Optimization of L-tryptophan and thiotriazoline compound analysis by high-performance liquid chromatography
DOI:
https://doi.org/10.14739/2409-2932.2018.2.133170Keywords:
L-tryptophan, thiotriazoline, combination drugs, model mix, high-performance liquid chromatographyAbstract
Introduction. An actual task of modern medicine is the development of treatment methods of the central nervous system diseases. The solution of this problem was the creation of new, more effective neuropsychotropic drug that shows pronounced antidepressant, nootropic, neuroprotective and antioxidant effects based on the fixed combination of L-tryptophan with thiotriazoline. One of the steps in creating a new drug is the standardization of active ingredients.
Today, much attention is being paid to new physical-chemical methods of drugs study, including high-performance liquid chromatography (HPLC). Increasingly, the HPLC method is used for the determination of active ingredients of organic nature, both in mono-preparations and in combined dosage forms.
Therefore, we have proposed the HPLC method for new combined L-tryptophan and thiotriazoline drug in order to determine active substances based on their effects.
The purpose of our study is to develop a method for the simultaneous standardization of L-tryptophan and thiotriazoline in an artificial compound by high-performance liquid chromatography, in particular the selection of the mobile and stationary phase.
Materials and methods. In the first stage of our work, a selection of phase and eluents was carried out for L-tryptophan and thiotriazoline model compound in a ratio of 1:1. The analysis method of thiotriazoline in the reverse phase (C 18) using the eluents, which are water-methanol compounds, was taken as a basis.
Results. In the course of study, it was found that the retention volume of both tryptophan and thiotriazoline decreased, and the peak distribution coefficient increased with the number of theoretical plates with increasing content of methanol in phosphate buffer compound. Therefore, as an eluent, attention was drawn to a solution of 20% methanol with 80 % phosphate buffer. Under these conditions, the tryptophan retention volume was 15.11 ml, thiotriazoline – about 6.05 ml, the efficacy of the chromatographic column, calculated on the tryptophan peak of about 3300 theoretical plates.
Findings: A highly sensitive technic was developed as part of the study for the simultaneous determination of L-tryptophan and thiotriazoline in a model mixture by HPLC (high performance liquid chromatography method, that is planned to be used in further study, was developed as a part of the study. The method is developed not only for L-tryptophan and thiotriazoline standardization in the combined drug, but also for their determination in biological fluids.
References
Fesenko, Yu. A., Churilov, L. P., Khudik, V. A., Stroev, Yu. I., Danilenko, O. V., & Sobolevskaya, P. A. (2018). Nevrozy i stress [Neurosis and stress]. Saint Petersburg: Foliant. [in Russian].
Kovalenko, V. Kh. (ed.) (2013) Kompendium 2013 – lekarstennye preparaty [Kompendium 2013 – medications]. Kyiv: Morion. [in Russian].
Kucherenko, L. І., Borsuk, S. O., & Belenіchev, І. F. (2016) Patent 112513 / № a201604961 Ukraina, МПК (2016), А61К 31/405 (2006.01) А61К 31/41 (2006.01) А61 Р 25/00 А61Р 25/28 (2006.01). Kombіnovanyi lіkarskyi zasіb anksіolіtychnoi, stres-protektyvnoi, nootropnoi antyoksydantnoi dіi [Anxiolytic, stress-protective, nootropic, and anti-oxidant combination drug]. Biuleten, 12. [in Ukrainian].
Mazur, I. A., Voloshin, N. A., & Chekman, I. S. (2005) Tiotriazolin [Thiotriazoline]. Zaporozh'e, Lvov: Nautilus [in Russian].
Georgievskij, G. V. (2011) Razrabotka kompleksa fiziko-khimicheskikh metodik, obespechivayuschikh sozdanie i kontrol' kachestva original'nykh otechestvennykh preparatov, proizvodnykh 1,2,4-triazola [Development of a complex of physico-chemical methods that provide creation and control of the quality of original domestic preparations, derivatives of 1,2,4-triazole]. Zaporozhye medical journal, 1, 58–69. [in Russian].
Pakhomov, V. P. (2003) Khromatohrafiia u khimiko-farmatsevtychnykh doslidzhenniakh [Chromatography in chemical and pharmaceutical research]. Khimiko-farmactvticheskij zhurnal, 37(8), 55–56. [in Ukrainian].
Oiestad, E. L., Johansen, U., & Christophersen, A. S. (2007) Drug screening of preserved oral fluid by liquid chromatography-tandem mass spectrometry. Clin Chem., 53, 300–309. doi: 10.1373/clinchem.2006.074237.
Kucherenko, L. I., Khromylyova, O. V., Moryak, Z. B., & Tkachenko, H. I. (2014) Pidbir optymalnykh umov analizu shtuchnoi sumishi izoniazydu ta tiotryazolinu metodom vysokoefektyvnoi ridynnoi khromatohrafii [Choice of optimal conditions of Isoniazid and Thiotriazolin artifi cial mixture analysis by high-performance liquid chromatography method]. Zaporozhye medical journal, 2, 118–120. [in Ukrainian]. doi: https://doi.org/10.14739/2310-1210.2014.2.25524.
Kucherenko, L. I., Nimenko, Н. R., Vashchenko, O. V., & Vashchenko, V. V. (2016). Shchodo sumisnoho vyznachennia karbamazepinu ta tiotryazolinu v modelnii sumishi metodom VERKh. Povidomlennia 1: pidbir fazy dlia sumisnoho vyznachennia karbamazepinu ta tiotryazolinu u modelnoi sumishi metodom VERKh [Carbamazepine and thiotriazoline simultaneous definition in model mixture by HPLC. Message 1: phase selection for the simultaneous determination of carbamazepine and thiotriazolin in model mixture by high performance liquid chromatography]. Farmatsevtychnyi chasopys, 1, 54–58. [in Ukrainian].
Derzhavne pidpryiemstvo«Naukovo Ekspertnyi farmakopeinyi tsentr» (2015) Derzhavna Farmakopeia Ukrainy [State Pharmacopoeia of Ukraine]. Kharkiv. [in Ukrainian].
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