indices, standardization of raw materials
AbstractThe basic stage of medicinal plant material standardization is the determination of authenticity by external and microscopic characteristics. There is a wide range of plants widely used in folk medicine, which have not yet entered the State Pharmacopoeia of Ukraine. Great interest is paid to representatives of the genus Agastache J.Clayton ex Gronov, which are widely used in eastern medicine for inflammatory processes of the gastrointestinal tract and urinary system treatment and also for colds treatment; externally - for dermatitis of fungal origin and seborrhea treatment. These representatives are promising for cultivation in Ukraine.The aim of our research is the microscopic analysis of the vegetative and generative organs structure of the different aged individuals Agastache foeniculum and A. urticifolia, the selection of a complex of anatomical diagnostic features that can be used in identity and quality indices establishment.Materials and methods. Investigation of anatomical indices of leaves, petioles, stems of different age groups of A. foeniculum and A. urticifolia at juvenile and generative stages of ontogenetic development was conducted using a microscope XSP-146TR. The collections fund was used from the Botanical Garden n.а. acad. O.V. Fomin Kyiv National University named after. T. Shevchenko. Mira-preparations were made from the surface of freshly assembled and fixed raw materials. Samples of raw materials were collected on experimental sites of the Botanical Garden. acad. O.V. Fomin Kyiv National University named after. T. Shevchenko in early May, June 2016-2017.Results. A comparative microscopic analysis of the structure of vegetative and generative organs of different species of A. foeniculum and A. urticifolia has been carried out, correlations have been made in the changes of anatomic indicators of different organs according to the stage of ontogenetic development of plants. It is established that these changes are primarily due to the increase of vegetative organs in the size and are manifested in particular in reducing the abundance of their pubescence in the transition of individuals to the generative stage, particularly the number of glandular trichomes is smaller on generative stage for both species. Conclusions. According to the results of the anatomical research, it can be argued that determining the type of pubescence, its localization and abundance can serve as a reliable criterion for the diagnosis of the studied species, as well as the establishment of identity and quality indices of medicinal plant raw materials during standardization.
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